本文選題：裸鼠 + 喉癌細(xì)胞　； 參考：《中國(guó)醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 實(shí)驗(yàn)?zāi)康?觀察裸鼠喉癌細(xì)胞系Hep-2移植瘤的成瘤率,繪制腫瘤生長(zhǎng)曲線,用免疫組化方法觀察移植瘤中STK15基因的表達(dá)情況。 實(shí)驗(yàn)方法 將符合條件的17只nu/nu純系裸小鼠隨機(jī)分成兩組,實(shí)驗(yàn)組10只,對(duì)照組7只。實(shí)驗(yàn)組每只裸鼠右側(cè)前肢腋下皮下注射0.2mlHep-2細(xì)胞懸液,直至皮下形成0.5cm大小皮丘。對(duì)照組每只裸鼠相同部位注射0.2ml生理鹽水,直至皮下形成0.5cm大小皮丘,定期觀察兩組裸鼠接種部位液體的吸收情況,計(jì)算成瘤率,繪制成瘤曲線。5周后處死實(shí)驗(yàn)動(dòng)物,剝離腫瘤,用免疫組化方法觀察移植瘤中STK15基因的表達(dá)情況。 實(shí)驗(yàn)結(jié)果 實(shí)驗(yàn)組裸鼠成瘤率100%,免疫組化可證實(shí)裸鼠喉癌細(xì)胞系Hep-2移植瘤中STK15過表達(dá)。 實(shí)驗(yàn)結(jié)論 通過動(dòng)物實(shí)驗(yàn)證實(shí)STK15基因是喉癌的發(fā)生發(fā)展的原因之一。
[Abstract]:experimental purpose



The tumor growth curve was observed and the expression of STK15 gene in transplanted tumor was observed by immunohistochemistry .



experimental method



Two groups of nude mice were randomly divided into two groups , the experimental group was 10 rats and the control group was 7 . The experimental group injected 0.2 ml of normal saline at the same location on the right anterior limb of the nude mice , and then injected 0.2ml normal saline at the same site of the nude mice . The tumor rate was calculated and the tumor rate was calculated . After 5 weeks , the experimental animals were sacrificed and the tumor was stripped . The expression of STK15 gene was observed by immunohistochemistry .



experimental results



The tumor rate of nude mice in experimental group was 100 % , and the expression of STK15 was confirmed by immunohistochemistry .



experimental conclusion



Animal experiments prove that the STK15 gene is one of the causes of the development of laryngeal cancer .

【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R739.65

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相關(guān)期刊論文 前4條

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本文編號(hào)：1864301