本文選題：間充質(zhì)干細(xì)胞 + 視神經(jīng)損傷　； 參考：《青島大學(xué)》2011年碩士論文

【摘要】：目的觀察間充質(zhì)干細(xì)胞治療視神經(jīng)損傷的安全性和療效。方法以MEM/12培養(yǎng)基,采用淋巴細(xì)胞分離液間接分離法,分離間充質(zhì)干細(xì)胞(MSCs),以巾壁培養(yǎng)法進(jìn)行原代培養(yǎng)和純化。倒置顯微鏡下觀察生長(zhǎng)狀態(tài)和形態(tài)學(xué)變化,共聚焦顯微鏡下觀察細(xì)胞表面抗原CD34,CD44、CD29免疫組化染色的鑒定,分離獲得間充質(zhì)干細(xì)胞；搜集2010年12月至2011年9月來(lái)我院住院的視神經(jīng)損傷患者23人,其中男15人,女8人,年齡在5-77歲之間,平均年齡30.1歲,其中原發(fā)性視神經(jīng)萎縮12人,遺傳性Lebers病5人,視神經(jīng)發(fā)育不良者1人,視隔發(fā)育不良者2人,視網(wǎng)膜色素變性者3人。治療前后進(jìn)行各項(xiàng)指標(biāo)檢測(cè),包括谷丙轉(zhuǎn)氨酶、谷草轉(zhuǎn)氨酶、尿素氮、肌酐、血糖、甘油三酯、膽固醇、乙肝表面抗原、凝血酶原時(shí)間、部分凝血酶原時(shí)間、甲胎球蛋白、癌胚抗原檢查,眼科檢查視力、眼壓、測(cè)杯盤比等,采用經(jīng)分離獲得的干細(xì)胞對(duì)收集的23位視神經(jīng)損傷患者分四個(gè)階段進(jìn)行治療,每次給藥間隔3-5天,每次給予2-4*107個(gè)干細(xì)胞。對(duì)視神經(jīng)損傷患者治療前后檢測(cè)閃光型視覺(jué)誘發(fā)電位的進(jìn)行對(duì)比,分析間充質(zhì)干細(xì)胞對(duì)視神經(jīng)損傷的治療效果。結(jié)果熒光顯微鏡下可見(jiàn)均強(qiáng)烈表達(dá)CD44,而CD34無(wú)表達(dá)。表明MSCs表達(dá)間充質(zhì)細(xì)胞表面抗原而不表達(dá)造血細(xì)胞、祖細(xì)胞及內(nèi)皮細(xì)胞表面抗原,這與骨髓來(lái)源的MSCs免疫組化結(jié)果一致。干細(xì)胞治療后后實(shí)驗(yàn)室指標(biāo)、眼壓、測(cè)杯盤比無(wú)顯著性差異,視力、閃光型視覺(jué)誘發(fā)電位的潛時(shí)、波幅變化明顯。結(jié)論成功獲取間充質(zhì)干細(xì)胞；間充質(zhì)干細(xì)胞移植治療視神經(jīng)損傷患者的過(guò)程安全可靠；間充質(zhì)干細(xì)胞移植治療視神經(jīng)損傷患者視力、閃光型視覺(jué)誘發(fā)電位均有改善。
[Abstract]:Objective To observe the safety and efficacy of mesenchymal stem cells (MSCs) in the treatment of optic nerve injury. Methods the MEM/12 culture medium was used to isolate mesenchymal stem cells (MSCs) by indirect separation of lymphocyte separation solution. Detection of cell surface antigen CD34, CD44, CD29 immunohistochemical staining and isolation of mesenchymal stem cells; 23 patients with optic nerve injury hospitalized in our hospital from December 2010 to September 2011 were collected, including 15 men and 8 women, aged 5-77 years, with an average age of 30.1 years, including 12 of primary optic atrophy and 5 hereditary Lebers disease. 1 people with optic nerve dysplasia, 2 with dysplasia and 3 of retinitis pigmentosa, were tested before and after treatment, including glutamic aminotransferase, glutamic aminotransferase, urea nitrogen, creatinine, glycemia, triglyceride, cholesterol, HBsAg, prothrombin time, partial prothrombin time, fetal globulin, and carcinoembryonic antigen Examination, ophthalmological examination of visual acuity, intraocular pressure, cup and disc ratio, and the use of isolated stem cells for treatment of 23 patients with optic nerve injury in four stages, each time interval 3-5 days, each time given to 2-4*107 stem cells. Comparison of flash visual evoked potentials before and after treatment of optic nerve injury patients were compared and analyzed. The effect of mesenchymal stem cells on optic nerve injury. Results the fluorescence microscope showed that CD44 was strongly expressed, but CD34 was not expressed. It showed that MSCs expressed the surface antigen of mesenchymal cells and did not express the hematopoietic cells, progenitor cells and endothelial cells surface antigen, which was in accordance with the result of MSCs immunization in bone marrow. After stem cell treatment, the experiment was carried out. There was no significant difference in ventricular index, intraocular pressure and cup and disk ratio, visual acuity and flashing visual evoked potential were obvious. Conclusion mesenchymal stem cells were successfully obtained; mesenchymal stem cell transplantation was safe and reliable for patients with optic nerve injury; mesenchymal stem cell transplantation was used to treat visual and flash vision in patients with optic nerve injury. The evoked potential was improved.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R774.6

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 修陽(yáng)暉;林發(fā)森;朱益華;;視神經(jīng)損傷后影響軸突再生的因素[J];福建醫(yī)藥雜志;2006年03期

2 崔丙周;李恩;楊波;王博;;人臍血間充質(zhì)干細(xì)胞移植治療脊髓損傷的實(shí)驗(yàn)研究[J];廣東醫(yī)學(xué);2011年03期

3 吳偉全;周光紀(jì);;胰腺干細(xì)胞的分子標(biāo)志及其獲得途徑[J];廣東醫(yī)學(xué)院學(xué)報(bào);2009年05期

4 謝琳,賀翔鴿;視神經(jīng)損傷免疫學(xué)治療的研究進(jìn)展[J];國(guó)外醫(yī)學(xué).眼科學(xué)分冊(cè);2005年01期

5 秦智勇;陸章敏;潘榮升;;基層自體角膜緣干細(xì)胞移植術(shù)治療翼狀胬肉125眼臨床觀察[J];廣西醫(yī)學(xué);2006年04期

6 吳香麗;卞小蕓;韓曉芳;;視神經(jīng)損傷后促再生因素的研究進(jìn)展[J];河北醫(yī)藥;2009年21期

7 劉濟(jì)民;;復(fù)方樟柳堿治療視神經(jīng)萎縮76例臨床觀察[J];中外醫(yī)療;2010年17期

8 李學(xué)軍;;臍帶血間充質(zhì)干細(xì)胞分離培養(yǎng)體系的優(yōu)化篩選[J];中外醫(yī)療;2010年28期

9 肖娟;陳琳;王洪美;李瑩;任玉水;黃紅云;;臨床應(yīng)用型人臍血間充質(zhì)干細(xì)胞分離培養(yǎng)與鑒定[J];解剖科學(xué)進(jìn)展;2010年04期

10 沈麗,白云,李宓,李凌松;干細(xì)胞相關(guān)名詞及其分類[J];科技術(shù)語(yǔ)研究;2003年01期

相關(guān)博士學(xué)位論文 前1條

1 李云琴;人類臍血干細(xì)胞移植對(duì)大鼠外傷性視神經(jīng)損傷的療效及其作用機(jī)制的研究[D];中南大學(xué);2009年

，

本文編號(hào)：2065457