本文選題：喉鱗狀細胞癌 + BUB1B��； 參考：《中國醫(yī)科大學》2010年碩士論文

【摘要】： 目的 通過檢測BUB1B mRNA在喉鱗狀細胞癌及其相鄰正常喉黏膜組織中的表達,探討B(tài)UB1B基因在喉鱗狀細胞癌發(fā)生發(fā)展中的作用及其臨床意義。 材料與方法 一、標本的來源與制備 所選30例喉鱗癌標本均取自中國醫(yī)科大學附屬第一醫(yī)院耳鼻咽喉科2009年11月至2010年2月手術(shù)切除標本,所有患者術(shù)前均未行放療和化療,均無遠隔器官轉(zhuǎn)移,術(shù)后病理示其切緣皆為陰性。每例分別取新鮮癌組織及癌旁1 cm以外非癌喉黏膜組織-70℃凍存?zhèn)溆谩?二、實驗方法 提取總RNA,反轉(zhuǎn)錄合成cDNA,以β-actin為內(nèi)參照,應(yīng)用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)方法檢測30例喉鱗狀細胞癌組織及相鄰癌旁正常黏膜組織中BUBIB mRNA表達情況。 結(jié)果 以BUBIB和β-act in的光密度值之比做為BUB1B mRNA表達的相對值,半定量分析BUB1B mRNA在喉鱗狀細胞癌組織和相鄰正常喉黏膜組織中的表達。喉鱗狀細胞癌組織中BUBIB/β-actin為0.215±0.076,癌旁正常喉黏膜組織為0.317±0.128,經(jīng)統(tǒng)計學分析(配對t檢驗),2組數(shù)據(jù)差異具有統(tǒng)計學意義,(t=4.521,P0.05),采用獨立樣本t檢驗的方法,對其在臨床分型、組織學分組及淋巴結(jié)轉(zhuǎn)移等方面進行統(tǒng)計學分析,其表達在不同臨床分期中有差異,而在不同臨床分型、組織學分級及淋巴結(jié)轉(zhuǎn)移轉(zhuǎn)移中無明顯差異(P＞O.05)。 BUB1B mRNA在喉癌組織中低表達,且其低表達可能與喉癌的發(fā)生發(fā)展有關(guān)。
[Abstract]:objective
By detecting the expression of BUB1B mRNA in laryngeal squamous cell carcinoma and its adjacent normal laryngeal mucosa, the role of BUB1B gene in the development of laryngeal squamous cell carcinoma and its clinical significance were investigated.
Materials and methods
First, the source and preparation of the specimen
All the 30 specimens of laryngeal squamous cell carcinoma were taken from the surgical excision specimens from the Department of Otolaryngology, the First Affiliated Hospital of China Medical University, from November 2009 to February 2010. None of the patients were treated with radiotherapy and chemotherapy before operation. No distant organ metastasis was performed. The membrane tissue is stored at -70 C for storage.
Two, experimental method
The total RNA, reverse transcriptional synthesis of cDNA and beta -actin as internal reference were used to detect the expression of BUBIB mRNA in 30 cases of laryngeal squamous cell carcinoma and adjacent normal mucosa tissues by reverse transcription polymerase chain reaction.
Result
Using the ratio of BUBIB and beta -act in as the relative value of BUB1B mRNA expression, the expression of BUB1B mRNA in the tissues of laryngeal squamous cell carcinoma and adjacent normal laryngeal mucosa was semi quantified. The BUBIB/ beta -actin in the squamous cell carcinoma of the larynx was 0.215 + 0.076, and the normal laryngeal mucosa adjacent to the carcinoma was 0.317 + 0.128, and was statistically analyzed (paired t). The difference between the 2 groups was statistically significant, (t=4.521, P0.05). The methods of independent sample t test were used to analyze the clinical classification, histologic grouping and lymph node metastasis, and the expression was different in different clinical stages, but in different clinical classification, histology classification and lymph node metastasis. Significant differences (P > O.05).
The expression of BUB1B mRNA is low in laryngeal carcinoma, and its low expression may be related to the development of laryngeal carcinoma.

【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R739.65

【參考文獻】

相關(guān)期刊論文 前2條

1 李英慧;中心體異常與腫瘤發(fā)生的相關(guān)性[J];國外醫(yī)學.遺傳學分冊;2002年03期

2 李英慧,李福才,王曦,趙旭,葉燕,孫興和,孫開來;喉癌STK15基因異常與中心體擴增的研究[J];中華醫(yī)學遺傳學雜志;2004年03期

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本文編號：1775335