本文選題：喉癌　切入點(diǎn)：腫瘤血管生成　出處：《山西醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的 探討caveolin-1和VEGF在喉癌組織中表達(dá)水平與兩者在喉癌腫瘤血管生成中作用、相互關(guān)系及臨床意義。 對(duì)象標(biāo)本源自山西醫(yī)科大學(xué)第一臨床附屬醫(yī)院耳鼻喉科2008年8月到2010年9月40例喉鱗癌標(biāo)本，其中20例不伴頸淋巴結(jié)轉(zhuǎn)移喉癌標(biāo)本，20例伴有頸淋巴結(jié)轉(zhuǎn)移喉癌標(biāo)本，記錄每位患者的詳細(xì)臨床資料，正常對(duì)照是8例正常喉粘膜組織。 方法 1.選取40例喉癌組織及8例正常喉粘膜組織，應(yīng)用caveolin-1抗體、CD34單克隆抗體及VEGF單克隆抗體，采用免疫組織化學(xué)技術(shù)檢查caveolin-1、CD34、VEGF蛋白的表達(dá)。 2.選取40例喉癌組織及8例正常喉粘膜組織，用逆轉(zhuǎn)錄PCR(RT-PCR)法研究caveolin-1mRNA和VEGFmRNA的表達(dá)。 結(jié)果 1．Caveolin-1蛋白主要表達(dá)于細(xì)胞胞膜和胞漿中，血管內(nèi)皮也可見表達(dá)，正常組織陽(yáng)性率75.0%，不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組陽(yáng)性率25.0%，伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組為65.0%，caveolin-1mRNA相對(duì)灰度值分別為0.701±0.073，0.350±0.050和0.572±0.061，，不論從基因水平還是蛋白水平三組之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.05），兩兩比較亦均有顯著性差異（P＜0.05），伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組caveolin-1表達(dá)明顯高于不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組（P㩳0.05），caveolin-1表達(dá)與頸淋巴轉(zhuǎn)移呈顯著相關(guān)。 2．VEGF表達(dá)于腫瘤細(xì)胞的胞漿中，正常組織陽(yáng)性率25%，不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組陽(yáng)性率55%，伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組為75%，VEGFmRNA相對(duì)灰度值分別為0.614±0.321、0.801±0.473和1.413±0.586，不論從基因水平還是蛋白水平三組之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.05），兩兩比較亦均有顯著性差異（P＜0.05），伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組VEGF表達(dá)明顯高于不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組（P＜0.05），VEGF表達(dá)與頸淋巴轉(zhuǎn)移呈顯著相關(guān)。 3．正常組織、不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組和伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組內(nèi)CD34標(biāo)記MVD分別為3.42±1.15、21.34±4.62和30.19±2.60，三組之間差異有統(tǒng)計(jì)學(xué)意義（P＜0.05），兩兩比較亦均有顯著性差異（P＜0.05）。MVD與VEGF之間呈明顯正相關(guān)（r=0.635，P0.01），40例喉癌組織中MVD與caveolin-1之間呈明顯相關(guān)（r=0.424，P0.05）。 4．從蛋白和基因水平，正常組和不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組之間caveolin-1與VEGF表達(dá)呈負(fù)相關(guān)（P＜0.05），不伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組和伴有頸淋巴結(jié)轉(zhuǎn)移的喉癌組之間caveolin-1與VEGF表達(dá)呈正相關(guān)（P＜0.05）。 結(jié)論 喉癌細(xì)胞可以合成、分泌大量VEGF，其在腫瘤血管生成中起促進(jìn)作用，腫瘤微血管密度是評(píng)價(jià)腫瘤血管生成的重要指標(biāo)。研究顯示caveolin-1在正常喉組織中表達(dá)較高，正常組織轉(zhuǎn)化為喉癌時(shí)表達(dá)降低，并與微血管密度成負(fù)相關(guān)，提示caveolin-1抑制腫瘤血管的生成，其機(jī)制可能與抑制VEGF表達(dá)相關(guān)；caveolin-1在伴頸淋巴轉(zhuǎn)移喉癌組織中表達(dá)水平較無(wú)頸淋巴轉(zhuǎn)移喉癌組織高，且與微血管密度呈正相關(guān)，提示caveolin-1促進(jìn)腫瘤血管生成，其機(jī)制可能與caveolin-1變異或促進(jìn)VEGF表達(dá)相關(guān)，進(jìn)而促進(jìn)腫瘤侵襲和轉(zhuǎn)移。
[Abstract]:objective
To investigate the expression level of caveolin-1 and VEGF in laryngeal carcinoma and their role in the angiogenesis of laryngeal cancer, and the relationship and clinical significance.
The object samples from the first clinical hospital of Shanxi Medical University from August 2008 to September 2010 in Department of ENT, 40 cases of laryngeal squamous cell carcinoma, 20 cases with cervical lymph node metastasis in laryngeal carcinoma, 20 cases with cervical lymph node metastasis in laryngeal carcinoma, record the detailed clinical data of each patient, the control is 8 cases of normal laryngeal mucosa.
Method
1. 40 cases of laryngeal carcinoma and 8 cases of normal laryngeal mucosa tissues were selected. The expression of caveolin-1, CD34 and VEGF protein was detected by immunohistochemistry, using caveolin-1 antibody, CD34 monoclonal antibody and VEGF monoclonal antibody.
2. the expression of caveolin-1mRNA and VEGFmRNA in 40 cases of laryngeal carcinoma and 8 cases of normal laryngeal mucosa were studied by reverse transcriptase PCR (RT-PCR).
Result
1.Caveolin-1 protein was mainly expressed in the cell membrane and in cytoplasm of vascular endothelial expression of normal tissue is also visible, the positive rate was 75%, without cervical lymph node metastasis in laryngeal carcinoma group, the positive rate was 25%, with cervical lymph node metastasis in laryngeal carcinoma group was 65%, caveolin-1mRNA relative gray value of 0.701 + 0.073,0.350 + 0.050 and 0.572 + 0.061, there was statistical significance from the gene level and protein level differences between the three groups (P < 0.05), 22 were also significantly different (P < 0.05), with lymph node metastasis in laryngeal carcinoma group caveolin-1 expression was significantly higher than without lymph node metastasis in laryngeal carcinoma group (P? 0.05), caveolin-1 the expression was significantly correlated with lymph node metastasis.
2.VEGF expressed in cytoplasm of tumor cells in normal tissues, the positive rate was 25%, without cervical lymph node metastasis in laryngeal carcinoma group, the positive rate was 55%, with cervical lymph node metastasis in laryngeal carcinoma group was 75%, the relative gray scale values of VEGFmRNA were 0.614 + 0.321,0.801 + 0.473 and 1.413 + 0.586, no matter from the gene level or protein level between the three groups was statistically significant (P < 0.05), 22 were also significantly different (P < 0.05), the expression was significantly higher than that associated with cervical lymph node metastasis in laryngeal carcinoma group VEGF laryngeal carcinoma group with the cervical lymph node metastasis (P < 0.05), the expression of VEGF was significantly correlated with lymph node metastasis.
3. normal tissues, without cervical lymph node metastasis in laryngeal carcinoma group with cervical lymph node metastasis in laryngeal carcinoma group CD34 labeled MVD were 3.42 + 1.15,21.34 + 4.62 and 30.19 + 2.60, there were statistically significant differences between the three groups (P < 0.05), 22 were also significantly different (P < 0.05) is a significant positive correlation between.MVD and VEGF (r=0.635, P0.01), showed a significant correlation between MVD and caveolin-1 in 40 cases of laryngeal carcinoma tissues (r=0.424, P0.05).
4. from the level of gene and protein, the normal group and the group without metastasis of laryngeal carcinoma between caveolin-1 and VEGF expression was negatively related to cervical lymph node (P < 0.05), not associated with cervical lymph node metastasis in laryngeal carcinoma group with cervical lymph node metastasis in laryngeal carcinoma group between caveolin-1 and VEGF expression of Cheng Zhengxiang (P < 0.05).
conclusion
Laryngeal cancer cells can synthesis and secretion of VEGF, which play an important role in tumor angiogenesis, tumor microvessel density is an important index for evaluation of tumor angiogenesis. Research shows that high levels of caveolin-1 in normal laryngeal tissues, normal tissue into laryngeal carcinoma expression and microvessel density decreased, and a negative correlation that generate caveolin-1 inhibition of tumor angiogenesis and its mechanism may be related to the inhibition of VEGF expression; caveolin-1 with cervical lymph node metastasis in laryngeal carcinoma tissue expression was no lymph node metastasis in laryngeal carcinoma is high, and the correlation with microvessel density positively, suggesting that caveolin-1 promote tumor angiogenesis, and its mechanism may be related to caveolin-1 mutation or promote the expression of VEGF, and promote the invasion and metastasis of tumor.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.65

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本文編號(hào)：1708408