本文選題：曲安奈德 + 血管內(nèi)皮細(xì)胞生長因子��； 參考：《復(fù)旦大學(xué)》2011年碩士論文

【摘要】：第一部分氧誘導(dǎo)視網(wǎng)膜病變(oxygen-induced retinopathy, OIR)模型的建立 目的建立OIR模型小鼠。方法7日齡C57BL/6J新生小鼠6只作為單純給氧組,置于氧濃度75%的容器內(nèi)連續(xù)飼養(yǎng)5天至小鼠12日齡,再置于正�？諝猸h(huán)境下飼養(yǎng)至17日齡；6只同齡新生小鼠在正�？諝猸h(huán)境飼養(yǎng)至17日齡作為單純對照組。采用FFA眼底造影、視網(wǎng)膜鋪片ADP染色及計數(shù)眼球切片中突破視網(wǎng)膜內(nèi)界膜新生血管的內(nèi)皮細(xì)胞核數(shù)等方法觀察視網(wǎng)膜新生血管情況。結(jié)果單純給氧組FFA檢查可見眼底視網(wǎng)膜新生血管生成,視網(wǎng)膜鋪片ADP酶染色見視網(wǎng)膜血管大片無灌注區(qū),血管部分閉塞,分支減少,分布較紊亂；而單純對照組小鼠視網(wǎng)膜血管分布均勻規(guī)則,無閉塞。單純給氧組平均每個眼球切片中突破視網(wǎng)膜內(nèi)界膜的內(nèi)皮細(xì)胞核數(shù)為(24.8±7.1)個,較對照組顯著增多(P0.05)。結(jié)論該小鼠模型具有血管無灌注區(qū)、血管閉塞、新生血管形成等改變,重復(fù)性好、可定量研究,是研究視網(wǎng)膜新生血管比較合適的動物模型。 第二部分曲安奈德(triamcinolone acetonide, TA)抑制視網(wǎng)膜新生血管的機(jī)制研究 目的通過OIR模型小鼠,探討TA抑制視網(wǎng)膜新生血管的可能機(jī)制。方法7日齡C57BL/6J新生小鼠36只,共分為4組。其中18只制作OIR動物模型,并且于12日齡時玻璃體腔內(nèi)注射曲安奈德2μl,作為高氧TA組；對側(cè)眼注射相同體積的平衡鹽溶液(balanced salt solution, BSS),作為高氧BSS組；其余兩組小鼠在正常環(huán)境下飼養(yǎng),余處理同前,分別作為正常BSS組和正常TA組。用FFA造影及二磷酸腺苷(adenosine diphos-phate, ADP)酶法染色,了解視網(wǎng)膜血管情況；用視網(wǎng)膜切片HE染色,計數(shù)小鼠視網(wǎng)膜新生血管內(nèi)皮細(xì)胞核數(shù),加以比較；用免疫組織化學(xué)法檢測各組小鼠視網(wǎng)膜切片VEGF、SDF-1、CD14含量。用Realtime PCR檢測VEGF、SDF-1 mRNA的表達(dá)。結(jié)果與高氧BSS組相比,高氧TA組突破視網(wǎng)膜內(nèi)界膜內(nèi)皮細(xì)胞細(xì)胞核數(shù)目明顯減少(P0.05),血管分布更規(guī)則,FFA造影接近正常眼底。SDF-1、VEGF mRNA及SDF-1、VEGF和CD14蛋白表達(dá)在高氧TA組低于高氧BSS組(P0.05),正常TA組和正常BSS組之間無明顯差異(P0.05)。VEGF、SDF-1與CD14的IOD/AOI值均呈顯著線性相關(guān)(偏相關(guān)分析P0.05)。結(jié)論TA能有效抑制小鼠視網(wǎng)膜新生血管形成,其機(jī)制可能是通過減少單核/巨噬細(xì)胞聚集進(jìn)而降低SDF-1及VEGF的表達(dá)而實現(xiàn)。
[Abstract]:The first part is the establishment of oxygen-induced retinopathy (OIRs) model. Objective to establish OIR model mice. Methods six 7-day-old C57BL/6J newborn mice were fed in a 75% oxygen concentration container for 5 days to 12 days of age. Six newborn mice of the same age were reared to 17 days of age in normal air as the control group. The retinal neovascularization was observed by FFA fundus angiography, ADP staining and counting the number of endothelial nuclei of neovascularization through the inner limiting membrane of the retina. Results the retinal neovascularization was observed in the oxygenated group by FFA. The retinal ADP enzyme staining showed that there was no perfusion area, partial occlusion, less branches and more disordered distribution of retinal vessels in the oxygenated group. In the control group, the retinal blood vessels were distributed uniformly and without occlusion. The number of endothelial nuclei breaking through the inner retinal membrane was 24.8 鹵7.1 in the oxygen alone group, which was significantly higher than that in the control group (P 0.05). Conclusion the mouse model has no perfusion area, vascular occlusion, angiogenesis and so on. It has good reproducibility and can be quantitatively studied. It is a suitable animal model for the study of retinal neovascularization. The mechanism of triamcinolone acetonide (TA) in inhibiting retinal neovascularization Objective to explore the possible mechanism of TA inhibiting retinal neovascularization in OIR mice. Methods 36 7-day-old C57BL/6J mice were divided into 4 groups. Eighteen OIR animal models were made and triamcinolone acetonide (2 渭 l) was injected intravitreally as hyperoxia TA group at 12 days of age, and balanced salt solution in the same volume was injected into the contralateral eye as hyperoxia BSS group. The other two groups of mice were fed in normal environment. The remaining mice were treated as normal BSS group and normal TA group respectively. The retinal vessels were studied by FFA and adenosine diphos-phosphate enzyme staining, and the number of endothelial nuclei of retinal neovascularization in mice were counted by HE staining in retina sections, and the number of endothelial nuclei of retinal neovascularization in mice was compared. Immunohistochemical method was used to detect the content of CD14 in retina sections of mice. The expression of VEGFG SDF-1 mRNA was detected by Realtime PCR. Results compared with hyperoxia BSS group, In hyperoxia TA group, the number of endothelial cell nucleus of retinal inner boundary was significantly reduced, and the expression of VEGF mRNA and SDF-1 mRNA and CD14 protein in hyperoxia TA group was significantly lower than that in hyperoxia TA group compared with that in hyperoxia BSS group, while in normal TA group and positive group, the expression of VEGF-VEGF and CD14 in hyperoxia TA group was more regular than that in hyperoxia TA group. There was no significant difference between normal BSS group and normal BSS group. There was a significant linear correlation between IOD/AOI value of CD14 and SDF-1 (partial correlation analysis P 0.05). Conclusion TA can effectively inhibit the formation of retinal neovascularization in mice, which may be achieved by reducing mononuclear / macrophage aggregation and thus the expression of SDF-1 and VEGF.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R774.1

【共引文獻(xiàn)】

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5 吉桂芳;“視覺2020”行動與上海市防盲治盲工作探討[J];上海預(yù)防醫(yī)學(xué)雜志;2005年07期

6 邵靜儷;沈桃娟;;上海市靜安寺街道老年人盲和低視力現(xiàn)況調(diào)查[J];上海預(yù)防醫(yī)學(xué)雜志;2008年01期

7 劉X;許迅;;糖尿病性視網(wǎng)膜病變眼科治療的新進(jìn)展[J];上海醫(yī)學(xué);2009年05期

8 朱劍鋒;王寧;許迅;鄒海東;王偉偉;吳美芳;;糖尿病患者盲與低視力的流行病學(xué)調(diào)查[J];實用防盲技術(shù);2006年01期

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10 陶雁亭;李健民;曹怡;吳偉豪;李影;俞正娟;;上海浦東三林鎮(zhèn)老年人盲和低視力流行病學(xué)調(diào)查[J];實用防盲技術(shù);2007年03期

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