本文選題：FoxM1 + 喉癌��； 參考：《重慶醫(yī)科大學》2011年碩士論文

【摘要】：目的: 1、研究FoxM1在喉癌中的表達情況及臨床意義,為喉癌的發(fā)生發(fā)展、預后評估及尋求新的治療靶點提供實驗依據(jù)。 2、研究姜黃素對喉癌細胞株Hep-2增殖、侵襲、轉(zhuǎn)移能力的影響,為喉癌治療提供新的策略。 3、研究FoxM1在姜黃素作用喉癌細胞株Hep-2前后的表達變化,探討FoxM1在其中的作用。 方法: 1、運用免疫組織化學方法檢測89例喉癌及89例癌旁組織、20例喉乳頭狀瘤組織中FoxM1蛋白的表達情況,分析它們在不同病變組織中的表達情況及與喉癌臨床病理參數(shù)的關(guān)系。 2、運用四甲基偶氮唑藍(MTT)法、侵襲實驗以及遷移實驗檢測經(jīng)姜黃素作用后喉癌細胞Hep-2體外增殖、侵襲和轉(zhuǎn)移能力的變化。 3、運用反轉(zhuǎn)錄-聚合酶鏈反應(yīng)(RT-PCR)檢測姜黃素作用前后喉癌細胞中FoxM1的表達水平。 結(jié)果: 1、FoxM1蛋白表達的陽性率在喉癌、喉乳頭狀瘤及癌旁組織中逐漸降低,其差異有統(tǒng)計學意義(P0.05);FoxM1蛋白的表達隨組織分化程度增高而表達降低(P0.05);隨T分期增高而表達增加(P0.05);有淋巴結(jié)轉(zhuǎn)移組高于無淋巴結(jié)轉(zhuǎn)移組(P0.05),隨臨床分期的增加而表達增加(P0.05)。 2、姜黃素能抑制喉癌細胞Hep-2體外增殖能力,并隨著姜黃素濃度的增加和作用時間的延長,其對Hep-2細胞增殖抑制率也增加。另外,姜黃素也抑制Hep-2細胞的侵襲和轉(zhuǎn)移能力,隨著藥物濃度的增加,其抑制作用也增強。 3、經(jīng)姜黃素作用后Hep-2細胞中FoxM1mRNA水平較作用前明顯下降,差異有統(tǒng)計學意義(P0.05)。 結(jié)論: 1、在喉癌中FoxM1蛋白表達水平的增加可能與喉癌的發(fā)生、浸潤轉(zhuǎn)移密切相關(guān)。 2、姜黃素能抑制喉癌細胞的增殖、侵襲、轉(zhuǎn)移能力,其中的機制可能與抑制FoxM1表達相關(guān)。
[Abstract]:Objective: 1. To study the expression and clinical significance of FoxM1 in laryngeal carcinoma. To study the effect of curcumin on the proliferation, invasion and metastasis of laryngeal carcinoma cell line Hep-2. To provide a new strategy for the treatment of laryngeal cancer. 3. To study the expression changes of FoxM1 before and after curcumin treatment in laryngeal carcinoma cell line Hep-2, and to explore the role of FoxM1 in the treatment of laryngeal carcinoma cell line Hep-2. Methods: 1. The expression of FoxM1 protein in 89 cases of laryngeal carcinoma and 89 cases of adjacent laryngeal papilloma was detected by immunohistochemical method. Their expression in different pathological tissues and their relationship with the clinicopathological parameters of laryngeal carcinoma were analyzed. 2. MTT assay was used. Invasion assay and migration assay were used to detect the proliferation of laryngeal carcinoma cell line Hep-2 after curcumin treatment in vitro. 3. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of FoxM1 in laryngeal cancer cells before and after curcumin treatment. Results: 1 the positive rate of FoxM1 protein expression in laryngeal carcinoma, laryngeal papilloma and adjacent tissues decreased gradually. The difference was statistically significant (P0.05). The expression of FoxM1 protein decreased with the increase of tissue differentiation (P0.05), increased with the increase of T stage (P0.05). The expression of FoxM1 protein in the group with lymph node metastasis was higher than that in the group without lymph node metastasis (P0.05), and it was expressed with the increase of clinical stage. 2. Curcumin could inhibit the proliferation of laryngeal carcinoma cell line Hep-2 in vitro. The inhibitory rate of curcumin on Hep-2 cell proliferation was also increased with the increase of curcumin concentration and time. In addition, curcumin also inhibited the invasion and metastasis of Hep-2 cells. With the increase of drug concentration, the inhibitory effect of curcumin on Hep-2 cells was also enhanced. The expression of FoxM1 mRNA in Hep-2 cells decreased significantly after curcumin treatment. The difference was statistically significant (P0.05). Conclusion: 1. The increase of FoxM1 protein expression in laryngeal carcinoma may be closely related to the occurrence, invasion and metastasis of laryngeal carcinoma. Curcumin can inhibit the proliferation, invasion and metastasis of laryngeal carcinoma cells. The mechanism may be related to the inhibition of FoxM1 expression.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R739.65

【參考文獻】

相關(guān)期刊論文 前3條

1 楊甫文;黃金中;林曉嵐;甄澤年;陳賢明;;姜黃素對鼻咽癌細胞增殖的抑制作用[J];中國耳鼻咽喉頭頸外科;2006年03期

2 張麗娟;陸茵;;姜黃素抗腫瘤機制研究進展[J];中國中醫(yī)藥信息雜志;2008年04期

3 安繼紅;陳鵬;辛秀芹;;姜黃素體外抗腫瘤作用的觀察[J];實用臨床醫(yī)藥雜志;2007年03期

，

本文編號：2082132