本文選題：消旋山莨菪堿　切入點(diǎn)：豚鼠　出處：《復(fù)旦大學(xué)》2011年碩士論文

【摘要】：第一部分10%消旋山莨菪堿對豚鼠形覺剝奪性近視的作用和安全性 目的：探討10%消旋山莨菪堿對豚鼠形覺剝奪性近視的作用以及安全性。方法：2-3周齡英國短毛花色豚鼠30只,隨機(jī)分為3組,每組10只,采用右眼遮蓋半透膜建造形覺剝奪模型,左眼為自身對照,A組形覺剝奪+10%消旋山莨菪堿20μl玻璃體腔注射1次,B組形覺剝奪+生理鹽水20μl玻璃體腔注射1次,C組單純形覺剝奪組。所有動物飼養(yǎng)于5001ux白光照明環(huán)境中,照明周期為12h：12h。干預(yù)前后用A超測量眼軸、前房深度和晶體厚度,算出玻璃體腔長度；帶狀光檢影法測量屈光度；曲率計(jì)測量角膜曲率半徑；右眼與左眼的差異作為評價(jià)指標(biāo)以消除飼養(yǎng)過程中的組間差異。2周后實(shí)驗(yàn)結(jié)束每組隨機(jī)選取2只豚鼠處死后立即摘取眼球,行HE染色觀察眼部組織結(jié)構(gòu)。SPSS軟件行統(tǒng)計(jì)分析,以P0.05為統(tǒng)計(jì)學(xué)差異。 結(jié)果：1、各組基線測量左右眼眼軸、前房深度、晶體厚度、玻璃體腔長度、屈光度、角膜曲率均無統(tǒng)計(jì)學(xué)差異(P0.05)。2、干預(yù)后2周復(fù)測,給藥組左右眼眼軸和玻璃體腔差異明顯小于對照組(P0.05),玻璃體腔長度差異與眼軸長度差異顯著相關(guān)(r=0.904,P0.001)。各組左右眼屈光度差異、曲率半徑差異、晶體厚度差異、前房深度差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3、HE染色后顯微鏡下觀察給藥組角膜、睫狀體、視網(wǎng)膜、鞏膜均未見毒性改變。 結(jié)論：10%消旋山莨菪堿可抑制豚鼠形覺剝奪性近視的眼軸增長,主要抑制玻璃體腔長度。10%消旋山莨菪堿玻腔注射未發(fā)現(xiàn)組織學(xué)毒性改變。 目的：探討10%消旋山莨菪堿玻腔注射對豚鼠形覺剝奪性近視眼球后極部鞏膜TGF-β2和MMP-2蛋白表達(dá)的影響。 方法：2-3周齡英國短毛花色豚鼠18只,隨機(jī)分為3組,每組6只,采用右眼遮蓋半透膜建造形覺剝奪模型,左眼為自身對照,A組形覺剝奪+10%消旋山莨菪堿20μl玻璃體腔注射1次,B組單純形覺剝奪組,C組正�？瞻讓φ战M。所有動物飼養(yǎng)于5001ux白光照明環(huán)境中,照明周期為12h：12h。2周后處死豚鼠取右眼后極部鞏膜組織,采用蛋白印跡法檢測MMP-2蛋白和TGF-β2蛋白含量。 結(jié)果：鞏膜MMP-2蛋白表達(dá)從高到低依次為：形覺剝奪組,10%消旋山莨菪堿組,正常對照組。鞏膜TGF-β2表達(dá)從從高到低依次為：正常對照組,10%消旋山莨菪堿組,形覺剝奪組。 結(jié)論：10%消旋山莨菪堿玻腔注射可使形覺剝奪后豚鼠鞏膜蛋白表達(dá)發(fā)生變化；10%消旋山莨菪堿抑制MMP-2蛋白表達(dá),調(diào)高TGF-β2蛋白表達(dá)；這一作用是否參與干預(yù)鞏膜重塑過程,并對近視進(jìn)展產(chǎn)生影響,值得進(jìn)一步研究。
[Abstract]:Effect and safety of 10% racemic anisodamine on form-deprivation myopia in guinea pigsObjective: to investigate the effect and safety of 10% racemic anisodamine on form-deprivation myopia in guinea pigs.Methods Thirty British short-haired pigmented guinea pigs aged 2-3 weeks were randomly divided into 3 groups with 10 guinea pigs in each group. The model of shape deprivation was constructed by covering the right eye with semi-permeable membrane.The left eye in group A was deprived of 10% racemic anisodamine 20 渭 l intravitreal injection. Group B was injected with normal saline 20 渭 l once in group C and group C was deprived of simple form perception.All the animals were raised in 5001ux white lighting environment, and the illumination period was 12 h: 12 h.Before and after intervention, the length of vitreous cavity was calculated by measuring the depth of eye axis, anterior chamber depth and lens thickness by A-ultrasound, the diopter was measured by zonal photogrammetry, the radius of curvature of cornea was measured by curvature meter.The difference between right eye and left eye was used as evaluation index to eliminate the difference between groups during feeding. 2 weeks later, two guinea pigs in each group were randomly selected to take out the eyeball immediately after death, and the eye tissue structure was observed by HE staining. SPSS software was used for statistical analysis.P0.05 as the statistical difference.Results: there was no significant difference in eye axis, anterior chamber depth, lens thickness, length of vitreous cavity, diopter and corneal curvature in all groups.The difference of eye axis and vitreous cavity in the treatment group was significantly lower than that in the control group (P 0.05), and there was a significant correlation between the length of the vitreous cavity and the length of the eye axis.The difference of diopter, radius of curvature, thickness of lens and depth of anterior chamber were not statistically significant in each group. No toxic changes were found in cornea, ciliary body, retina and sclera after HE staining.Conclusion 10% racemic anisodamine can inhibit the axial growth of form-deprived myopia in guinea pigs, and no histological toxicity was found after vitreous injection of 10% racemic anisodamine.Aim: to investigate the effect of 10% racemic anisodamine injection on the expression of TGF- 尾 2 and MMP-2 protein in posterior sclera of form-deprived myopia in guinea pigs.Methods Eighteen British short-haired pigmented guinea pigs aged 2-3 weeks were randomly divided into 3 groups (6 in each group). The model of shape deprivation was constructed by covering the right eye with semi-permeable membrane.The left eye was given 10% racemic anisodamine 20 渭 l intravitreal injection into the vitreous cavity of the control group A (n = 10). Group B (n = 10) was injected once into the vitreous cavity of group B, and group C (n = 10) was normal blank control group.All the animals were raised in the white light environment of 5001ux. After 12h:12h.2 week, the guinea pigs were killed to take the sclera tissue of the posterior pole of the right eye. The contents of MMP-2 protein and TGF- 尾 2 protein were detected by Western blotting.Results: the expression of MMP-2 protein in sclera from high to low was 10% racemic anisodamine group and normal control group.The order of TGF- 尾 2 expression in sclera from high to low was: normal control group, 10% racemic anisodamine group, form deprivation group.Conclusion injection of 10% racemic anisodamine into vitreous cavity can change the scleral protein expression of guinea pigs after form deprivation. 10% racemic anisodamine can inhibit the expression of MMP-2 protein and increase the expression of TGF- 尾 2 protein, and whether this effect is involved in the process of scleral remodeling.It has an effect on myopia progression and is worthy of further study.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R778.11

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 牟達(dá);何芳;;TGF-β與MAPK細(xì)胞內(nèi)信號轉(zhuǎn)導(dǎo)通路的交互調(diào)節(jié)及其在心血管疾病中的作用[J];國際病理科學(xué)與臨床雜志;2006年02期

2 劉雙珍,朱玉廣,吳小影,李鳳云,閔曉珊,許惠卓;TGF-β1在雞形覺剝奪性近視眼中的表達(dá)及作用[J];眼科研究;2005年01期

3 戴淑真;曾駿文;王麗婭;;哌侖西平對雞形覺剝奪性近視的療效及作用機(jī)制[J];中華眼科雜志;2006年01期

4 萬珍玲;胡忠良;劉保安;;TGF-β1可通過ERK信號通路調(diào)節(jié)胃癌細(xì)胞MMP-2和MMP-9的表達(dá)[J];腫瘤防治研究;2009年02期

5 蔣麗琴,呂帆,周翔天,趙海嵐,王瑞卿,瞿佳;環(huán)戊通松弛豚鼠睫狀肌的調(diào)節(jié)作用[J];眼視光學(xué)雜志;2004年04期

6 樂琦驊;褚仁遠(yuǎn);吳偉;;哌侖西平滴眼液治療豚鼠形覺剝奪性近視[J];眼視光學(xué)雜志;2006年02期

7 賈丁;周翔天;呂帆;周勝杰;陳蔚;瞿佳;;豚鼠形覺剝奪性近視眼鞏膜基質(zhì)金屬蛋白酶-2表達(dá)的實(shí)驗(yàn)研究[J];眼視光學(xué)雜志;2006年06期

8 胡萍;李鏡海;;透鏡誘導(dǎo)豚鼠近視眼鞏膜基質(zhì)中基質(zhì)金屬蛋白酶2及其組織抑制劑和生長因子的變化[J];眼視光學(xué)雜志;2008年01期

9 陳靜嫦;鄧大明;麥光煥;;消旋山莨菪堿滴眼液治療兒童近視[J];眼視光學(xué)雜志;2009年06期

10 王淑然;葉俊杰;龍琴;;負(fù)透鏡誘導(dǎo)豚鼠離焦性近視眼后部鞏膜Ⅰ型膠原、基質(zhì)金屬蛋白酶-2及金屬蛋白酶組織抑制因子-2的表達(dá)[J];中國醫(yī)學(xué)科學(xué)院學(xué)報(bào);2010年01期

相關(guān)博士學(xué)位論文 前1條

1 魏欣;視網(wǎng)膜早基因c-fos在豚鼠實(shí)驗(yàn)性近視眼中的表達(dá)及其對TH調(diào)控作用的研究[D];中南大學(xué);2006年

相關(guān)碩士學(xué)位論文 前1條

1 鄒天驥;兩性霉素B及其脂質(zhì)體治療曲霉菌性眼內(nèi)炎的對比研究[D];吉林大學(xué);2007年

，

本文編號：1688773