本文選題：喉鱗狀細胞癌　切入點：乳腺癌轉(zhuǎn)移抑制基因1　出處：《蚌埠醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：探討乳腺癌轉(zhuǎn)移抑制基因1（BRMS1）和特異AT序列結(jié)合蛋白1（SATB1）在喉鱗狀細胞癌癌組織及癌旁正常組織中的表達情況、相關(guān)性及臨床意義。 方法：1.采用免疫組化EliVision法檢測20例聲門上型喉癌和20例聲門型喉癌癌組織及相應(yīng)的癌旁正常組織中BRMS1和SATB1的表達情況。2.采用逆轉(zhuǎn)錄聚合酶鏈式反應(yīng)（RT-PCR）檢測20例聲門上型喉癌和20例聲門型喉癌癌組織及相應(yīng)的癌旁正常組織中BRMS1mRNA和SATB1mRNA的表達情況。3.分析BRMS1和SATB1在喉鱗狀細胞癌中表達的相關(guān)性及與患者的性別、年齡、臨床TNM分期、分化程度和淋巴結(jié)轉(zhuǎn)移的關(guān)系。 結(jié)果： 一.免疫組化結(jié)果 1.聲門上型喉鱗狀細胞癌癌組織中的BRMS1蛋白陽性表達低于癌旁正常組織的陽性表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 2..聲門型喉鱗狀細胞癌癌組織中的BRMS1蛋白陽性表達低于癌旁正常組織的陽性表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 3.聲門上型喉癌組織中BRMS1的蛋白陽性表達與聲門型喉癌組織中BRMS1的陽性表達的差異無統(tǒng)計學(xué)意義（P＞0.05）。 4.聲門上型喉鱗狀細胞癌癌組織中的SATB1蛋白陽性表達高于癌旁正常組織的陽性表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 5..聲門型喉鱗狀細胞癌癌組織中的SATB1蛋白陽性表達高于癌旁正常組織的陽性表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 6.聲門上型喉癌組織中SATB1蛋白的陽性表達與聲門型喉癌組織中SATB1蛋白的陽性表達差異無統(tǒng)計學(xué)意義（P＞0.05）。 7.聲門上型喉癌中，BRMS1蛋白與SATB1蛋白的表達與TNM分期、腫瘤分化程度、有無淋巴結(jié)轉(zhuǎn)移有關(guān)，與年齡、性別無關(guān)。 8.聲門型喉癌中，BRMS1蛋白與SATB1蛋白的表達與TNM分期、腫瘤分化程度有關(guān)，與年齡、性別、有無淋巴結(jié)轉(zhuǎn)移無關(guān)。 9.在聲門上型喉癌和聲門型喉癌中，BRMS1蛋白和SATB1蛋白表達均成負相關(guān)性。 二、RT-PCR結(jié)果 1.在聲門上型和聲門型組中，BRMS1mRNA在喉鱗狀細胞癌中的表達均低于癌旁正常組織的BRMS1mRNA表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 2.在聲門上型和聲門型組中，SATB1mRNA在喉鱗狀細胞癌中的表達均高于癌旁正常組織的SATB1mRNA表達，兩組差異有統(tǒng)計學(xué)意義（P＜0.05）。 結(jié)論： 1.BRMS1在聲門上型喉癌和聲門型喉癌組織中的表達水平均低于癌旁正常組織。 2.SATB1在聲門上型喉癌和聲門型喉癌組織中的表達水平均高于癌旁正常組織。 3.聲門上型喉癌中，BRMS1和SATB1的表達與TNM分期、腫瘤分化程度、淋巴結(jié)轉(zhuǎn)移有關(guān)，與患者的年齡、性別無關(guān)。 4.聲門型喉癌中，，BRMS1和SATB1的表達與TNM分期、腫瘤分化程度有關(guān)，與患者的年齡、性別、淋巴結(jié)轉(zhuǎn)移無關(guān)。 5.BRMS1和SATB1的表達呈負相關(guān)，對BRMS1和SATB1進行聯(lián)合檢測可能成為判斷喉鱗狀細胞癌淋巴結(jié)轉(zhuǎn)移情況的重要指標。
[Abstract]:Objective: to investigate the expression, correlation and clinical significance of metastasis suppressor gene 1 (BRMS1) and specific AT sequence binding protein (1) SATB1) in laryngeal squamous cell carcinoma (LSCC) and adjacent normal tissues. Methods the expression of BRMS1 and SATB1 in 20 cases of supraglottic laryngeal carcinoma and 20 cases of glottic carcinoma and their adjacent normal tissues were detected by immunohistochemical EliVision method. 2. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of SATB1 in 20 cases of supraglottic laryngeal carcinoma and 20 cases of normal tissues adjacent to glottic carcinoma. Expression of BRMS1mRNA and SATB1mRNA in supraglottic laryngeal carcinoma, 20 cases of glottic laryngeal carcinoma and the corresponding adjacent normal tissues. 3. To analyze the correlation between BRMS1 and SATB1 expression in laryngeal squamous cell carcinoma and the sex of the patients. Age, clinical TNM stage, degree of differentiation and lymph node metastasis. Results:. I. Immunohistochemical results. 1. The positive expression of BRMS1 protein in supraglottic laryngeal squamous cell carcinoma was significantly lower than that in adjacent normal tissues (P < 0.05). 2.The positive expression of BRMS1 protein in glottic laryngeal squamous cell carcinoma was significantly lower than that in adjacent normal tissues (P < 0.05). 3. The positive expression of BRMS1 protein in supraglottic laryngeal carcinoma was not significantly different from that of BRMS1 in glottic laryngeal carcinoma (P > 0.05). 4. The positive expression of SATB1 protein in supraglottic laryngeal squamous cell carcinoma was significantly higher than that in adjacent normal tissues (P < 0.05). 5.The positive expression of SATB1 protein in glottic laryngeal squamous cell carcinoma was higher than that in adjacent normal tissue (P < 0.05). 6. The positive expression of SATB1 protein in supraglottic laryngeal carcinoma was not significantly different from that in glottic laryngeal carcinoma (P > 0.05). 7. The expression of BRMS1 protein and SATB1 protein in supraglottic laryngeal carcinoma was related to TNM stage, tumor differentiation, lymph node metastasis, age and sex. 8. The expression of BRMS1 protein and SATB1 protein in glottic laryngeal carcinoma was related to TNM stage, tumor differentiation, age, sex, lymph node metastasis or not. 9. The expression of BRMS1 protein and SATB1 protein in supraglottic laryngeal carcinoma and glottic laryngeal carcinoma were negatively correlated. RT-PCR results. 1. In supraglottic type and glottic type group, the expression of BRMS1mRNA in laryngeal squamous cell carcinoma was lower than that in adjacent normal tissue (P < 0.05). 2. The expression of SATB1mRNA in the supraglottic type and glottic type was higher in laryngeal squamous cell carcinoma than in the adjacent normal tissue (P < 0.05). Conclusion:. The expression of 1.BRMS1 in supraglottic laryngeal carcinoma and glottic laryngeal carcinoma was lower than that in adjacent normal tissues. The expression of 2.SATB1 in supraglottic laryngeal carcinoma and glottic laryngeal carcinoma was higher than that in adjacent normal tissue. 3. The expression of BRMS1 and SATB1 in supraglottic laryngeal carcinoma was related to TNM stage, tumor differentiation, lymph node metastasis, age and sex. 4. The expression of BRMS1 and SATB1 in glottic laryngeal carcinoma was related to TNM stage, tumor differentiation, age, sex and lymph node metastasis. The expression of 5.BRMS1 and SATB1 was negatively correlated, and the combined detection of BRMS1 and SATB1 might be an important index to judge the lymph node metastasis of laryngeal squamous cell carcinoma (LSCC).
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.65

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