本文選題：PCR法 + 慢性結(jié)膜炎�。� 參考：《延邊大學(xué)》2011年碩士論文

【摘要】：目的：對慢性結(jié)膜炎患者結(jié)膜囊內(nèi)分泌物進(jìn)行DNA提取并加入細(xì)菌及腺病毒特異性引物,通過PCR擴(kuò)增,探討PCR檢測應(yīng)用于慢性結(jié)膜炎病因診斷中的臨床意義。 方法：選擇慢性感染性結(jié)膜炎患者36人(36眼),慢性免疫性結(jié)膜炎患者24人(24眼),正常人22人(22眼),兩眼共患結(jié)膜炎者取較重的一側(cè)。所有受試者結(jié)膜囊分泌物提取的DNA分成兩部分,分別加入細(xì)菌引物及腺病毒引物進(jìn)行兩次PCR擴(kuò)增,凝膠電泳后在凝膠成像分析系統(tǒng)中觀察結(jié)果。對于第一組檢測人群同時進(jìn)行結(jié)膜囊細(xì)菌培養(yǎng)觀察結(jié)果。 結(jié)果：1.慢性感染性結(jié)膜炎組(Ⅰ)有14眼擴(kuò)增出520bp大小條帶,陽性率38.89%；4眼擴(kuò)增出1004bp條帶,陽性率11.11%,兩者比較有顯著性差異(χ2=7.40,P0.05)；2.慢性免疫性結(jié)膜炎組(Ⅱ)有3眼擴(kuò)增出520bp大小條帶,陽性率12.5%；1眼擴(kuò)增出1004bp條帶,陽性率4.13%。3.慢性感染性結(jié)膜炎組(Ⅰ)細(xì)菌培養(yǎng)法有19眼培養(yǎng)出陽性,陽性率為52.78%與PCR檢測法比較無顯著性差異(χ2=3.2,P0.05)。 結(jié)論：1.慢性感染性結(jié)膜炎檢測細(xì)菌感染多于腺病毒感染。2.部分慢性免疫性結(jié)膜炎患者可能對細(xì)菌或病毒成分或產(chǎn)物致敏,或者檢測出細(xì)菌為存在于結(jié)膜囊內(nèi)的正常菌群。3.雖然PCR檢測法陽性檢出率較細(xì)菌培養(yǎng)法無明顯差異,但對樣本要求不高,且經(jīng)濟(jì)又時間短,對慢性結(jié)膜炎診斷具有良好的應(yīng)用前景,是一種可供選擇的方法。
[Abstract]:Objective: to study the clinical significance of PCR detection in the etiological diagnosis of chronic conjunctivitis by extracting DNA from conjunctival sac secretions of chronic conjunctivitis and adding specific primers of bacteria and adenovirus. Methods: 36 patients with chronic infectious conjunctivitis, 24 patients with chronic immune conjunctivitis and 22 patients with normal conjunctivitis were selected. The DNA extracted from conjunctival sac secretions was divided into two parts. Bacterial primer and adenovirus primer were added for two PCR amplification. The results were observed in the gel imaging analysis system after gel electrophoresis. The results of bacterial culture of conjunctival sac were observed in the first group. The result is 1: 1. In chronic infectious conjunctivitis group (鈪，

本文編號：2037158