本文選題：年青人白內(nèi)障 + 晶狀體上皮細(xì)胞�。� 參考：《大連醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的：本研究應(yīng)用免疫組織化學(xué)SP法染色,檢測(cè)凋亡相關(guān)基因bax在年青人白內(nèi)障患者(年齡在40-55歲之間)晶狀體上皮細(xì)胞中的表達(dá)及表達(dá)程度,以探討晶狀體上皮細(xì)胞的凋亡與年青人白內(nèi)障發(fā)生的關(guān)系。 方法：采用鏈霉菌親生物素蛋白—過氧化物酶標(biāo)免疫組織化學(xué)法(steptavidin-peroxidase,SP)染色,光學(xué)顯微鏡下檢測(cè)18例年青人白內(nèi)障患者(實(shí)驗(yàn)組)和22例老年性白內(nèi)障患者(對(duì)照組)的晶狀體上皮細(xì)胞的bax基因的表達(dá)及表達(dá)程度。 結(jié)果：在低齡組年青人白內(nèi)障患者的晶狀體前囊膜上皮細(xì)胞胞漿中可見棕黃色顆粒均勻分布,提示bax基因在年青人白內(nèi)障患者的晶狀體上皮細(xì)胞中有表達(dá),其表達(dá)程度：bax基因在18例年青人白內(nèi)障患者的晶狀體上皮細(xì)胞中強(qiáng)陽性表達(dá)10例,陽性表達(dá)6例,弱陽性表達(dá)2例,無陰性表達(dá)病例；在高齡組老年性白內(nèi)障患者的晶狀體前囊膜上皮細(xì)胞胞漿中可見棕黃色顆粒均勻分布,提示bax基因在高齡老年性白內(nèi)障患者晶狀體上皮細(xì)胞中有表達(dá),其表達(dá)程度：bax基因在22例高齡白內(nèi)障患者晶狀體上皮細(xì)胞中強(qiáng)陽性表達(dá)12例,陽性表達(dá)7例,弱陽性表達(dá)3例,無陰性表達(dá)病例。凋亡相關(guān)基因bax在低齡和高齡組白內(nèi)障患者的晶狀體上皮細(xì)胞中均有明顯的表達(dá),且兩組間bax基因的表達(dá)沒有顯著性差異(P0.05)。 結(jié)論：年青人白內(nèi)障(40-55歲)與老年性白內(nèi)障(60-80歲)發(fā)病的最初誘因可能不同,但最終都因晶狀體上皮細(xì)胞的凋亡而導(dǎo)致白內(nèi)障的發(fā)生。
[Abstract]:Objective: to detect the expression and expression of apoptosis-related gene bax in lens epithelial cells of young cataract patients (aged 40-55 years) by immunohistochemical SP staining. To investigate the relationship between lens epithelial cell apoptosis and cataract in young people. Methods: Streptomyces biophile protein and peroxidase labeled immunohistochemical method was used for the staining of Streptomyces vidin-peroxidase (SPX). The expression and degree of bax gene in lens epithelial cells of 18 young cataract patients (experimental group) and 22 senile cataract patients (control group) were detected under optical microscope. Results: there were brown granules in the cytoplasm of lens anterior capsule epithelial cells in young cataract patients, suggesting that bax gene was expressed in lens epithelial cells of young cataract patients. There were 10 cases of strong positive expression, 6 cases of positive expression, 2 cases of weak positive expression, and no case of negative expression. The distribution of brown granules in the cytoplasm of lens anterior capsule epithelial cells in the elderly patients with senile cataract showed that bax gene was expressed in lens epithelial cells of senile cataract patients. There were 12 cases of strong positive expression, 7 cases of positive expression, 3 cases of weak positive expression and no negative expression in 22 cases of senile cataract. The expression of apoptosis-related gene bax in lens epithelial cells of both young and aged cataract patients was significant, and there was no significant difference in the expression of bax gene between the two groups (P 0.05). Conclusion: the initial inducement of cataract in young people aged 40-55 years may be different from that in senile cataract patients (60-80 years old), but eventually it is caused by the apoptosis of lens epithelial cells.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R776.1
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本文編號(hào)：1969507