本文選題：視網(wǎng)膜小膠質(zhì)細(xì)胞 + 脂多糖�。� 參考：《中南大學(xué)》2010年碩士論文

【摘要】： 目的： 探討環(huán)氧合酶-2選擇性抑制劑NS-398對(duì)脂多糖刺激下的離體培養(yǎng)的大鼠視網(wǎng)膜小膠質(zhì)細(xì)胞TNF-a和COX-2表達(dá)的調(diào)節(jié)作用。 方法： 脂多糖刺激離體培養(yǎng)的第四代SD新生大鼠視網(wǎng)膜小膠質(zhì)細(xì)胞,將培養(yǎng)細(xì)胞分為五組：A組空白對(duì)照組：實(shí)驗(yàn)組根據(jù)含不同濃度的NS-398分為：B組實(shí)驗(yàn)對(duì)照組(10μg/LLPS);C組低濃度作用組(50μmol/L NS-398+10μg/LLPS);D組中濃度作用組(100μmol/LNS-398+ 10μg/LLPS);E組高濃度作用組(200μmol/L NS-398+10μg/LLPS);分別作用24及48小時(shí)后,RT-PCR半定量檢測(cè)各組中TNF-a和COX-2的表達(dá),并進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果： 1.視網(wǎng)膜小膠質(zhì)細(xì)胞分別作用24h及48h后,各組間TNF-a的mRNA表達(dá)量的差異有統(tǒng)計(jì)學(xué)意義(P0.05),隨培養(yǎng)液中NS-398濃度的增加,LPS刺激后視網(wǎng)膜小膠質(zhì)細(xì)胞中TNF-a的mRNA的表達(dá)量逐漸降低。同組視網(wǎng)膜小膠質(zhì)細(xì)胞在不同時(shí)間的TNF-a的mRNA表達(dá)量除A組(空白對(duì)照組)差異無(wú)統(tǒng)計(jì)學(xué)意義外(P0.05),余差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 2.視網(wǎng)膜小膠質(zhì)細(xì)胞分別作用24h及48h后,各組間COX-2的mRNA表達(dá)量的差異有統(tǒng)計(jì)學(xué)意義(P0.05),隨培養(yǎng)液中NS-398濃度的增加,LPS刺激后視網(wǎng)膜小膠質(zhì)細(xì)胞中COX-2的mRNA的表達(dá)量逐漸降低。同組視網(wǎng)膜小膠質(zhì)細(xì)胞在不同時(shí)間的COX-2的mRNA表達(dá)量除A組(空白對(duì)照組)差異無(wú)統(tǒng)計(jì)學(xué)意義外(P0.05),余差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論： 1.視網(wǎng)膜小膠質(zhì)細(xì)胞可以被激活釋放炎癥相關(guān)的細(xì)胞因子TNF-a和COX-2,引起一系列的炎癥反應(yīng)。 2.環(huán)氧合酶-2選擇性抑制劑NS-398抑制視網(wǎng)膜小膠質(zhì)細(xì)胞表達(dá)TNF-a和COX-2,繼而抑制炎癥反應(yīng)。 3.NS-398對(duì)視網(wǎng)膜小膠質(zhì)細(xì)胞的抑制作用呈時(shí)間劑量依賴型。
[Abstract]:Objective: To investigate the effects of cyclooxygenase-2 selective inhibitor (NS-398) on the expression of TNF-a and COX-2 in cultured rat retinal microglia stimulated by lipopolysaccharide (LPS). Methods: Lipopolysaccharide stimulated retinal microglia of the fourth generation SD neonatal rats in vitro. The cultured cells were divided into five groups: control group: the experimental group was divided into two groups according to different concentration of NS-398: the control group was divided into two groups: the control group (n = 10 渭 g / L / L) treated with 10 渭 g / L LPSN / C, the control group (n = 50 渭 mol/L NS-398, n = 10 渭 g / L / L / L) and the middle concentration group (n = 100 渭 mol/LNS-398 = 10 渭 g / L / L / L / L / L / L) respectively, and the control group (n = 100 渭 mol/LNS-398 = 10 渭 g / L / L / L) were divided into two groups (n = 10). After 24 and 48 hours of exposure, semi-quantitative RT-PCR was used to detect the expression of TNF-a and COX-2 in each group. Statistical analysis was carried out. Results: 1. After treated with TNF-a for 24 and 48 hours, the expression of mRNA in retinal microglia was significantly different (P 0.05), and the expression of TNF-a mRNA in retinal microglia decreased gradually with the increase of NS-398 concentration in culture medium. The mRNA expression of retinal microglia at different time in the same group was significantly higher than that in group A (P 0.05) except that in group A (blank control group). 2. After treated with COX-2 for 24 and 48 hours, the expression of mRNA in retinal microglia was significantly different (P 0.05), and the expression of COX-2 mRNA in retinal microglia decreased gradually with the increase of NS-398 concentration in culture medium. The mRNA expression of retinal microglia at different time in the same group was significantly higher than that in group A (P 0.05) except that in group A (blank control group). Conclusion: 1. Retinal microglia can be activated to release inflammatory cytokines TNF-a and COX-2, causing a series of inflammatory responses. 2. Cyclooxygenase-2 selective inhibitor NS-398 inhibits the expression of TNF-a and COX-2 in retinal microglia and then inhibits inflammation. The inhibitory effect of 3.NS-398 on retinal microglia was time-and-dose-dependent.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R587.2;R774.1

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