本文選題：后囊膜混濁 + 糖尿病�。� 參考：《眼科新進(jìn)展》2014年07期

【摘要】：目的觀察糖尿病兔后囊膜混濁(posterior capsule opacification,PCO)發(fā)生過程中血清及房水中晚期糖基化終末產(chǎn)物(advanced glycation end products,AGE)和胰島素樣生長因子-1(insulin-like growth factor 1,IGF-1)的含量變化,同時觀察去整合素echistatin(Ecs)對二者的干預(yù)作用。方法將24只兔隨機(jī)分為對照組、糖尿病組及Ecs組,后2組應(yīng)用四氧嘧啶建立糖尿病模型,所有兔行晶狀體摘出術(shù),Ecs組術(shù)中晶狀體囊袋內(nèi)注入10 mg·L-1Ecs,其余2組注入等量高壓滅菌蒸餾水。分別于術(shù)后10 d、6周提取3組血清及房水,應(yīng)用ELISA法檢測AGE和IGF-1含量。結(jié)果術(shù)后10 d房水中AGE濃度在三組中分別為(0.189±0.200)μg·L-1、(0.467±0.190)μg·L-1、(0.527±0.130)μg·L-1;6周為(0.255±0.640)μg·L-1、(0.716±0.340)μg·L-1、(0.830±0.330)μg·L-1。術(shù)后10 d血清中AGE濃度在對照組、糖尿病組和Ecs組中分別為(0.074±0.013)μg·L-1、(0.270±0.120)μg·L-1、(0.213±0.690)μg·L-1;6周為(0.054±0.010)μg·L-1、(0.166±0.460)μg·L-1、(0.149±0.200)μg·L-1。術(shù)后10 d房水中IGF-1濃度在3組中分別為(0.279±0.080)μg·L-1、(0.914±0.130)μg·L-1、(0.556±0.290)μg·L-1;6周為(0.176±0.030)μg·L-1、(0.508±0.040)μg·L-1、(0.367±0.090)μg·L-1。術(shù)后10 d血清中IGF-1濃度在對照組、糖尿病組和Ecs組中分別為(0.075±0.030)μg·L-1、(0.293±0.160)μg·L-1、(0.305±0.160)μg·L-1;6周為(0.032±0.020)μg·L-1、(0.833±0.570)μg·L-1、(0.788±0.530)μg·L-1。術(shù)后10 d及6周對照組血清及房水中AGE和IGF-1含量均低于糖尿病組及Ecs組(均為P0.05)。糖尿病組及Ecs組中AGE含量比較,差異均無統(tǒng)計學(xué)意義(均為P0.05),糖尿病組及Ecs組中IGF-1含量在血清中差異均無統(tǒng)計學(xué)意義(均為P0.05),但在房水中Ecs組IGF-1含量明顯低于糖尿病組(P=0.003、0.031)。結(jié)論 AGE和IGF-1可能促進(jìn)了糖尿病兔PCO的發(fā)生和發(fā)展,降低房水中IGF-1的含量可能是去整合素Ecs抑制PCO發(fā)展的機(jī)制之一。
[Abstract]:Objective to observe the changes of serum and aqueous humor advanced glycosylation end products (advanced glycation end products age) and insulin-like growth factor 1 (insulin-like growth factor 1 IGF-1) during the development of diabetic rabbit posterior capsular opacification (posterior capsule), and to observe the intervention effect of echistatin (Ecs) on them. Methods Twenty-four rabbits were randomly divided into control group, diabetic group and Ecs group. The latter two groups were treated with alloxan to establish diabetic model. In all rabbits, 10 mg L -1 Ecs was injected into the capsular bag of Ecs group, and the other two groups were injected with distilled water of the same volume of high pressure sterilization. Serum and aqueous humor were extracted 10 days and 6 weeks after operation, and the levels of age and IGF-1 were detected by Elisa. Results the concentration of age in aqueous humor was (0.189 鹵0.200) 渭 g / L, (0.467 鹵0.190) 渭 g / L, (0.527 鹵0.130) 渭 g / L ~ (-1), (0.255 鹵0.640) 渭 g / L ~ (-1), (0.716 鹵0.340) 渭 g / L ~ (-1), (0.830 鹵0.330) 渭 g / L ~ (-1), respectively. The serum age levels were (0.074 鹵0.013) 渭 g / L, (0.270 鹵0.120) 渭 g / L, (0.213 鹵0.690) 渭 g / L ~ (-1), (0.166 鹵0.460) 渭 g / L ~ (-1), (0.149 鹵0.200) 渭 g / L ~ (-1) in the control group, diabetic group and Ecs group 10 days after operation, and (0.054 鹵0.010) 渭 g / L ~ (-1), (0.166 鹵0.460) 渭 g / L ~ (-1), (0.149 鹵0.200) 渭 g / L respectively. The concentrations of IGF-1 in aqueous humor were (0.279 鹵0.080) 渭 g L -1, (0.914 鹵0.130) 渭 g L -1, (0.556 鹵0.290) 渭 g L -1, (0.176 鹵0.030) 渭 g L -1, (0.508 鹵0.040) 渭 g L -1, (0.367 鹵0.090) 渭 g L -1, respectively. The levels of IGF-1 in serum were (0.075 鹵0.030) 渭 g L ~ (-1), (0.293 鹵0.160) 渭 g L ~ (-1), (0.305 鹵0.160) 渭 g L ~ (-1), (0.833 鹵0.570) 渭 g L ~ (-1) and (0.788 鹵0.530) 渭 g L ~ (-1) in control group, diabetic group and Ecs group 10 days after operation, and were (0.032 鹵0.020) 渭 g / L ~ (-1), (0.833 鹵0.570) 渭 g L ~ (-1) and (0.788 鹵0.530) 渭 g / L ~ (-1) respectively. The levels of age and IGF-1 in serum and aqueous humor in the control group were lower than those in the diabetic group and Ecs group 10 days and 6 weeks after operation (P0.05). There was no significant difference in age content between diabetic group and ECs group (P0.05). There was no significant difference in IGF-1 content in serum between diabetic group and ECs group (P0.05), but the level of IGF-1 in aqueous humor of ECs group was significantly lower than that in diabetic group (P0. 0030. 031). Conclusion age and IGF-1 may promote the occurrence and development of PCO in diabetic rabbits, and decreasing the content of IGF-1 in aqueous humor may be one of the mechanisms of deintegrin Ecs inhibiting the development of PCO.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院眼科;
【基金】：國家自然科學(xué)基金項目(編號:81160120)~~
【分類號】：R587.2;R776.1

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