本文選題：Wistar大鼠 + 激光凝固術(shù)��； 參考：《川北醫(yī)學(xué)院》2011年碩士論文

【摘要】：目的:探討532-二極管激光建立大鼠青光眼模型眼壓的變化規(guī)律。 方法:分別選用健康2月齡和4月齡Wistar大鼠各40只,每組光凝30只,其余10只作空白對(duì)照觀測(cè)眼壓。光凝一眼,另一眼為自身對(duì)照,左右眼交替。每7天光凝一次,共4次,滿1月時(shí)存活的大鼠隨機(jī)選擇一半作為實(shí)驗(yàn)組,和空白對(duì)照組同時(shí)在全麻下取出雙眼眼球;另一半為繼續(xù)觀察組,再繼續(xù)觀測(cè)眼壓1月,滿2月時(shí)在全麻下取出雙眼眼球。光凝方法:采用532-二極管激光經(jīng)角膜緣光凝小梁網(wǎng)360°,激光參數(shù)設(shè)置為0.2s/0.5w,每只眼光凝約150個(gè)點(diǎn)。分別于光凝前、光凝后即刻及光凝后每天同一時(shí)間段用Tonolab眼壓計(jì)測(cè)量大鼠雙眼眼壓,每只眼連續(xù)測(cè)量6次,取平均值,同時(shí)觀察雙眼前節(jié)情況。 結(jié)果:2月齡大鼠第一次光凝前平均眼壓為15±3.8mmHg,光凝后即刻平均眼壓為24±6.3mmHg,差異有統(tǒng)計(jì)學(xué)意義(t=6.632,P0.001);4月齡大鼠第一次光凝前平均眼壓為13±2.8mmHg,光凝后即刻平均眼壓為26±10.8mmHg,差異有統(tǒng)計(jì)學(xué)意義(t=6.485, P0.001);光凝后即刻2月齡和4月齡大鼠眼壓峰值分別為60mmHg和54mmHg。兩組大鼠光凝后即刻平均眼壓差異無統(tǒng)計(jì)學(xué)意義(t=-1.623, P0.05)。光凝后第1天平均眼壓最高然后逐日下降,在第6天恢復(fù)到正常范圍。在光凝后6天觀測(cè)中,2月齡和4月齡眼壓峰值分別為66 mmHg和74mmHg。兩組大鼠每次重復(fù)光凝后眼壓均有上述相同的變化。2月齡第一次光凝后即刻眼壓升高的動(dòng)物為63%,4月齡為60%。眼壓升高動(dòng)物最多出現(xiàn)在光凝后第1天,2月齡可達(dá)到77%,4月齡可達(dá)到88%,均出現(xiàn)在第四次光凝后的第1天。大多數(shù)動(dòng)物眼壓在光凝后第6天恢復(fù)到正常范圍。 結(jié)論:532-二極管激光經(jīng)角膜緣光凝小梁網(wǎng)建立的大鼠青光眼模型有可能是一過性眼壓升高,大鼠慢性青光眼動(dòng)物模型可能需反復(fù)多次光凝小梁網(wǎng)才能獲得。
[Abstract]:Objective: to investigate the changes of intraocular pressure (IOP) in rat model of glaucoma induced by 532-diode laser. Methods: 40 healthy Wistar rats aged 2 months and 4 months were selected, 30 rats in each group were photocoagulated, the other 10 rats were used as blank control to observe intraocular pressure (IOP). Light coagulation, another eye for self-control, left and right eyes alternately. Once every 7 days, 4 times a day, half of the rats who survived at the end of one month were randomly selected as the experimental group, and the eyes of both eyes were taken out under general anesthesia with the blank control group. The other half was the continuous observation group, and then the IOP was observed for 1 month. Take out your eyes under general anesthesia for two months. The method of photocoagulation: using 532-diode laser through the limbal photocoagulation trabecular net 360 擄, the laser parameter is set to 0.2 s / 0.5 w.Each eye sets about 150 points. The intraocular pressure (IOP) was measured by Tonolab intraocular pressure meter at the same time of day before photocoagulation, immediately after photocoagulation and at the same time after photocoagulation. The intraocular pressure of each eye was measured for 6 consecutive times, and the mean value was taken, and the anterior segment of the eyes was observed at the same time. Results the mean IOP was 15 鹵3.8 mmHg before the first photocoagulation and 24 鹵6.3 mmHg immediately after photocoagulation in 2 month old rats. The difference was statistically significant (13 鹵2.8 mmHgand 26 鹵10.8mmHg respectively). The peak IOP of 2-month and 4-month-old rats was 60mmHg and 54mm Hg, respectively. There was no significant difference in IOP between the two groups immediately after photocoagulation (P 0.05). The mean IOP was highest on the 1st day after photocoagulation, then decreased daily, and returned to normal on the 6th day. The peak values of IOP at 2 and 4 months of age were 66 mmHg and 74 mm Hg, respectively, 6 days after photocoagulation. The intraocular pressure (IOP) of the rats in the two groups increased immediately after photocoagulation for the first time at the age of 2 months, and the IOP was 60 months old at the age of 63 to 4 months after repeated photocoagulation. The elevation of intraocular pressure (IOP) occurred mostly on the 1st day and 2nd month after photocoagulation, and the age of 77 and 4 months could reach 888.All of them appeared on the first day after the fourth photocoagulation. IOP in most animals returns to normal on the 6th day after photocoagulation. Conclusion the rat model of glaucoma induced by 532 diode laser through the limbal photocoagulation trabecular reticulum may be a transient elevation of intraocular pressure, and the animal model of chronic glaucoma may be obtained by repeated photocoagulation of trabecular meshwork.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R775

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