發(fā)布時間：2018-07-03 01:55

  本文選題：喉乳頭狀瘤 + 免疫組織化學　； 參考：《福建醫(yī)科大學》2010年碩士論文

【摘要】： 目的通過檢測Cox2、Cx26及Stathmin蛋白在喉乳頭狀瘤及聲帶息肉組織中的表達情況,探討它們在喉乳頭狀瘤發(fā)生、發(fā)展過程中的作用及相互關系。 方法采用免疫組織化學技術(PV-9000二步法)檢測Cox2、Cx26及Stathmin蛋白在60例喉乳頭狀瘤和15例聲帶息肉組織中的表達情況,應用Image-ProPlus圖像分析軟件測定免疫組化圖像的平均光密度(Mean Optical Density, MOD),運用SPSS 13.0軟件進行統(tǒng)計學分析,觀察各指標與喉乳頭狀瘤臨床特征之間的關系及其在喉乳頭狀瘤組織中表達的相關性。 結果(1)Cox2及Stathmin蛋白在喉乳頭狀瘤組織中的陽性表達率分別為73.33%、88.33%,均明顯高于它們在聲帶息肉組織中的表達率(33.33%、53.33%),兩者差異有統(tǒng)計學意義(P0.05)。(2)Cx26蛋白在喉乳頭狀瘤組織中的陽性表達率為33.33 %,明顯低于其在聲帶息肉組織中的表達率(73.33 %),兩者差異有統(tǒng)計學意義(P0.05)。喉乳頭狀瘤中Cx26蛋白的表達伴有細胞內定位的改變,Cx26蛋白在聲帶息肉組織中主要表達在細胞膜,而在喉乳頭狀瘤組織中的細胞膜、細胞漿和小部分細胞核中均有陽性表達。(3)Cox2、Cx26及Stathmin蛋白的表達水平與臨床分型、是否復發(fā)、患者性別及年齡均無相關性(P0.05)。(4)喉乳頭狀瘤中,Cox2蛋白與Stathmin蛋白表達呈正相關(r=0.287,P0.05),而Cx26蛋白表達與Cox2及Stathmin蛋白表達呈負相關(r=-0.213、r=-0.184,P0.05)。 結論 1. Cox2蛋白在喉乳頭狀瘤組織中高表達,提示其與喉乳頭狀瘤的發(fā)生可能有密切關系,有望為選擇性Cox2抑制劑預防和治療喉乳頭狀瘤提供依據(jù)。 2.喉乳頭狀瘤組織中Cx26蛋白的表達明顯低于聲帶息肉,且伴有細胞內定位的改變,提示其在喉乳頭狀瘤的發(fā)生、發(fā)展過程可能起重要的作用。 3. Stathmin蛋白在喉乳頭狀瘤組織中高表達,提示其與喉乳頭狀瘤的多發(fā)性、浸潤性可能有密切聯(lián)系,可能成為研究生物治療的方向之一。 4. Cox2蛋白與Stathmin蛋白在喉乳頭狀瘤組織中的表達呈正相關,提示它們可能在喉乳頭狀瘤的發(fā)生、發(fā)展過程中起協(xié)同作用。Cx26蛋白與Cox2蛋白及Stathmin蛋白在喉乳頭狀瘤組織中的表達均呈負相關,可能與它們之間的蛋白質相互作用有關,并可能與喉乳頭狀瘤的發(fā)生、發(fā)展有關。
[Abstract]:Objective to investigate the expression of Cox2Cx26 and Stathmin protein in laryngeal papilloma and vocal cord polyp, and to explore their roles in the development and development of laryngeal papilloma. Methods the expression of Cox2Cx26 and Stathmin protein in 60 cases of laryngeal papilloma and 15 cases of vocal cord polyps were detected by immunohistochemical technique (PV-9000 two-step method). The mean optical density (MOD) of immunohistochemical images was measured by Image-ProPlus image analysis software, and the statistical analysis was carried out by SPSS 13.0 software. To observe the relationship between the indexes and the clinical features of laryngeal papilloma and its expression in laryngeal papilloma. Results (1) the positive expression rates of Cox2 and stathmins in laryngeal papilloma were 73.33 and 88.33, respectively, which were significantly higher than those in vocal cord polyps (33.3353.33%). There was a significant difference between them (P0.05). (2) and Cx26 protein in laryngeal papilloma. The sexual expression rate was 33.33, which was significantly lower than that in vocal cord polyps (73.33%), the difference was statistically significant (P0.05). The expression of Cx26 protein in laryngeal papilloma is associated with the change of intracellular localization. Cx26 protein is mainly expressed in the cell membrane of vocal cord polyp, but in the cell membrane of laryngeal papilloma. (3) the expression level of Cox2Cx26 and Stathmin protein and clinical type, recurrence or not. There was no correlation between sex and age (P0.05). (4). There was a positive correlation between the expression of Cox2 protein and stathmin protein in laryngeal papilloma (P 0.05), but a negative correlation between the expression of Cx26 protein and the expression of Cox2 and stathmin protein (r-0.213r-0.184P0.05). Conclusion 1. The overexpression of Cox2 protein in laryngeal papilloma suggests that Cox2 protein may be closely related to the occurrence of laryngeal papilloma and may provide evidence for the prevention and treatment of laryngeal papilloma with selective Cox2 inhibitor. The expression of Cx26 protein in laryngeal papilloma was significantly lower than that in vocal cord polyp and accompanied by changes in intracellular localization, suggesting that Cx26 protein might play an important role in the occurrence and development of laryngeal papilloma. 3. The high expression of Stathmin protein in laryngeal papilloma suggests that Stathmin protein may be closely related to the multiple and invasive laryngeal papilloma and may be one of the research directions of biotherapy. Cox2 protein was positively correlated with the expression of stathmin protein in laryngeal papilloma, suggesting that Cox2 protein might occur in laryngeal papilloma. The expression of Cox2 protein and Stathmin protein in laryngeal papilloma was negatively correlated with the synergistic effect of Cx26 protein during the development of laryngeal papilloma, which may be related to the protein interaction between them, and to the occurrence and development of laryngeal papilloma.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R739.65

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