本文選題：IL- + 葡萄膜炎�。� 參考：《免疫學(xué)雜志》2015年07期

【摘要】：目的探討IL-17參與急性神經(jīng)炎癥的神經(jīng)保護作用與機制。方法用光感受器間維生素A類結(jié)合蛋白(interphotoreceptor retinoid binding protein,IRBP)免疫純系Lewis大鼠,建立急性-單向型實驗性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)模型。RT-PCR法檢測在EAU進展中效應(yīng)T細胞相關(guān)分子的表達,免疫熒光法檢測視網(wǎng)膜內(nèi)膠質(zhì)纖維酸性蛋白(glial fibrillary acidic portein,GFAP)的表達。以PMA/Ionomycin刺激大鼠外周血單個核細胞產(chǎn)生的炎性上清模擬含高水平IL-17的炎性環(huán)境,ELISA法檢測IL-17等細胞因子水平,Tunel法檢測細胞凋亡。結(jié)果 EAU發(fā)病過程中視網(wǎng)膜局部呈現(xiàn)含高水平IL-17的炎性環(huán)境,其中星型膠質(zhì)細胞高度活化。以炎性上清刺激可誘發(fā)原代培養(yǎng)的小腦神經(jīng)元大量凋亡,加入活化后星型膠質(zhì)細胞或上清則使凋亡顯著降低。IL-17中和抗體可顯著抑制炎性上清對神經(jīng)元的保護作用。結(jié)論急性神經(jīng)炎癥中星型膠質(zhì)細胞通過分泌IL-17發(fā)揮神經(jīng)保護作用。
[Abstract]:Objective to investigate the neuroprotective effect and mechanism of IL-17 in acute neuritis. Methods the immunized pure Lewis rats were immunized with interphotoreceptor retinoid binding protein (IRBP), an acute unidirectional experimental autoimmune uveitis model. RT-PCR was used to detect the expression of effector T cell-related molecules in the progression of EAU. The expression of glial fibrillary acidic in retina was detected by immunofluorescence. The inflammatory supernatant produced by peripheral blood mononuclear cells (PBMC) stimulated by PMA/Ionomycin was used to simulate the inflammatory environment with high level of IL-17. The level of cytokines such as IL-17 was detected by Elisa and apoptosis was detected by Tunel method. Results the inflammatory environment with high level of IL-17 was present in the retina during the course of EAU, in which astrocytes were highly activated. The stimulation of inflammatory supernatant could induce a large number of apoptosis of primary cultured cerebellar neurons, and the addition of activated astrocytes or supernatants could significantly reduce the apoptosis. IL-17 neutralizing antibody could significantly inhibit the protective effect of inflammatory supernatant on neurons. Conclusion astrocytes play a neuroprotective role in acute neuritis by secreting IL-17.
【作者單位】： 齊齊哈爾醫(yī)學(xué)院免疫教研室;
【基金】：齊齊哈爾市科技局社會發(fā)展項目(SFGG-201209)
【分類號】：R774.6

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