本文選題：豚鼠　切入點：動物模型　出處：《復旦大學》2011年碩士論文　論文類型：學位論文

【摘要】：第一部分豚鼠內(nèi)淋巴積水模型的建立 目的：探討豚鼠內(nèi)淋巴積水模型的制作方法,為后續(xù)實驗奠定基礎。方法：健康豚鼠30只,隨機分為對照組、實驗組1、實驗組2,各10只,實驗組1、實驗組2行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動物模型,對照組右側(cè)施行假手術,于術后四周、術后八周分別處死實驗組1、實驗組2,術后八周處死對照組,取雙側(cè)聽泡進行切片并染色。結(jié)果：對照組及左側(cè)耳蝸均未出現(xiàn)膜迷路積水,實驗組1、實驗組2出現(xiàn)程度不同膜迷路積水,前庭膜向前庭階膨出。實驗組2較實驗組1顯示前庭膜膨出更嚴重。結(jié)論：內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞術可以導致豚鼠內(nèi)淋巴積水,有效建立了膜迷路積水的動物模型。 第二部分豚鼠眼震電圖的記錄 目的：探討豚鼠的眼震電圖的記錄方法,從而了解內(nèi)淋巴積水對豚鼠外周前庭頻率感受功能的損傷特點。方法：健康豚鼠30只,隨機分為正常對照組、前庭破壞組、實驗組,各10只,實驗組行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動物模型,前庭破壞組行前庭破壞術。用冰水試驗和旋轉(zhuǎn)試驗誘發(fā)并記錄正常對照組、前庭破壞組的眼震電圖,于術后四周用冰水試驗和旋轉(zhuǎn)試驗誘發(fā)并記錄實驗組的眼震電圖。眼震電圖記錄眼震持續(xù)時間和頻率。結(jié)果：冰水試驗正常對照組可引出明顯眼震,并持續(xù)較長時間。實驗組術后四周冰水刺激未引出眼震,前庭破壞組冰水試驗無眼震。在旋轉(zhuǎn)試驗中,前庭破壞組未引出明顯眼震。正常對照組和實驗組術后四周時左、右眼震慢相速度無統(tǒng)計學差異(P0.05)。在甩頭試驗中,前庭破壞組無明顯眼動,正常對照組和實驗組術后四周時左、右眼動速度無統(tǒng)計學差異(P0.05)。結(jié)論：內(nèi)淋巴積水主要影響前庭器官的低頻旋轉(zhuǎn)感受功能,高頻、高速旋轉(zhuǎn)無明顯影響。 第三部分內(nèi)淋巴積水豚鼠各時期眼震變化的記錄 目的：探討內(nèi)淋巴豚鼠不同時期眼震的變化情況,從而了解內(nèi)淋巴積水不同時期對前庭功能的損傷特點。方法：健康豚鼠40只,隨機分為對照組、術后兩周組、術后四周組、術后八周組4組,術后組均行右側(cè)內(nèi)淋巴囊切除及部分內(nèi)淋巴囊阻塞以建立內(nèi)淋巴積水動物模型,于相應時間段用冰水試驗和旋轉(zhuǎn)試驗誘發(fā)并記錄眼震電圖。并同期處死豚鼠,取水平半規(guī)管、上半規(guī)管、后半規(guī)管、橢圓 囊、球囊標本,進行掃描、透射電鏡觀察。結(jié)果：冰水試驗時術后兩周組有4只豚鼠(40%)引出眼震,但是眼震較微弱,持續(xù)時間較對照組短、眼震速度較慢。其余術后各組均未引出明顯眼震。旋轉(zhuǎn)試驗時對照組、術后兩周組、術后四周組、術后八周組左、右眼震慢相速度無明顯差異(P0.05)。甩頭試驗時,對照組、術后兩周組、術后四周組、術后八周組左、右眼動速度無明顯差異(P0.05)。電鏡中可見術后兩周時毛細胞無明顯改變,四周時部分區(qū)域毛細胞排列不規(guī)整,纖毛變短、倒伏,Ⅱ型毛細胞腫脹、空泡,Ⅰ型毛細胞形態(tài)較完整。八周時部分區(qū)域出現(xiàn)毛細胞的缺失,Ⅱ型毛細胞出現(xiàn)變形、皺縮,Ⅰ型毛細胞形態(tài)無明顯變形。結(jié)論：內(nèi)淋巴積水主要影響前庭器官的低頻旋轉(zhuǎn)感受功能,對前庭毛細胞的損傷多以Ⅱ型毛細胞為主。Ⅱ型毛細胞的損傷和缺失可能是低頻旋轉(zhuǎn)感受功能損害和缺失的主要細胞學基礎。
[Abstract]:The first part of the model of endolymphatic hydrops in guinea pigs
Objective: To explore the method of making guinea pig endolymphatic hydrops model, lay the foundation for the subsequent experiments. Methods: 30 guinea pigs were randomly divided into control group, experimental group 1, experimental group 2, 10 rats in each experimental group 1, experimental group 2 underwent right hepatectomy and partial endolymphatic sac endolymphatic sac occlusion to establish endolymphatic hydrops animal model, the control group underwent sham operation on the right side, four weeks after surgery, eight weeks after operation respectively. The experimental group 1, experimental group 2, control group were sacrificed eight weeks after operation, the bilateral auditory vesicle were sliced and stained. Results: the control group and the left cochlea were not hydrolabyrinth, experiment group 1, experimental group 2 different degrees of hydrolabyrinth, vestibular membrane forward bulging. The court order 2 in experimental group than in the experimental group 1 showed more severe vestibular meningocele. Conclusion: endolymphatic sac excision and endolymphatic sac occlusion can lead to guinea pig endolymphatic hydrops, established a membranous labyrinth An animal model of water accumulation.
The record of second parts of guinea pig nystagmus
Objective: To explore the method of recording electronystagmogram of guinea pigs, so as to understand the injury characteristics of endolymphatic hydrops in guinea pig vestibular function of frequency. Methods: 30 guinea pigs were randomly divided into normal control group, vestibular damage group, experimental group, 10 rats in each experimental group underwent right hepatectomy and partial in endolymphatic sac in order to establish the obstruction of endolymphatic sac endolymphatic hydrops animal model of vestibular damage group underwent vestibular destruction. With ice water test and rotation test induced and recorded in normal control group, damage group vestibular nystagmus, at four weeks after surgery with ice water test and rotation test induced and electronystagmography recording experimental group electronystagmogram duration were recorded. And the frequency of ice water test. Results: the normal control group can lead to obvious nystagmus, and lasting a long time. After the experimental group around the ice water stimulation did not elicit vestibular nystagmus, nystagmus. No damage group ice test In the rotarod test, vestibular damage were not elicited obvious nystagmus. Normal control group and experimental group four weeks after surgery when left, there was no significant difference in right nystagmus slow phase velocity (P0.05). In the head test, no obvious damage to vestibular eye group, normal control group and experimental group after four weeks left, no significant difference right eye velocity (P0.05). Conclusion: endolymphatic hydrops mainly affects the vestibular organ low-frequency rotation sensation function, high frequency, high rotating speed has no obvious effect.
Changes of nystagmus in third parts of endolymphatic hydrops in guinea pigs
Objective: To investigate the changes of guinea pig endolymphatic nystagmus, so as to understand the endolymphatic hydrops damage characteristics of vestibular function in different period. Methods: 40 guinea pigs were randomly divided into control group, two weeks after surgery group, postoperative 4 weeks group, eight weeks after the operation of 4 groups, postoperative groups the right part of the endolymphatic sac excision and endolymphatic sac occlusion to establish endolymphatic hydrops animal model, in the corresponding time period with ice water test and rotation test induced and recording electronystagmogram and over the same period. The guinea pigs were sacrificed, the horizontal semicircular canal, anterior semicircular canal, posterior semicircular canal, oval
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本文編號：1606464