本文選題：變應(yīng)性鼻炎 + 益生菌��； 參考：《南京醫(yī)科大學(xué)》2014年碩士論文

【摘要】：背景：雖然人們已普遍認識到攝入益生菌(probiotics)這種微生物有利于改善慢性黏膜炎癥和變態(tài)反應(yīng)性疾病,但是這類微生物如何產(chǎn)生這些免疫學(xué)上的改變?nèi)圆皇智宄?目的：對變應(yīng)性鼻炎(allergic rhinitis, AR)小鼠模型經(jīng)口途徑分別給予酶裂解的腸球菌FK-23株(lysed Enterococcus faecalis FK-23, LFK),觀測小鼠脾臟內(nèi)抗原提呈細胞(antigen-presenting cells, APC)表面組織中共刺激分子CD80和CD86的表達改變,評價益生菌對實驗性AR的免疫調(diào)節(jié)作用。 方法：共計75只BALB/c小鼠隨機分為三組：①陽性對照組；②LFK干預(yù)組；③陰性對照組。采用OVA腹腔注射及鼻部激發(fā)建立小鼠AR模型,陰性對照組小鼠采用生理鹽水代替OVA進行腹腔注射及滴鼻。用LFK菌液分別對AR小鼠模型進行灌胃,共計3周；陽性和陰性對照組以生理鹽水代替菌液灌胃處理。觀測第21天小鼠搔鼻和噴嚏次數(shù)。第22天殺鼠取材,小鼠脾臟組織用免疫組織化學(xué)法檢測APC表面共刺激分子CD80和CD86的表達。并采用酶聯(lián)免疫吸附試驗(enzyme-linked immunosorbent assay, ELISA)檢測小鼠血清中細胞因子IL-12水平。 結(jié)果：第21天小鼠搔鼻和噴嚏次數(shù),陽性對照組及LFK干預(yù)組明顯高于陰性對照組(P0.05),LFK干預(yù)組明顯低于陽性對照組(P0.05)。血清IL-12水平陽性對照組較陰性對照組顯著降低(P0.001),LFK干預(yù)組較陽性對照組顯著升高(P0.001),但與陰性對照組相比仍明顯降低(P0.001)。小鼠脾臟APC表面共刺激分子組織中CD80的表達,陽性對照組明顯低于陰性對照組及LFK干預(yù)組(P0.05),LFK干預(yù)組與陰性對照組相比差異無統(tǒng)計學(xué)意義(P0.05)；CD86的表達,陽性對照組明顯高于陰性對照組及LFK灌胃干預(yù)組(P0.05),LFK干預(yù)組與陰性對照組相比差異無統(tǒng)計學(xué)意義(P0.05)。 結(jié)論：在小鼠AR模型中,經(jīng)口途徑使用益生菌制劑品LFK可明顯減輕小鼠鼻部變應(yīng)性癥狀,改變小鼠脾臟細胞表面組織中共刺激分子CD80和CD86的表達,并對細胞因子血清IL-12水平具有一定的調(diào)節(jié)作用。
[Abstract]:Background: although it has been widely recognized that ingestion of probiotic (probiotics) is beneficial to the improvement of chronic mucosal inflammation and allergic diseases, it is not clear how these microbes produce these immunological changes. Objective: to observe the expression of costimulatory molecules CD80 and CD86 on the surface of (allergic rhinitis, cells (antigen-presenting cells) of lysed Enterococcus faecalis FK-23 (LFK). To evaluate the immunomodulatory effect of probiotics on experimental AR. Methods: a total of 75 BALB / c mice were randomly divided into three groups. The AR model of mice was established by OVA intraperitoneal injection and nasal stimulation. The mice in negative control group were treated with saline instead of OVA for intraperitoneal injection and nasal drip. The AR mouse model was perfused with LFK bacteria solution for 3 weeks, and the positive and negative control groups were treated with normal saline instead of bacteria solution. The times of nose scratching and sneezing were observed on day 21. The expression of costimulatory molecules CD80 and CD86 on the surface of APC was detected by immunohistochemical method. The level of cytokine IL-12 in serum of mice was detected by enzyme-linked immunosorbent assay (Elisa). Results: the times of nose scratching and sneezing were significantly higher in the positive control group and LFK intervention group than in the negative control group on the 21st day (P0.05) and lower in the LFK intervention group than in the positive control group (P0.05). The level of serum IL-12 in the positive control group was significantly lower than that in the negative control group (P0.001). The serum IL-12 level in LFK intervention group was significantly higher than that in the positive control group (P0.001), but it was still significantly lower than that in the negative control group (P0.001). The expression of CD80 was significantly lower in the positive control group than that in the negative control group and LFK intervention group (P0.05). There was no significant difference in CD86 expression between the LFK intervention group and the negative control group (P0.05). The positive control group was significantly higher than the negative control group and LFK gastric perfusion intervention group (P0.05) there was no significant difference between LFK intervention group and negative control group (P0.05). Conclusion: in mouse AR model, the oral administration of probiotic preparation LFK can significantly reduce the nasal allergic symptoms of mice, and change the expression of CD80 and CD86 on the surface of spleen cells in mice. And it can regulate the level of IL-12 in serum of cytokines.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R765.21

【共引文獻】

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1 張清照;]嬏飳粗，

本文編號：2058688