本文選題：tom40 + tom20　； 參考：《華中科技大學(xué)》2011年碩士論文

【摘要】：目的:探討線粒體蛋白輸入功能變化在老年性聾發(fā)病機(jī)制中的作用,為老年性聾的預(yù)防及治療提供新的思路。 方法:96只1月齡雌性Wistar大鼠隨機(jī)分為A、B、C、D四組,每組24只。其中,B、C、D組為實(shí)驗(yàn)組,A組為空白對(duì)照組,分別給予每日頸部皮下注射D-半乳糖150mg/kg,300mg/kg,500mg/kg和等量生理鹽水,共8周。聽(tīng)性腦干反應(yīng)(ABR)檢測(cè)4組大鼠給藥前后聽(tīng)閾。實(shí)時(shí)熒光定量PCR檢測(cè)給藥后各組大鼠內(nèi)耳組織中線粒體DNA 4834 bp缺失突變率。實(shí)時(shí)熒光定量RT-PCR檢測(cè)給藥后各組大鼠內(nèi)耳中tom40,tom20,tim23 mRNA水平的表達(dá)變化。Western blot法檢測(cè)給藥后各組大鼠內(nèi)耳中tom40,tom20,tim23蛋白水平的表達(dá)變化。 結(jié)果:四組間給藥前后ABR聽(tīng)閾提高值無(wú)明顯差異(p0.05)。線粒體DNA 4834 bp缺失突變率在各組分別為:A組3.91%±0.33%,B組9.16%±0.32%,C組11.65%±0.58%,D組15.09%±0.94%,與A組相比,B,C,D三組D-半乳糖劑量組大鼠內(nèi)耳組織中CD缺失率均顯著升高(p0.05) ,且B,C,D各不同劑量組間,CD缺失率均有顯著性差異(p0.05)。tom40,tom20,tim23 mRNA水平的表達(dá)在各組間無(wú)明顯差異(p0.05)。tom40,tom20蛋白水平的表達(dá)除A、B兩組間無(wú)明顯差異外(p0.05),其余各組間差異均有統(tǒng)計(jì)學(xué)意義,且隨半乳糖劑量的增大顯著降低(p0.05),tim23蛋白水平的表達(dá)在各組間無(wú)明顯差異(p0.05)。 結(jié)論:tom40,tom20蛋白表達(dá)水平在擬老化大鼠內(nèi)耳組織中存在著不同程度的降低,提示在內(nèi)耳組織老化的發(fā)生過(guò)程中線粒體蛋白輸入轉(zhuǎn)運(yùn)系統(tǒng)可能廣泛受累,其中tom40和tom20的降低較早出現(xiàn)并且較為嚴(yán)重,會(huì)直接影響TOM復(fù)合體功能,并進(jìn)一步引起核編碼線粒體蛋白輸入障礙。
[Abstract]:Objective: to explore the role of mitochondrial protein input function in the pathogenesis of presbycusis and to provide a new idea for the prevention and treatment of presbycusis. Methods one month old female Wistar rats were randomly divided into four groups, 24 rats in each group. Group D was treated by subcutaneous injection of D-galactose (150mg / kg) and saline (300mg / kg) and saline for 8 weeks (n = 8), and the control group (n = 10) was given subcutaneous injection of D-galactose (150 mg / kg) and normal saline (n = 500 mg / kg) for 8 weeks. Auditory brainstem response (ABR) was used to detect the hearing threshold before and after administration. The deletion rate of mitochondrial DNA 4834 BP in the inner ear of rats was detected by real-time fluorescence quantitative PCR. Real-time fluorescence quantitative RT-PCR was used to detect the expression of tom40 tom20tim23 mRNA in the inner ear of rats in each group. Western blot was used to detect the expression of tom40 tom20tim23 protein in the inner ear of each group. Results: there was no significant difference in ABR hearing threshold between the four groups before and after administration. The deletion rate of mitochondrial DNA 4834 BP was 3.91% 鹵0.33 in group B and 9.16% 鹵0.32 in group B respectively. The deletion rate of CD in group D was 15.09% 鹵0.94% in group D, which was significantly higher than that in group A (P 0.05). In addition, there was no significant difference in the expression of p0.05N. Tom40m20tim23 mRNA between the two groups. The expression of p0.05n.tom40tom20 protein was not significantly different between the two groups, except that there was no significant difference between the two groups (P 0.05), and there was significant difference between the other groups, and there was no significant difference between the two groups in the expression of p0. 05%, p0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, respectively. With the increase of galactose dose, the expression of p0.05 titim23 protein was significantly decreased in each group. There was no significant difference in p0.05 protein expression among the three groups. Conclusion the level of the protein expression in the inner ear of the aged rats is decreased to some extent, which suggests that the mitochondrial protein input transport system may be involved extensively during the aging of the inner ear tissue, and it is suggested that the mitochondrial protein input transport system may be involved extensively during the aging process of the inner ear tissue. The decrease of tom40 and tom20 appeared earlier and more serious, which directly affected the function of TOM complex and further caused the obstruction of nuclear coding mitochondrial protein input.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R764.43

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本文編號(hào)：1839039