本文選題：IgA腎病 + 扁桃體��； 參考：《華中科技大學(xué)》2011年碩士論文

【摘要】：目的:探討L-選擇素,VLA-4α,LFA-1,ICAM-1,VCAM-1在IgA腎病(IgA nephropathy IgAN)患者與非腎炎慢性扁桃體炎患者扁桃體的表達(dá)及意義。 方法:收集10例伴有慢性扁桃體炎的IgAN患者扁桃體(IgAN組),這些患者經(jīng)腎活檢診斷為IgAN,10例不伴IgAN慢性扁桃體炎(Chronic tonsillitis CT組)作為對(duì)照。應(yīng)用Western Blot技術(shù)定量分析兩組扁桃體組織L-選擇素,VLA-4α,LFA-1,ICAM-1,VCAM-1的表達(dá),同時(shí)采用免疫組織化學(xué)技術(shù)檢測(cè)扁桃體L-選擇素,VLA-4α,LFA-1表達(dá),觀察ICAM-1,VCAM-1在生發(fā)中心和濾泡間區(qū)高內(nèi)皮小靜脈(High endothelial venules HEV)分布。采用SPSS13.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果:1.Western blot技術(shù)顯示IgAN組及CT組扁桃體L-選擇素,VLA-4α,LFA-1, ICAM-1,VCAM-1的表達(dá)分別為〔(1.10±0.09;0.80±0.14),(0.39±0.05;0.24±0.04), ( 0.41±0.06;0.29±0.06),(1.36±0..04;1.18±0.09), ( 2.56±0.63;1.60±0.32)〕。2.免疫組織化學(xué)檢測(cè)兩組各歸巢受體表達(dá)差別有顯著統(tǒng)計(jì)學(xué)意義,IgAN扁桃體生發(fā)中心和濾泡外區(qū)HEV內(nèi)血管地址素表達(dá)增強(qiáng)。 結(jié)論:扁桃體淋巴細(xì)胞歸巢異常是伴有慢性扁桃體炎的IgAN患者的重要特征,IgAN的發(fā)病機(jī)制與之有密切關(guān)聯(lián)。
[Abstract]:Objective: to investigate the expression and significance of L- selectin VLA-4 偽 in the tonsils of patients with IgA nephropathy and chronic tonsillitis. Methods: ten patients with chronic tonsillitis and 10 patients with chronic tonsillitis were collected. Ten patients with chronic tonsillitis without IgAN were diagnosed by renal biopsy as control group. The expression of L- selectin (VLA-4 偽) and ICAM-1 (VCAM-1) in tonsils were quantitatively analyzed by Western blot, and the expression of L- selectin VLA-4 偽 -lFA-1 in tonsils was detected by immunohistochemical technique. The distribution of ICAM-1 VCAM-1 in high endothelial venules was observed in the germinal center and interfollicular area. Results 1.Western blot technique showed that the expression of L- selectin in tonsils of IgAN group and CT group was 1.10 鹵0.09 鹵0.80 鹵0.140.24 鹵0.04 鹵0.04 鹵0.04 鹵1.18 鹵0.09, (2.56 鹵0.631.60 鹵0.32), (0.41 鹵0.06 鹵0.29 鹵0.06) 鹵1.18 鹵0.09, (2.56 鹵0.631.60 鹵0.32), respectively. 2. The expression of VCAM-1 in the tonsils of IgAN group and CT group was 1.18 鹵0.09, (2.56 鹵0.631.60 鹵0.32), 0.39 鹵0.050.24 鹵0.04, (0.41 鹵0.06, 0.29 鹵0.06, 1.18 鹵0.09, 2.56 鹵0.63, 1.60 鹵0.32, respectively). The difference of homing receptor expression between the two groups was statistically significant. Conclusion: the homing abnormality of tonsil lymphocytes is accompanied by slow homing in IgAN tonsil germinal center and extrafollicular area of HEV. An important feature of IgAN patients with tonsillitis is the pathogenesis of IgAN.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R692.3;R766.18

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本文編號(hào)：2003267