本文選題：間歇低氧　切入點(diǎn)：心肌重塑　出處：《山西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：摘要： 目的： 通過(guò)構(gòu)建間歇低氧大鼠模型，檢測(cè)模型大鼠心肌肥厚指數(shù)；觀察大鼠心肌細(xì)胞形態(tài)學(xué)變化；測(cè)定心肌組織中p38MAPK、CARP蛋白表達(dá)量的變化，探討間歇低氧對(duì)心肌重塑的影響。 方法： 選取健康雄性SD大鼠24只，隨機(jī)分3組：間歇低氧4周組（CIH4）、間歇低氧8周組（CIH8），常氧組（NC）每組8只；將間歇低氧組大鼠放入低氧艙中，交替通入氮?dú)馀c氧氣，分別于4周、8周時(shí)處死CIH4組和CIH8組大鼠，用分析天平測(cè)定各組體重、左心室重量及左室肥厚指數(shù)；對(duì)大鼠心肌組織行HE染色，觀察各組心肌組織形態(tài)學(xué)變化；用免疫組織化學(xué)法測(cè)定各組大鼠左心室p38MAPK、CARP蛋白表達(dá)，用灰度值表示其表達(dá)量。 結(jié)果 1.與NC組大鼠相比，CIH4、CIH8組大鼠心肌肥厚指數(shù)增加，CIH8組更為明顯（P＜0.05，，有統(tǒng)計(jì)學(xué)意義）； 2.對(duì)大鼠左心室心肌進(jìn)行形態(tài)學(xué)研究，顯示CIH4、CIH8組心肌細(xì)胞排列紊亂、有部分心肌纖維化，CIH8組更為明顯。 3.CIH4、CIH8組p38MAPK蛋白表達(dá)較NC組增加，CIH8組較CIH4組更為明顯（P＜0.05，有統(tǒng)計(jì)學(xué)意義）； 4.CIH4、CIH8組CARP蛋白表達(dá)較NC組增加，CIH8組較CIH4組更為明顯（P＜0.05，有統(tǒng)計(jì)學(xué)意義）； 5. p38MAPK與CARP兩個(gè)指標(biāo)采用Person相關(guān)分析，發(fā)現(xiàn)兩者具有明顯相關(guān)性。 結(jié)論： 1.間歇低氧可能為引起大鼠心肌重塑的誘因之一； 2. p38MAPK與CARP的表達(dá)在CIH4、CIH8組大鼠心肌組織中上調(diào)，且隨著低氧暴露時(shí)間的延長(zhǎng)表達(dá)量增加明顯； 3. p38MAPK與CARP的表達(dá)具有相關(guān)性，提示兩者的共同作用可能是間歇低氧大鼠發(fā)生心肌重塑的機(jī)制之一。
[Abstract]:Abstract:. Objective:. The myocardial hypertrophy index was measured, the morphological changes of myocardial cells were observed, and the expression of p38MAPKnCARP protein in myocardial tissue was measured to investigate the effect of intermittent hypoxia on myocardial remodeling. Methods:. Twenty-four healthy male SD rats were randomly divided into three groups: intermittent hypoxia group (4 weeks), intermittent hypoxia group (8 weeks), intermittent hypoxia group (8 rats), normoxic group (n = 8), and intermittent hypoxia group rats were placed in hypoxic chamber and alternately injected with nitrogen and oxygen. The rats of CIH4 group and CIH8 group were killed at 4 weeks and 8 weeks, the weight, left ventricular weight and left ventricular hypertrophy index of each group were measured by analytical balance, the myocardial tissue of rats was stained with HE, and the morphologic changes of myocardium were observed in each group. Immunohistochemical method was used to detect the expression of p38 MAPK- CARP protein in left ventricle of rats in each group, and the expression of p38 MAPK- CARP protein was expressed by gray value. Results. 1.Compared with the NC group, the myocardial hypertrophy index of the CIH4 + CIH8 group was significantly higher than that of the CIH8 group (P < 0.05). 2. The morphologic study of left ventricular myocardium in rats showed that the arrangement of myocardial cells in CIH4 + CIH8 group was disordered, and some myocardial fibrosis was more obvious in CIH8 group. 3. The expression of p38 MAPK protein in CIH4 + CIH8 group was significantly higher than that in CIH4 group (P < 0.05). 4. The expression of CARP protein in CIH4 + CIH8 group was significantly higher than that in CIH4 group (P < 0.05). 5. The correlation between p38 MAPK and CARP was analyzed by Person, and it was found that there was a significant correlation between p38 MAPK and CARP. Conclusion:. 1. Intermittent hypoxia may be one of the inducements of myocardial remodeling in rats. 2. The expression of p38 MAPK and CARP was up-regulated in the myocardial tissue of CIH4 + CIH8 group, and the expression of p38 MAPK and CARP increased significantly with the prolongation of hypoxia exposure time. 3. The expression of p38 MAPK is correlated with the expression of CARP, which suggests that the co-action of p38 MAPK and p38 MAPK may be one of the mechanisms of myocardial remodeling in intermittent hypoxic rats.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R766

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 李曉東,高楓;阻塞性睡眠呼吸暫停綜合征與心腦血管疾病[J];北京醫(yī)學(xué);2005年11期

2 李向東;覃數(shù);;p38 MAPK在心肌肥厚中的作用機(jī)制[J];重慶醫(yī)學(xué);2007年14期

3 王國(guó)付,鄭海農(nóng),欽光躍;阻塞性睡眠呼吸暫停低通氣綜合征與心腦血管疾病的關(guān)系[J];心腦血管病防治;2005年05期

4 張挪富,鐘南山;阻塞性睡眠呼吸暫停低通氣綜合征與腦血管疾病的關(guān)系[J];中華結(jié)核和呼吸雜志;2003年09期

5 楊麗霞,魏玲,王先梅,郭傳明,齊峰,任麗;p38MAPK信號(hào)通路的變化在心力衰竭病人心肌重塑中的意義[J];中國(guó)急救醫(yī)學(xué);2002年05期

本文編號(hào)：1640469