本文選題：脂質(zhì)體槲皮素 + 糖尿病��； 參考：《青島大學(xué)》2010年碩士論文

【摘要】： 目的觀察脂質(zhì)體槲皮素(Liposomal Quercertin, LQ)對糖尿病(diabetes mellitus,DM)大鼠視網(wǎng)膜糖基化終產(chǎn)物(advanced glycation end products, AGEs)生成及受體(RAGE mRNA)表達(dá)的影響,并探討可能的作用機(jī)制。 方法采用蒸發(fā)-高壓均質(zhì)法制備脂質(zhì)體槲皮素懸濁液,鏈脲佐菌素(streptozocin,STZ)法誘導(dǎo)DM大鼠模型,并將DM大鼠隨機(jī)分為DM模型組、脂質(zhì)體槲皮素低、中、高劑量灌胃組(50mg/kg.d、150mg/kg.d及250mg/kg.d)及氨基胍灌胃組(100mg/kg.d),連續(xù)給藥12周；實(shí)驗(yàn)結(jié)束時(shí),HE染色觀察視網(wǎng)膜形態(tài)學(xué)變化,免疫熒光法、RT-PCR方法分別檢測DM大鼠視網(wǎng)膜中AGEs的沉積情況及RAGE mRNA的表達(dá),并與正常對照組比較。 結(jié)果 1.視網(wǎng)膜形態(tài)學(xué)變化：DM模型組視網(wǎng)膜各層出現(xiàn)典型的DM病理學(xué)改變,脂質(zhì)體槲皮素中劑量組視網(wǎng)膜病理學(xué)改變較DM模型組減輕。 2.脂質(zhì)體槲皮素各劑量組大鼠體重較DM模型組無明顯變化,血糖較DM模型組略有下降,但差異無統(tǒng)計(jì)學(xué)意義。 3.較DM模型組結(jié)果(AGEs:0.922±0.005、RAGE mRNA:1.192±0.098)比較,脂質(zhì)體槲皮素各組及氨基胍組視網(wǎng)膜中AGEs沉積減少,RAGEmRNA表達(dá)也降低(P0.05),以脂質(zhì)體槲皮素中劑量組(AGEs:0.734±0.007、RAGE mRNA:0.724±0.028)降低最為明顯(P0.01)。 結(jié)論 1.脂質(zhì)體槲皮素能夠在一定程度上減輕視網(wǎng)膜形態(tài)學(xué)病理改變,改善STZ性DM大鼠一般狀況,可能有降血糖的作用,尚需進(jìn)一步實(shí)驗(yàn)證實(shí)。 2.脂質(zhì)體槲皮素可通過有效減少DM大鼠視網(wǎng)膜中AGEs的沉積,下調(diào)RAGE mRNA的表達(dá),即通過抑制非酶糖基化的形成,早期干預(yù)視網(wǎng)膜微血管病變的發(fā)生。 3.本研究為脂質(zhì)體槲皮素的下一步臨床應(yīng)用研究打下基礎(chǔ),同時(shí)也對探索DM的臨床中藥治療提供新的思路和理論依據(jù)。
[Abstract]:Objective to investigate the effects of liposomal Quercerin (LQQ) on the production of advanced glycation end products, AGEs) and the expression of receptor rage mRNAs in retina of diabetic rats with diabetes mellitus Mellitus (DMN), and to explore the possible mechanism. Methods Liposome quercetin suspension was prepared by evaporation-high pressure homogenization method, streptozocin STZ (streptozocin STZ) method was used to induce DM rat model, and DM rats were randomly divided into DM model group with low and moderate quercetin liposome. 50 mg / kg 路d and 250 mg / kg 路d in high dose gavage group and 100 mg / kg 路d ~ (-1) aminoguanidine group respectively for 12 weeks. At the end of the experiment, morphological changes of retina were observed by HE staining, and AGEs deposition and RAGE mRNA expression in retina of DM rats were detected by immunofluorescence RT-PCR. And compared with the normal control group. Result 1. The pathological changes of retina in the middle dose of liposome quercetin group were less than those in the DM model group. 2. There was no significant change in body weight and blood glucose in each dose of liposome quercetin group as compared with DM group, but the difference was not statistically significant. 3. Compared with DM model group, ages: 0.922 鹵0.005 rage mRNA:1.192 鹵0.098), the deposition of AGEs in retina of quercetin group and aminoguanidine group was also decreased (P 0.05), especially in the middle dose group of liposome quercetin 0.734 鹵0.007 rage mRNA:0.724 鹵0.028). Conclusion 1. The liposome quercetin can alleviate the pathological changes of retinal morphology and improve the general condition of STZ diabetic rats to a certain extent, which may have the effect of hypoglycemia, which needs further experimental verification. 2. Liposome quercetin can effectively reduce the deposition of AGEs and down-regulate the expression of RAGE mRNA in the retina of DM rats, that is, by inhibiting the formation of non-enzymatic glycosylation, early intervention of retinal microangiopathy occurs. 3. This study laid a foundation for the further clinical application of liposome quercetin and provided new ideas and theoretical basis for exploring the clinical treatment of DM.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R774.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李文靜,顧建新,陳惠黎,朱禧星;丹參對體外葡萄糖與蛋白質(zhì)非酶結(jié)合的影響[J];上海醫(yī)科大學(xué)學(xué)報(bào);1998年02期

2 白秀燕,陳麗,鄭冬梅,侯新國;水飛薊素對糖尿病植物神經(jīng)病變的實(shí)驗(yàn)研究[J];泰山醫(yī)學(xué)院學(xué)報(bào);2001年04期

3 呂學(xué)愛,關(guān)廣聚,晉運(yùn)玲,孫云,文蓉珠,吳濤;氨基胍對糖尿病大鼠腎臟非酶糖基化的抑制作用[J];泰山醫(yī)學(xué)院學(xué)報(bào);2004年03期

4 曹群華,瞿偉菁,牛偉,鄧云霞,王意文,謝晶晶;沙棘黃酮對鏈脲佐菌素致糖尿病大鼠降糖作用[J];營養(yǎng)學(xué)報(bào);2005年02期

5 鄭里翔;STZ和alloxan聯(lián)合使用復(fù)制實(shí)驗(yàn)性糖尿病大鼠模型[J];中國病理生理雜志;1999年08期

6 嚴(yán)金川,劉乃豐;氨基胍對非酶糖基化人臍靜脈內(nèi)皮細(xì)胞信號轉(zhuǎn)導(dǎo)物DAG影響[J];高血壓雜志;1999年04期

7 蘇俊鋒,郭長江,韋京豫,楊繼軍,李云峰;槲皮素體內(nèi)外抗氧化作用的比較研究[J];中國應(yīng)用生理學(xué)雜志;2002年04期

8 段有金,王韶穎,三輪一智,奧田潤;五種中藥對蛋白質(zhì)非酶糖基化的抑制作用[J];中國糖尿病雜志;1998年04期

9 李厚麗;翟光喜;祝偉偉;李凌冰;馬玉坤;;槲皮素固體脂質(zhì)納米粒的制備及小鼠口服吸收研究[J];中國藥學(xué)雜志;2008年06期

10 傅靜奕,田浩明,梁藎忠;槲皮素對糖尿病大鼠腎臟的保護(hù)作用及機(jī)制[J];中華內(nèi)分泌代謝雜志;2000年01期

，

本文編號：1960296