本文選題：應(yīng)激　切入點(diǎn)：應(yīng)激反應(yīng)　出處：《吉林大學(xué)》2011年碩士論文

【摘要】：目的研究應(yīng)激反應(yīng)時大鼠血清中腎上腺素濃度的變化,并通過觀察大鼠視網(wǎng)膜色素上皮細(xì)胞在應(yīng)激狀態(tài)下超微結(jié)構(gòu)的改變,探討應(yīng)激反應(yīng)與視網(wǎng)膜色素上皮細(xì)胞改變的相關(guān)性,為進(jìn)一步研究視網(wǎng)膜疾病的發(fā)生發(fā)展奠定理論基礎(chǔ)。 方法將24只健康雄性Wistar大鼠分成4組建立應(yīng)激大鼠模型,分別予以束縛應(yīng)激,誘導(dǎo)應(yīng)激反應(yīng)發(fā)生。收集大鼠血清樣本后通過酶聯(lián)免疫吸附法(ELISA),監(jiān)測大鼠血清中腎上腺素濃度變化,整理數(shù)據(jù),使用SPSS13.0統(tǒng)計(jì)學(xué)軟件進(jìn)行數(shù)據(jù)處理,并采用光鏡、透射電鏡技術(shù)對大鼠視網(wǎng)膜組織超微結(jié)構(gòu)進(jìn)行觀察,從而得出結(jié)論。 結(jié)果 (1).對照組大鼠血清腎上腺素濃度在正常飼養(yǎng)階段并未發(fā)生明顯的變化,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 (2).適應(yīng)組大鼠從實(shí)驗(yàn)第一周開始應(yīng)激,與第一周初相比,血清腎上腺素濃度明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05),第三周初停止應(yīng)激,從第三周末血清腎上腺素濃度開始下降,但是較第一周初仍有升高。 (3).適應(yīng)+應(yīng)激組大鼠從實(shí)驗(yàn)第一周開始應(yīng)激,與第一周初相比,血清腎上腺素濃度明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05),并且隨著應(yīng)激時間的延長血清腎上腺素濃度逐漸升高。 (4).應(yīng)激組大鼠從實(shí)驗(yàn)第三周開始應(yīng)激,與第一周初相比,血清腎上腺素濃度明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05),并且隨著應(yīng)激時間的延長血清腎上腺素濃度逐漸升高。 (5).普通光學(xué)顯微鏡觀察：RPE細(xì)胞未見明顯異常。透射電子顯微鏡觀察(JEM-1200EX):RPE細(xì)胞核形狀多不規(guī)則,細(xì)胞內(nèi)多種細(xì)胞器發(fā)生病理性改變,線粒體空化腫大,可見色素顆粒,偶見內(nèi)質(zhì)網(wǎng)輕度擴(kuò)張,且隨著腎上腺素濃度增加而愈發(fā)嚴(yán)重。 結(jié)論 (1).持續(xù)應(yīng)激可能使大鼠體內(nèi)腎上腺素水平較應(yīng)激前明顯增高,并且隨著應(yīng)激時間的延長血清腎上腺素濃度逐漸升高。 (2).終止應(yīng)激后大鼠體內(nèi)腎上腺素水平下降但仍較應(yīng)激前增高。 (3).應(yīng)激狀態(tài)下,腎上腺素濃度增高誘導(dǎo)RPE細(xì)胞內(nèi)出現(xiàn)缺氧性改變,并隨著應(yīng)激時間的延長RPE細(xì)胞損害加重。
[Abstract]:Objective to study the changes of serum epinephrine concentration in rats during stress, and to investigate the relationship between stress response and retinal pigment epithelial cells by observing the ultrastructural changes of retinal pigment epithelial cells under stress. To lay a theoretical foundation for the further study of the occurrence and development of retinal diseases. Methods 24 healthy male Wistar rats were divided into 4 groups to establish stress model and were subjected to restraint stress respectively. After collecting rat serum samples, the changes of epinephrine concentration in serum of rats were monitored, the data were processed by SPSS13.0 software, and light microscope was used. The ultrastructure of rat retina was observed by transmission electron microscopy (TEM). Results. The serum epinephrine concentration in the control group did not change significantly in the normal feeding stage, but the difference was not statistically significant (P 0.05). The rats in the adaptation group began to stress from the first week of the experiment. Compared with the beginning of the first week, the concentration of serum epinephrine increased significantly, the difference was statistically significant (P 0.05). At the beginning of the third week, the stress was stopped, and the concentration of serum adrenaline began to decrease at the end of the third week. But it's still up from the beginning of the first week. Compared with the first week, the concentration of serum epinephrine in the adaptation stress group was significantly higher than that in the first week, the difference was statistically significant (P 0.05), and the concentration of serum epinephrine gradually increased with the prolongation of the stress time. The rats in the stress group began to stress from the third week of the experiment. Compared with the first week, the concentration of serum epinephrine increased significantly, the difference was statistically significant (P 0.05), and the concentration of serum epinephrine gradually increased with the prolongation of the stress time. Under ordinary optical microscope, there was no obvious abnormality in the RPE cells. Transmission electron microscopy (TEM) showed that the nuclei of JEM-1200EX1: RPE were irregular in shape, many organelles in the cells had pathological changes, mitochondria were cavitated and swollen, and pigment granules could be seen. Occasionally, endoplasmic reticulum dilated slightly, and became more serious with the increase of epinephrine concentration. Conclusion. The level of epinephrine in rats was obviously higher than that before stress, and the concentration of serum epinephrine increased gradually with the prolongation of stress time. After termination of stress, the level of adrenaline in rats decreased, but it was still higher than that before stress. Under stress, the increased concentration of epinephrine induced hypoxic changes in RPE cells, and the damage of RPE cells increased with the prolongation of stress time.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R774.1

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