本文選題：形覺(jué)剝奪性近視　切入點(diǎn)：鞏膜　出處：《青島大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的：通過(guò)對(duì)形覺(jué)剝奪性近視豚鼠模型脈絡(luò)膜上腔注射外源性TIMP-2基因,觀察近視早期后極部鞏膜MMP-2的表達(dá),探討外源性TIMP-2基因?qū)髽O部鞏膜MMP-2表達(dá)的影響。 方法：采用單眼形覺(jué)遮蓋法對(duì)3周齡三色豚鼠進(jìn)行形覺(jué)剝奪,2w后通過(guò)檢影驗(yàn)光和測(cè)量眼軸長(zhǎng)度證實(shí)FDM模型是否建立成功。從模型建立成功的豚鼠中隨機(jī)選取60只,隨機(jī)分成4組,分別為T(mén)IMP-2組(脈絡(luò)膜上腔注射外源性TIMP-2基因)、空質(zhì)粒組(脈絡(luò)膜上腔注射空質(zhì)粒)、生理鹽水組(脈絡(luò)膜上腔注射生理鹽水)、對(duì)照組,每組15只。TIMP-2組、空質(zhì)粒組、生理鹽水組對(duì)右眼完成注射操作后繼續(xù)遮蓋,其中TIMP-2組左眼為自身對(duì)照組；對(duì)照組右眼持續(xù)遮蓋不作任何處理。分別于注射后2d、7d、14d采集后極部鞏膜標(biāo)本,用RT-PCR及Western Blot法檢測(cè)MMP-2 mRNA及蛋白的表達(dá),統(tǒng)計(jì)學(xué)分析各組之間及不同時(shí)間表達(dá)的差異。 結(jié)果：(1)注射后TIMP-2組MMP-2 mRNA及蛋白的表達(dá)出現(xiàn)先降低后增高的變化,注射后2d與7d MMP-2 mRNA及蛋白表達(dá)變化有顯著性差異(P0.05),7d與14d表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。注射后2d、7d、14d,TIMP-2組、自身對(duì)照組、對(duì)照組之間比較,MMP-2 mRNA及蛋白的表達(dá)變化均有統(tǒng)計(jì)學(xué)意義(均P0.05)；空質(zhì)粒組、生理鹽水組、對(duì)照組之間比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(均P0.05)。(2)注射后TIMP-2組TIMP-2 mRNA表達(dá)出現(xiàn)先增加后減少的變化。注射后2d與7d表達(dá)變化有統(tǒng)計(jì)學(xué)意義(P0.05),7d與14d差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。注射后2d、7d、14d, TIMP-2組、自身對(duì)照組、對(duì)照組之間比較,TIMP-2 mRNA表達(dá)變化均有統(tǒng)計(jì)學(xué)意義(均P0.05)；空質(zhì)粒組、生理鹽水組、對(duì)照組之間比較,表達(dá)變化無(wú)統(tǒng)計(jì)學(xué)意義(均P0.05)。 結(jié)論：外源性TIMP-2基因轉(zhuǎn)染能明顯抑制形覺(jué)剝奪性近視眼后極部鞏膜MMP-2 mRNA及蛋白的表達(dá),這種改變?cè)缙诰鸵呀?jīng)開(kāi)始發(fā)生,隨著轉(zhuǎn)染后時(shí)間的延長(zhǎng)出現(xiàn)先降低后增高的變化。
[Abstract]:Aim: to investigate the effect of exogenous TIMP-2 gene on the expression of MMP-2 in posterior sclera of early myopia by injecting exogenous TIMP-2 gene into the superior choroidal cavity of form-deprived myopia guinea pig model. Methods: the successful establishment of FDM model was confirmed by optometry and eye axis length measurement after 3 weeks old tricolor guinea pigs were deprived of form sensation by monocular shading method. 60 guinea pigs were randomly selected from the successful model. They were randomly divided into four groups: TIMP-2 group (intrachoroidal injection of exogenous TIMP-2 gene), empty plasmid group (suprachoroidal cavity injection of empty plasmids), saline group (intrachoroidal injection of normal saline), control group (15 rats in each group, TIMP-2 group, empty plasmid group). Normal saline group continued to cover the right eye after the injection, in which the left eye of the TIMP-2 group was the self-control group, while the right eye of the control group was continuously covered without any treatment. The sclera specimens were collected at 2 days, 7 days and 14 days after injection, respectively. The expression of MMP-2 mRNA and protein was detected by RT-PCR and Western Blot. Results the expression of MMP-2 mRNA and protein in TIMP-2 group decreased first and then increased after injection. There was no significant difference in the expression of MMP-2 mRNA and protein between 2 and 7 days after injection. There was no significant difference in the expression of MMP-2 mRNA and protein between 7 and 14 days after injection. There were significant differences in the expression of MMP-2 mRNA and protein between the control group and the control group (all P 0.05), the comparison between the blank plasmid group, the normal saline group and the control group. The expression of TIMP-2 mRNA in TIMP-2 group increased first and then decreased after injection. There was no significant difference in the expression of TIMP-2 mRNA between 2 days and 7 days after injection. There was no significant difference in the expression of TIMP-2 mRNA between 2 days and 7 days after injection. There was no significant difference in the expression of TIMP-2 mRNA between 2 days after injection and 14 days after injection (P 0. 05%). The expression of TIMP-2 mRNA in TIMP-2 group was 14 days after injection, while in TIMP-2 group and self control group, there was no significant difference between 2 days and 14 days after injection. The changes of TIMP-2 mRNA expression in the control group were statistically significant (P 0.05), but there was no significant difference between the blank plasmid group, normal saline group and control group (all P 0.05). Conclusion: exogenous TIMP-2 gene transfection can significantly inhibit the expression of MMP-2 mRNA and protein in the posterior sclera of form-deprived myopia, and this change has begun to occur at the early stage. With the extension of transfection time, the expression of MMP-2 mRNA and protein in the posterior sclera is decreased first and then increased.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R778.11

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 尚慶麗,馬景學(xué),高健,吳洪濤,張博學(xué),邢敏;脂質(zhì)體介導(dǎo)endostatin基因轉(zhuǎn)染抑制脈絡(luò)膜新生血管的實(shí)驗(yàn)研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2004年18期

2 王劍鋒,劉雙珍,吳文燦,譚星平,江海波;外源性視黃酸對(duì)雞后鞏膜基質(zhì)MMP-2表達(dá)作用的動(dòng)態(tài)觀察[J];國(guó)際眼科雜志;2004年05期

3 毛俊峰,劉雙珍,魏欣;bFGF對(duì)雞形覺(jué)剝奪性近視眼的抑制作用[J];國(guó)際眼科雜志;2005年04期

4 潘佳鴻;基質(zhì)金屬蛋白酶及其在眼科的研究進(jìn)展[J];國(guó)外醫(yī)學(xué).眼科學(xué)分冊(cè);2003年06期

5 方麗君;朱益華;徐國(guó)興;;睫狀神經(jīng)營(yíng)養(yǎng)因子轉(zhuǎn)基因治療視神經(jīng)損傷的研究進(jìn)展[J];國(guó)際眼科縱覽;2006年02期

6 陳曉鋒,顧振綸,梁中琴,周文軒,郭次儀;基質(zhì)金屬蛋白酶與腫瘤侵襲和轉(zhuǎn)移研究進(jìn)展[J];中國(guó)藥理學(xué)通報(bào);2001年03期

7 高巖;崔冬梅;吳開(kāi)力;黃強(qiáng);曾駿文;;形覺(jué)剝奪性近視豚鼠鞏膜形態(tài)學(xué)觀察[J];眼科研究;2006年03期

8 吳文燦;劉雙珍;顏文韜;王劍峰;譚星平;;外源性基質(zhì)金屬蛋白酶抑制劑GM6001對(duì)FDM形成的抑制作用[J];眼科研究;2006年04期

9 瞿佳;堅(jiān)持防治近視眼研究的正確方向[J];中華眼科雜志;2003年06期

10 胡文政,褚仁遠(yuǎn);近視眼鞏膜的變化及其發(fā)生機(jī)制[J];眼視光學(xué)雜志;2001年01期

，

本文編號(hào)：1627215