發(fā)布時(shí)間：2018-05-27 17:26

  本文選題：葡萄膜黑色素瘤 + 淋巴管生成��； 參考：《眼科》2015年04期

【摘要】：目的研究葡萄膜黑色素瘤(UM)組織中腫瘤相關(guān)淋巴管生成與腫瘤組織病理學(xué)高危因素的關(guān)系。設(shè)計(jì)實(shí)驗(yàn)研究。研究對(duì)象北京同仁醫(yī)院眼科病理室2009-2014年55例(55眼)UM眼球石蠟組織標(biāo)本。方法采用淋巴管內(nèi)皮特異性標(biāo)記物L(fēng)YVE-1和D2-40,通過免疫組織化學(xué)Elivision法檢測(cè)UM組織中淋巴管生成情況,結(jié)合患者臨床組織病理學(xué)資料進(jìn)行統(tǒng)計(jì)學(xué)分析;統(tǒng)計(jì)腫瘤的微淋巴管密度(lymphatic microvessel density,LMD),結(jié)合CD-34檢測(cè)腫瘤微血管密度(microvessel density,MVD)的結(jié)果分析兩者的相關(guān)性。主要指標(biāo)UM組織的淋巴管陽(yáng)性率、LMD、MVD。結(jié)果 55例UM組織標(biāo)本中36例(65.5%)觀察到淋巴管。腫瘤累及睫狀體者淋巴管陽(yáng)性率(95.0%)明顯高于未累及者(48.6%),發(fā)生球外蔓延者淋巴管陽(yáng)性率(93.3%)明顯高于未發(fā)生者(55.0%),腫瘤最大基底直徑16 mm者淋巴管陽(yáng)性率(82.4%)明顯高于≤16 mm者(38.1%),上皮樣細(xì)胞類型者淋巴管陽(yáng)性率(88.0%)明顯高于非上皮樣細(xì)胞類型者(46.7%)(P均0.05)�；颊吣挲g(P=0.187)、性別(P=0.452)、眼別(P=0.663)、是否有虹膜新生血管(P=0.395)、是否繼發(fā)視網(wǎng)膜脫離(P=0.641)、視盤是否受累(P=0.121)、是否浸潤(rùn)鞏膜(P=0.284)及術(shù)前是否放療(P=0.483)與UM組織中的淋巴管陽(yáng)性率均無(wú)統(tǒng)計(jì)學(xué)相關(guān)。LMD與MVD成正相關(guān)(r=0.917,P0.001)。結(jié)論 2/3的葡萄膜黑色素瘤中存在腫瘤相關(guān)淋巴管,其在UM的侵襲轉(zhuǎn)移中可能起到一定的作用。免疫組織化學(xué)法檢測(cè)淋巴管生成是判斷UM患者預(yù)后的重要指標(biāo)之一。
[Abstract]:Objective to study the relationship between tumor-associated lymphangiogenesis and high risk factors of tumor histopathology in uveal melanoma (UM). Design experimental research. Participants 55 cases (55 eyes) of UM eyeball paraffin tissues from 2009 to 2014 were studied in ophthalmic pathology department of Tongren Hospital in Beijing. Methods Lymphangiogenesis in UM tissues was detected by immunohistochemical Elivision method with LYVE-1 and D2-40, and the clinicopathological data were analyzed statistically. The microlymphatic microvessel density (LMDD) and microvessel density (MVD) were measured by CD-34. Main outcome measures the positive rate of lymphatic vessels in UM tissue was higher than that of LMD-MVD. Results lymphatic vessels were observed in 36 of 55 UM tissue specimens. The positive rate of lymphatic vessels in patients with tumor involvement of ciliary body (95.0) was significantly higher than that in those without involvement (48.6%), and the positive rate of lymphatic vessels in patients with extrabulbar spread (93.33cm) was significantly higher than that in patients without ciliary body involvement (55.0%). The positive rate of lymphatic vessels in cases with a maximum basal diameter of 16 mm was significantly higher than that of 鈮，

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