本文選題：重組人血管內(nèi)皮抑制素(恩度) + 放療增敏�。� 參考：《昆明醫(yī)學(xué)院》2010年碩士論文

【摘要】： 【目的】觀察血管內(nèi)皮抑制素(恩度)聯(lián)合放療對人鼻咽癌細胞系(CNE2)的增殖抑制和誘導(dǎo)細胞凋亡的作用,及其對細胞周期的影響。為血管內(nèi)皮抑制素對鼻咽癌放療增敏的可行性提供實驗依據(jù)。 【方法】四噻唑藍比色試驗(MTT)觀察不同濃度的血管內(nèi)皮抑制素對鼻咽癌CNE2濃度時間抑制作用,計算出最適濃度。四噻唑藍比色試驗(MTT)法觀察血管內(nèi)皮抑制素聯(lián)合放療對鼻咽癌CNE2時間抑制作用,以流式細胞術(shù)檢測對其細胞凋亡和周期的影響。 【結(jié)果】1.血管內(nèi)皮抑制素處理后,用倒置顯微鏡觀察恩度處理后的鼻咽癌細胞,發(fā)現(xiàn)隨藥物及作用時間的增加,CNE2癌細胞逐漸變圓浮起,折光度降低,細胞間連接松散,增殖減慢。 2.MTT法檢測不同濃度的恩度在不同時間點對CNE2的增殖抑制作用。不同濃度恩度作用于鼻咽癌細胞CNE2,隨著抑制劑作用濃度增加、作用時間的延長,腫瘤細胞存活率逐步降低。 3.單次放療劑量4Gy,恩度在濃度100mg/1的時候,綜合治療對鼻咽癌細胞CNE2的綜合作用強,綜合作用比單獨用藥或者放療作用強,隨時間的增加,腫瘤細胞的存活率逐步降低。 4.流式細胞分析顯示：人鼻咽癌細胞CNE2胞凋亡率與G2/M期百分率呈負相關(guān),恩度可能增加了細胞G2/M的放射敏感性。 5.TdT介導(dǎo)的dUTP缺口末端標記技術(shù)(TUNEL)法檢測細胞凋亡：恩度濃度為100mg/1,單次劑量4Gy,作用72h,以PI雙染色法流式細胞儀測定細胞凋亡率和壞死率,對照組細胞凋亡率僅為1.7%,細胞壞死率為8.1%,實驗組細胞凋亡率為11.6%。 【結(jié)論】1.一定濃度的血管內(nèi)皮抑制素對人鼻咽癌細胞系(CNE2)有抑制作用,作用呈時間濃度依耐性。 2.血管內(nèi)皮抑制素聯(lián)合放療對人鼻咽癌細胞系(CNE2)有增敏作用,呈時間依耐性。
[Abstract]:[objective] to observe the effects of vascular endothelial inhibitor (Endothelin) combined with radiotherapy on the proliferation and apoptosis of human nasopharyngeal carcinoma cell line (CNE2) and its effect on cell cycle. [methods] MTT assay was used to observe the time inhibitory effect of different concentrations of vascular endothelin on CNE2 concentration in nasopharyngeal carcinoma (NPC). The optimum concentration is calculated. MTT assay was used to observe the inhibitory effect of vascular endothelial inhibitor combined with radiotherapy on CNE2 of nasopharyngeal carcinoma, and the effect of flow cytometry on apoptosis and cell cycle of nasopharyngeal carcinoma. [results] 1. After treatment with vascular endothelin, nasopharyngeal carcinoma cells treated with Endosterone were observed by inverted microscope. With the increase of drug and time of action, CNE2 cancer cells gradually became round and floating, the diopter decreased, and the intercellular junctions were loosely connected. 2. MTT assay was used to detect the inhibitory effect of different concentrations of Endox on CNE2 at different time points. The survival rate of nasopharyngeal carcinoma cell line CNE _ 2 decreased gradually with the increase of the concentration of inhibitor and the prolongation of the action time. When the dose of single dose of radiotherapy was 4 Gy, the comprehensive effect of combined therapy on nasopharyngeal carcinoma cell line CNE2 was stronger than that of single dose of radiotherapy or radiotherapy at the concentration of 100mg/1, and the effect increased with time. The survival rate of tumor cells decreased gradually. 4. 4. Flow cytometry showed that the apoptosis rate of CNE2 cells was negatively correlated with the percentage of G _ 2 / M phase. TDT mediated dUTP Nick end labeling (Tunel) assay was used to detect apoptosis: Endol concentration was 100 mg / 1, single dose was 4 Gy, contact time was 72 h, flow fine flow was detected by Pi double staining. Cell apoptosis rate and necrosis rate were measured by cell analyzer. In the control group, the apoptosis rate was only 1.7%, the necrosis rate was 8.1%, and the apoptotic rate in the experimental group was 11.6%. [conclusion] 1. A certain concentration of vascular endothelin inhibited human nasopharyngeal carcinoma (CNE2) cell line (CNE2), the effect showed time concentration dependent tolerance. Endotheliostatin combined with radiotherapy can enhance the sensitivity of human nasopharyngeal carcinoma cell line (CNE2) with time dependent tolerance.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.63

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