發(fā)布時(shí)間：2018-07-03 07:55

  本文選題：視網(wǎng)膜新生血管 + 奧曲肽��； 參考：《山西醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的：在高氧誘導(dǎo)的視網(wǎng)膜新生血管模型的小鼠玻璃體腔注射奧曲肽(OA),觀察奧曲肽對(duì)視網(wǎng)膜新生血管形成的抑制作用,及其抑制效果是否具有劑量依賴性。 方法：將鼠齡7 d的小鼠40只隨機(jī)分為正常組(N組)和高氧組(O組),高氧組分為對(duì)照組(O0組)和實(shí)驗(yàn)組(O1、02、03組)。正常組置于空氣中,其他4組置于密閉氧箱中飼養(yǎng)5天后,返回正常環(huán)境飼養(yǎng),正常組和高氧對(duì)照組玻璃體腔注射生理鹽水3μl,實(shí)驗(yàn)組玻璃體腔注射不同劑量奧曲肽,分別為0.02μg、0.1μg、0.5μg各3μl。7天后處死小鼠,行視網(wǎng)膜鋪片觀察視網(wǎng)膜血管形態(tài),組織切片觀察并計(jì)數(shù)突破視網(wǎng)膜內(nèi)界膜的血管內(nèi)皮細(xì)胞核數(shù)目。 結(jié)果： 1.視網(wǎng)膜血管形態(tài)觀察：正常組(N組)視網(wǎng)膜血管均勻分布,管徑較粗,分支良好,周邊視網(wǎng)膜血管網(wǎng)結(jié)構(gòu)清晰。高氧對(duì)照組(O0)可見(jiàn)視網(wǎng)膜血管分布不均勻,走行僵直,管徑變細(xì),視盤周圍毛細(xì)血管閉塞,可見(jiàn)大片無(wú)灌注區(qū),周邊視網(wǎng)膜分支小血管狹窄,甚至閉塞,可見(jiàn)無(wú)灌注區(qū)。實(shí)驗(yàn)組(O1、O2、O3)隨著奧曲肽劑量增加視網(wǎng)膜血管分布和形態(tài)逐漸改善,03組視網(wǎng)膜血管分布均勻,分支大致走行正常,周邊視網(wǎng)膜血管網(wǎng)結(jié)構(gòu)基本清晰。 2.視網(wǎng)膜新生血管計(jì)數(shù)：高倍鏡(40×10)下可見(jiàn)每組平均每張切片突破視網(wǎng)膜內(nèi)界膜的血管內(nèi)皮細(xì)胞核數(shù)依次為(0.75±0.03)個(gè)/HP、(26.40±1.90)個(gè)/HP、(19.37±1.24)個(gè)/HP、(7.12±0.63)個(gè)/HP和(2.38±0.16)個(gè)/HP,N組和O0組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),O0、O1、02和03組間比較差異均具有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：奧曲肽對(duì)高氧誘導(dǎo)的小鼠視網(wǎng)膜新生血管有抑制作用,且具有劑量依賴性。
[Abstract]:Aim: to observe the inhibitory effect of octreotide (OA) on retinal neovascularization in hyperoxia-induced retinal neovascularization model mice and whether the inhibitory effect is dose-dependent. Methods: forty mice aged 7 days were randomly divided into normal group (group N) and hyperoxia group (group O). Hyperoxia group was divided into control group (group O _ 0) and experimental group (group O _ 1o _ 2). Normal group was placed in air, the other four groups were fed in closed oxygen box for 5 days, then returned to normal environment to feed. Normal group and hyperoxia control group were injected with normal saline 3 渭 l into the vitreous cavity, and the experimental group were injected with different doses of octreotide in vitreous cavity. The mice were killed after 0. 02 渭 g / 0. 1 渭 g / g 0. 5 渭 g / g each 3 渭 l. 7 days later, the retinal vascular morphology was observed by retina preparation, and the number of vascular endothelial nuclei which broke through the inner retinal membrane was observed and counted by tissue section. Results: 1. Observation of retinal vascular morphology: normal group (group N) showed uniform distribution of retinal vessels, coarse diameter, good branches and clear structure of peripheral retinal vascular network. In the hyperoxia control group (O _ 0), the retinal vessels were distributed inhomogeneously, the retinal vessels were stiffened, the diameter became thinner, the capillaries around the optic disc were occluded, a large area without perfusion was seen, and the peripheral retinal branches were narrow, even occluded, and no perfusion area could be seen. With the increase of octreotide dose, the distribution and morphology of retinal vessels in the experimental group were gradually improved, the distribution of retinal vessels was uniform, the branches were normal, and the structure of peripheral retinal vascular network was clear. 2. Retinal neovascularization count: under high power microscope (40 脳 10), the average number of endothelial nuclei per section in each group was (0.75 鹵0.03) HPs, (26.40 鹵1.90) rHPs, (19.37 鹵1.24) rHPs, (7.12 鹵0.63) rHP and (2.38 鹵0.16) rHPNs, respectively. Significant (P0.05) the differences between the two groups were statistically significant (P0.05). Conclusion: octreotide can inhibit retinal neovascularization induced by hyperoxia in a dose-dependent manner.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R774.1

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