本文選題：三七總皂苷　切入點：結(jié)膜下出血　出處：《昆明醫(yī)科大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：[目的] 建立兔眼結(jié)膜下出血的模型,設(shè)立衡量結(jié)膜下出血的療效指標及定量指標,探討三七總皂苷(PNS)的藥理作用,證實三七總皂苷滴眼劑局部治療兔眼結(jié)膜下出血的可行性和有效性。 [方法] 選取健康成年新西蘭大白兔20只(40眼),分成用藥組和空白對照兩大組,其中用藥組按藥物濃度不同又分為高濃度組、中濃度組、低濃度組,每組5只(10眼),采用自體靜脈血結(jié)膜下定量注射的方法,建立結(jié)膜下出血模型。建模后對用藥組給予三七總皂苷滴眼劑治療,其中低濃度組用0.5%三七總皂苷滴眼劑,中濃度組為1%,高濃度組為2%,待結(jié)膜下出血治愈后停藥,空白對照組不給予任何治療。建模后每天裂隙燈顯微鏡下觀察各樣本結(jié)膜下出血量的變化,并記錄治愈時間,設(shè)定療效指標：5d內(nèi)治愈為顯效,6-7d治愈為有效,7d后仍未治愈為無效,按療效指標統(tǒng)計各組病例數(shù),然后采用SPSS17.0軟件進行K-W檢驗及U檢驗；同時于建模后1d、3d、5d、7d、9d通過裂隙燈顯微拍攝各樣本16倍結(jié)膜像,采用Image-Pro Plus (IPP)專業(yè)圖形處理軟件對其分析處理,測算結(jié)膜下出血區(qū)域的面積(Area)、平均光密度(MOD)、累計光密度(IOD)作為定量指標,然后采用SPSS17.0軟件進行重復(fù)測量的方差分析。通過以上兩種方法衡量三七總皂苷局部治療兔眼結(jié)膜下出血的效果。 [結(jié)果] 1.以治愈時間作為衡量標準：用藥各組(低濃度組、中濃度組、高濃度組)的平均治愈時間小于空白對照組,其總有效率也明顯高于空白對照組,且與用藥濃度呈正比；各組的療效差異有統(tǒng)計學(xué)意義(P0.05)。進一步兩兩比較結(jié)果顯示：療效在低濃度與空白對照組間、中濃度組與空白對照組間、高濃度組與空白對照組間、低濃度組與高濃度組間,差異有統(tǒng)計學(xué)意義(P0.008)；療效在低濃度組與中濃度組間、中濃度組與高濃度組間,差異無統(tǒng)計學(xué)意義(P0.008)。 2.以出血面積(Area)作為定量指標：各組Area值均隨著時間延長呈降低趨勢,其中高濃度組降低的趨勢最為顯著；Area在各組不同時相的差異有顯著性(P0.05)。組間兩兩比較結(jié)果顯示：Area在用藥各組與空白對照組比較,差異有統(tǒng)計學(xué)意義(P0.05),但是用藥組各組(高濃度組、中濃度組、低濃度組)之間比較,差異無統(tǒng)計學(xué)意義(P0.05)。 3.以平均光密度(MOD)作為定量指標：各組MOD均隨著時間延長呈降低趨勢,其中高濃度組降低的趨勢最為顯著；MOD在各組不同時相的差異有顯著性(P0.05)。組間兩兩比較結(jié)果顯示：MOD在低濃度組與空白對照組間、中濃度組與高濃度組間,差異無統(tǒng)計學(xué)意義(P0.05),其余組間兩兩比較,差異均有統(tǒng)計學(xué)差異(P0.05) 4.以累積光密度(IOD)作為定量指標：各組IOD均隨著時間延長呈降低趨勢,其中高濃度組降低趨勢最為顯著；IOD在各組不同時相的差異有顯著性(P0.05)。組間兩兩比較結(jié)果顯示：IOD在低濃度組與高濃度組間、中濃度組與空白對照組間、高濃度組與空白對照組間,差異有統(tǒng)計學(xué)意義(P0.05)；其余組間兩兩比較,差異無統(tǒng)計學(xué)意義(P0.05)。 [結(jié)論] 1.運用結(jié)膜下定量注射自體靜脈血的方法,可成功建立結(jié)膜下出血的動物模型,且可統(tǒng)一模型的出血量,使實驗處理因素標準化。 2.采用PNS滴眼劑治療兔眼結(jié)膜下出血,觀察比較各組治愈時間,并定量比較各組結(jié)膜下出血的Area、MOD、IOD,證實PNS滴眼劑可加快結(jié)膜下出血的吸收,使出血面積顯著縮小,出血區(qū)域顏色變淺,縮短病程,且其療效與用藥濃度呈正比。 3.運用IPP分析處理裂隙燈顯微鏡下采集的結(jié)膜像,測算出血區(qū)域Area、 MOD、IOD,作為衡量結(jié)膜下出血的定量指標,更為客觀有效。其中IOD既考慮了出血面積的大小,又反映了出血區(qū)域血色的深淺,較之Area和MOD更全面的測定結(jié)膜下血量,IOD是衡量結(jié)膜下出血量的最優(yōu)指標。
[Abstract]:[Objective]
A rabbit model of subconjunctival hemorrhage was established. A set of indicators and quantitative indicators for measuring subconjunctival hemorrhage were set up to investigate the pharmacological effects of 37 total saponins (PNS). It was proved that 37 total saponins eye drops were effective and effective in the treatment of rabbit subconjunctival hemorrhage.
[method]
Healthy adult 20 New Zealand white rabbits (40 eyes), divided into treatment group and control group two, the treatment group according to different drug concentrations and divided into high concentration group, middle concentration group, low concentration group, 5 rats in each group (10 eyes), using the method of quantitative injection of autologous venous blood under the conjunctiva. The establishment of subconjunctival hemorrhage model. After modeling of total saponins from 37 eyedrops in treatment of treatment group, the low concentration group of total saponins from 37 eye drops by 0.5%, the concentration of group was 1%, the high concentration group was 2%, the cure for subconjunctival hemorrhage after drug withdrawal, blank control group was not given any treatment. The amount of bleeding of the sample under the conjunctiva observation day after modeling the slit lamp microscope, and record the cure time, set the efficacy index: 5D cure is effective, cure 6-7d effectively, 7d has not been cured as invalid, according to the efficacy index statistics the number of each group, and then using SPSS17.0 software K-W Test and U test; at the same time to model after 1D, 3D, 5D, 7d, 9D by slit lamp microscopic photography of each sample is 16 times as the conjunctiva (IPP), Image-Pro Plus professional graphics software for the analysis, regional estimates of subconjunctival hemorrhage area (Area), the average optical density (MOD), total the optical density (IOD) as a quantitative index, and then repeated measure ANOVA using SPSS17.0 software. Through the above two methods to measure the effect of total saponins of 37 local treatment of rabbit subconjunctival hemorrhage.
[results]
1. time to heal as a measure of the standard: the treatment group (low concentration group, middle concentration group, high concentration group) the average cure time is less than the blank control group, the total efficiency was significantly higher than the control group, and the drug concentration is proportional to the difference was statistically significant; the effect of (P0.05). A further 22 comparison the results showed that the curative effect in low concentration and the blank control group, middle concentration group and blank control group, high concentration group and blank control group, low concentration group and high concentration group, the difference was statistically significant (P0.008); the curative effect in the low concentration group and middle concentration group, middle concentration group and the high concentration group, the difference was not statistically significant (P0.008).
2. to the area of bleeding (Area) as a quantitative index: the Area value was decreased with time, which reduce the high concentration group was the most significant trend; Area groups in different phases with significant difference (P0.05) between the 22 groups was compared. The results showed that Area in each treatment group and blank control group comparison, the difference was statistically significant (P0.05), but the treatment group (high concentration group, middle concentration group, low concentration group) comparison, no statistically significant difference (P0.05).
In 3. the average optical density (MOD) as a quantitative index: MOD in both groups decreased with time, which reduce the high concentration group was the most significant trend; MOD groups in different phases with significant difference (P0.05) between the 22 groups was compared. The results showed that MOD in low concentration group and blank control group between the middle concentration group and high concentration group, the difference was not statistically significant (P0.05), the comparison between the 22 groups, there were statistically significant difference (P0.05)
4. with the integrated optical density (IOD) as a quantitative index: IOD in both groups decreased with time, the high concentration group decreased most significantly; IOD groups in different phases with significant difference (P0.05) between the 22 groups was compared. The results showed that IOD in low concentration group and high concentration group and in the concentration group and blank control group, high concentration group and blank control group, the difference was statistically significant (P0.05); the comparison between the 22 groups, the difference was not statistically significant (P0.05).
[Conclusion]
1., using the method of quantitative injection of autologous venous blood under conjunctiva, we can successfully establish animal models of subconjunctival hemorrhage, and we can unify the amount of bleeding and standardize the experimental factors.
2. using PNS eyedrops in treatment of rabbit subconjunctival hemorrhage, observed the healing time, Area, hemorrhage and quantitative comparison of subconjunctival MOD, IOD PNS, confirmed that the eye drops can accelerate the absorption of subconjunctival hemorrhage, the hemorrhage area reduced significantly, the bleeding area pale, shorten the course, and the the efficacy and drug concentration.
3. by IPP analysis under the slit lamp microscope and collected conjunctival like, measurement of hemorrhagic area Area, MOD, IOD, as a measure of subconjunctival hemorrhage, which is more objective and effective. IOD can account for the bleeding area, but also reflects the depth of blood bleeding area, subconjunctival blood volume determination is more comprehensive the Area and MOD, IOD is the optimal index measure of subconjunctival bleeding.

【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R777.39

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本文編號：1638898