本文選題：Netrin-1　切入點(diǎn)：糖尿病視網(wǎng)膜病變　出處：《中南大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的檢測(cè)鏈脲佐菌素(Streptozotocin, STZ)誘導(dǎo)的糖尿病大鼠模型視網(wǎng)膜中Netrin-1mRNA的表達(dá)變化,探討Netrin-1在糖尿病視網(wǎng)膜病變(Diabetic retinopathy, DR)中可能存在的發(fā)病機(jī)理。 方法將20只成年雄性Sprague-Dawley (SD)大鼠隨機(jī)分為正常對(duì)照組(Normal control, NC)與糖尿病組(Diabetic model, DM),每組各10只,用提前預(yù)冷的PH4.5的檸檬酸緩沖液溶解STZ, DM組按照60mg/kg體重劑量一次性腹腔注射新鮮配制的1%STZ,對(duì)照組腹腔注射0.5mL檸檬酸緩沖液。注藥48h后測(cè)空腹尾靜脈血糖≥16.67mmol/L者定義為糖尿病組大鼠,每個(gè)月測(cè)量糖尿病大鼠體重及血糖。糖尿病大鼠成模后3個(gè)月處死,采用Trizol法提取大鼠視網(wǎng)膜組織總RNA,應(yīng)用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)技術(shù)檢測(cè)NC組和DM組大鼠視網(wǎng)膜中Netrin-1mRNA的表達(dá)差異。 結(jié)果(1)STZ誘導(dǎo)的大鼠糖尿病模型的建立：STZ誘導(dǎo)糖尿病大鼠成模率為90%,1只大鼠注射STZ后血糖未升高而淘汰,剩余9只糖尿病組大鼠注射STZ后出現(xiàn)糖尿病多飲、多食、多尿、體重下降的典型“三多一少”糖尿病癥狀,隨時(shí)間延長(zhǎng)毛發(fā)逐漸失去光澤,行動(dòng)較遲緩,精神稍差。與正常對(duì)照組相比,糖尿病組大鼠的血糖值明顯升高,兩者之間有顯著性差異(p0.01)；體重增長(zhǎng)較正常對(duì)照組明顯下降,兩者之間有顯著性差異(p0.01)；3個(gè)月時(shí)糖尿病大鼠均發(fā)生了明顯的白內(nèi)障。(2)RT-PCR檢測(cè)結(jié)果均顯示,Netrin-1mRNA在正常對(duì)照組大鼠視網(wǎng)膜中弱表達(dá),在糖尿病組中表達(dá)較對(duì)照組明顯增強(qiáng),兩者比較有統(tǒng)計(jì)學(xué)意義(p0.05)。 結(jié)論STZ誘導(dǎo)糖尿病大鼠是一種簡(jiǎn)便易行的制作糖尿病視網(wǎng)膜病變的動(dòng)物模型；Netrin-1mRNA在糖尿病誘導(dǎo)3個(gè)月的大鼠視網(wǎng)膜中表達(dá)較正常對(duì)照組明顯增強(qiáng),提示Netrin-1參與了早期糖尿病視網(wǎng)膜病變的發(fā)生發(fā)展,可能與視網(wǎng)膜血管的通透性增加有關(guān)。
[Abstract]:Objective to detect the expression of Netrin-1mRNA in the retina of diabetic rats induced by streptozotocin (STZ) and to explore the possible pathogenesis of Netrin-1 in diabetic retinopathy. Methods Twenty adult male Sprague-Dawley rats were randomly divided into normal control group (NC) and diabetic model (DM) with 10 rats in each group. STZs were dissolved with citric acid buffer of precooled PH4.5 in advance. In DM group, fresh 1 STZ was injected intraperitoneally according to 60 mg / kg body weight, and 0.5 mL citric acid buffer was injected intraperitoneally in control group. 48 hours after injection, fasting caudal vein blood glucose 鈮，

本文編號(hào)：1566454