本文選題：血管生成素-1　切入點(diǎn)：血管內(nèi)皮生長(zhǎng)因子　出處：《石河子大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討血管生成素-1(angiopoietin-1, Ang-1)和血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor, VEGF)在早期糖尿病大鼠視網(wǎng)膜中的表達(dá)及其意義。 方法：制備類似人類I型糖尿病大鼠動(dòng)物模型,取40只SD雄性大鼠分糖尿病組28只及止常對(duì)照組12只,糖尿病組28只SD雄性大鼠腹腔注射STZ60mg.Kg1,建模成功后分別在1周、2周、3周、4周取糖尿病組各7只及正常對(duì)照組3只摘取大鼠眼球,應(yīng)用免疫組化法檢測(cè)Ang-1、VEGF在視網(wǎng)膜中的表達(dá)。 結(jié)果：Ang-1在1~4周止常對(duì)照組大鼠視網(wǎng)膜切片上未見陽(yáng)性表達(dá),在1~3周糖尿病大鼠視網(wǎng)膜的切片上內(nèi)界膜、內(nèi)核層、神經(jīng)節(jié)細(xì)胞層及散在血管旁細(xì)胞免疫反應(yīng)陽(yáng)性呈棕黃色,定位于雙極細(xì)胞、Muller細(xì)胞及血管旁周細(xì)胞胞漿,陽(yáng)性表達(dá)并成上升趨勢(shì),4周時(shí)糖尿病大鼠視網(wǎng)膜中血管間隙增寬,視網(wǎng)膜厚度變薄,但血管形態(tài)無(wú)明顯改變,Amg-1表達(dá)陽(yáng)性率降低,已成陰性表達(dá)。Ang-1在糖尿病大鼠視網(wǎng)膜中表達(dá)1周陽(yáng)性率約為70.0%,2周陽(yáng)性率約為80.0%,3周陽(yáng)性率約為83.3%,4周陽(yáng)性率約為22.2%,已為陰性表達(dá)。糖尿病組4周之間Ang-1陽(yáng)性率進(jìn)行X2檢驗(yàn),X2=21.67,P=0.010(P0.05)有統(tǒng)計(jì)學(xué)意義,正常組與糖尿病1~3周間Ang-1的表達(dá)陽(yáng)性率統(tǒng)計(jì)學(xué)分析有顯著性差異(P0.01),而與糖尿病4周組無(wú)差異(P＞0.05)。VEGF在1~4周正常大鼠對(duì)照組切片上視網(wǎng)膜各層均可見陰性染色反應(yīng),而在1~4周糖尿病模型組切片上均可見位于視網(wǎng)膜內(nèi)核層的雙極細(xì)胞、色素上皮細(xì)胞層以及散在的血.管內(nèi)皮細(xì)胞、Muller細(xì)胞的染色反應(yīng),1~3周出現(xiàn)陽(yáng)性表達(dá)上升趨勢(shì),4周表達(dá)有所下降,但仍在范圍之內(nèi)。VEGF在糖尿病大鼠視網(wǎng)膜中表達(dá)1周陽(yáng)性率約為75.0%,2周陽(yáng)性率約為85.9%,3周陽(yáng)性率約為90.0%,4周陽(yáng)性率約為73.2%。在部分切片的內(nèi)界膜亦可見淺棕色弱陽(yáng)性反應(yīng)；4周時(shí)可見毛細(xì)血管間隙增寬。糖尿病組4周之間VEGF陽(yáng)性率進(jìn)行X2檢驗(yàn),X2=14.45,P0.05。正常對(duì)照組與糖尿病各組問(wèn)VEGF的表達(dá)陽(yáng)性率進(jìn)行統(tǒng)計(jì)學(xué)分析有差異P0.05。 結(jié)論:1、糖尿病大鼠4周時(shí)視網(wǎng)膜已出現(xiàn)明顯改變：視網(wǎng)膜厚度變薄,血管壁間隙增寬,未見血管旁細(xì)胞。2、Ang-1、VEGF在早期糖尿病大鼠視網(wǎng)膜中1~3周出現(xiàn)高表達(dá),4周時(shí)表達(dá)下降,且二者出現(xiàn)分離現(xiàn)象,推測(cè)隨著病程的延長(zhǎng),血管通透性隨著增加,Ang-1表達(dá)會(huì)越來(lái)越少,而VEGF表達(dá)會(huì)越來(lái)越多。3、Ang-1作為抗?jié)B透因子,VEGF為滲透因子,在血管完整性中起著重要的作用,二者失去平衡后,即出現(xiàn)血管滲透,從而產(chǎn)生刺激血管新生的因子釋放。由此明確了Ang-1在早期糖尿病視網(wǎng)膜中的表達(dá),可作為以后糖尿病視網(wǎng)膜病變?cè)缙诟深A(yù)治療指南措施之一。
[Abstract]:Aim: to investigate the expression and significance of angiopoietin-1 (Ang-1) and vascular endothelial growth factor (VEGF) in retina of early diabetic rats. Methods: animal models similar to human type I diabetes were established. Forty SD male rats were divided into diabetic group (n = 28) and control group (n = 12). Twenty-eight male SD rats in the diabetic group were injected intraperitoneally with STZ60 mg 路Kg1.After the model was successfully established, 7 rats in the diabetic group and 3 rats in the control group were enucleated at 1 week, 2 weeks, 3 weeks and 4 weeks, respectively. The expression of Ang-1VEGF in the retina was detected by immunohistochemical method. Results there was no positive expression of VAng-1 on the retinal sections of the control group from 1 to 4 weeks, but the immunoreactivity of the upper inner boundary membrane, the nuclear layer, the ganglion cell layer and the paravascular cells in the retina slices of the diabetic rats at the 1st and 3rd week were brown and yellow. The positive expression of amg-1 was observed in the cytoplasm of the Muller cells and perivascular cells located in the bipolar cells. The positive expression of Amg-1 in the retina of diabetic rats increased after 4 weeks, but the positive rate of Amg-1 expression was decreased, but the retinal thickness became thinner, but there was no obvious change in the morphology of the blood vessels, but the positive rate of Amg-1 expression was decreased. The positive rate of negative expression of Ang-1 in the retina of diabetic rats was 70.0and the positive rate was about 80.03 weeks after 2 weeks. The positive rate of positive expression of Ang-1 in the retina of diabetic rats was about 83.3 and 22.2.The positive rate of Ang-1 in the diabetic group was determined by X2 between 4 weeks and 4 weeks. There was statistical significance in the test of X2 (21.67) and P0. 010 (P0. 05). There was significant difference in the positive rate of Ang-1 expression between the normal group and the diabetic group within 3 weeks, but there was no significant difference between the normal group and the 4 week diabetic group (P > 0.05). Negative staining was observed in all layers of the retina of the normal control group at the 1st and 4th week. However, bipolar cells located in the nuclear layer of the retina were found on the slices of the 1-week diabetic model group. The staining reaction of pigment epithelial cells and scattered blood. The positive expression of Muller cells increased in the 1st week and decreased in the 4th week. However, the positive rate of VEGF expression in the retina of diabetic rats was 75.0 in 1 week and 85.9 in 2 weeks. The positive rate was 90.0 in 4 weeks and 73.2 percent in 4 weeks. The light brown weak positive reaction was also found in the inner limiting membrane of some sections. The positive rate of VEGF was detected by X2 test during 4 weeks in diabetic group. The positive rate of VEGF expression in normal control group and diabetic group was analyzed statistically (P0.05). ConclusionRetina of diabetic rats showed obvious changes at 4 weeks: the thickness of retina became thinner, the space of vascular wall widened, and the expression of VEGF in the retina of early diabetic rats decreased at 4 weeks after the high expression of VEGF was found in the retina of early diabetic rats at 1 ~ 3 weeks. It is assumed that with the prolongation of the course of disease, the expression of Ang-1 will decrease with the increase of vascular permeability, while the expression of VEGF will be more and more as an osmotic factor, which plays an important role in vascular integrity. When the two are out of balance, blood vessel osmosis occurs, which leads to the release of factors that stimulate angiogenesis. This makes clear the expression of Ang-1 in the retina of early diabetes mellitus. It can be used as one of the early intervention guidelines for diabetic retinopathy.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R774.1

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