發(fā)布時(shí)間：2018-02-23 13:49

  本文關(guān)鍵詞： 早產(chǎn)兒視網(wǎng)膜病/病因?qū)W 抗原 CD147 疾病模型 動(dòng)物　出處：《濱州醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的建立氧誘導(dǎo)視網(wǎng)膜病變大鼠模型,觀察其視網(wǎng)膜各層組織中CD147、基質(zhì)金屬蛋白酶(MMP)-2和血管內(nèi)皮生長因子(VEGF)的表達(dá)情況。方法1、將68只新生Wistar大鼠隨機(jī)分為高氧組和對(duì)照組。每組各34只大鼠。高氧誘導(dǎo)實(shí)驗(yàn)組形成氧誘導(dǎo)視網(wǎng)膜病變模型；對(duì)照組不予任何處理。2、新生鼠7、14日齡時(shí),取出眼球后行視網(wǎng)膜鋪片,觀察視網(wǎng)膜血管形態(tài)學(xué)變化。3、新生鼠14日齡時(shí),取出眼球后行視網(wǎng)膜石蠟切片,計(jì)數(shù)突破視網(wǎng)膜內(nèi)界膜的內(nèi)皮細(xì)胞核數(shù)。4、新生鼠12、14、16日齡時(shí),取眼球行免疫組織化學(xué)染色法,檢測(cè)視網(wǎng)膜各層組織中CD147、MMP-2和VEGF的表達(dá)。5、運(yùn)用雙因素相關(guān)分析法,解析氧誘導(dǎo)視網(wǎng)膜病變模型中CD147表達(dá)與MMP-2、VEGF表達(dá)的關(guān)聯(lián)性。結(jié)果1、新生鼠7日齡時(shí),對(duì)照組大鼠視網(wǎng)膜血管自視盤呈放射狀散開,走形清晰,血管徑較粗；高氧組大鼠視網(wǎng)膜血管收縮、彎曲,周邊散在廣泛的無血管區(qū),或僅見為數(shù)不多的小面積的血管組織網(wǎng)。新生鼠14日齡時(shí),對(duì)照組大鼠視網(wǎng)膜血管形態(tài)發(fā)育較好,走形正常,網(wǎng)格規(guī)整；高氧組大鼠出現(xiàn)視網(wǎng)膜血管增生、紆曲、擴(kuò)張,并于灌注區(qū)與非灌注區(qū)交界處形成新生血管叢,部分發(fā)生滲出、出血。2、新生鼠14日齡時(shí),對(duì)照組、高氧組突破網(wǎng)膜內(nèi)界膜的內(nèi)皮細(xì)胞核數(shù)分別為(1.30±1.26)、(19.70±3.56)個(gè)；兩組比較,差異有統(tǒng)計(jì)學(xué)意義(t=21.813,P0.01)。3、免疫組織化學(xué)染色結(jié)果顯示,對(duì)照組大鼠視網(wǎng)膜各層均可見CD147、MMP-2及VEGF有少量表達(dá)。而高氧組大鼠視網(wǎng)膜各層均可見CD147、MMP-2、VEGF有顯著表達(dá)。在新生鼠12、14、16日齡時(shí),將兩組大鼠視網(wǎng)膜各層中CD147(t=5.612、 11.390、6.355)、MMP-2(t=4.122、8.047、4.422)、VEGF (t=4.955、12.176、5.110)表達(dá)相較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。4、采用相關(guān)性分析發(fā)現(xiàn),CD147的表達(dá)與MMP-2、VEGF的表達(dá)均具有正相關(guān)性(r=0.755、0.820,P0.01)。結(jié)論CD147、MMP-2、VEGF在氧誘導(dǎo)視網(wǎng)膜病變大鼠視網(wǎng)膜中有大量表達(dá)。
[Abstract]:Objective to establish a rat model of oxygen-induced retinopathy. To observe the expression of CD147, matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in various layers of retina. Methods 1. Sixty-eight newborn Wistar rats were randomly divided into hyperoxia group and control group, 34 rats in each group were induced by hyperoxia. Oxygen induced retinopathy model was formed in the experimental group. In the control group, no treatment was given. At the age of 714 days, retinal slices were taken out and retinal vascular morphology was observed. At the age of 14 days, paraffin sections of the retina were taken out from the eyes of the newborn rats. The number of endothelial nuclei that broke through the inner membrane of the retina was counted. At the age of 1416 days, the eyes of the newborn rats were taken for immunohistochemical staining to detect the expression of CD147 MMP 2 and VEGF in all layers of the retina, and the expression of MMP-2 and VEGF were detected by double factor correlation analysis. Results 1. At the age of 7 days, the retinal vascular self-vision disc of the control group was radially dispersed, the shape was clear, and the diameter of the blood vessel was relatively large. The retinal vessels in hyperoxia group were constricted, bent and scattered in a wide, non-vascular area, or only a few small vascular tissue networks were found. At 14 days of age, the retinal vessels in the control group developed well and the shape of retinal vessels was normal. In hyperoxia group, retinal vessels proliferated, tortuous, dilated, and formed neovascularization plexus at the junction of perfusion area and non-perfusion area. The number of endothelial nuclei in the hyperoxia group was 1.30 鹵1.26, 19.70 鹵3.56, respectively, and the difference was statistically significant between the two groups. The results of immunohistochemical staining showed that the number of endothelial nuclei in the hyperoxia group was 21.813 (P 0.01), and that in the hyperoxia group was higher than that in the control group (P < 0.05). The expression of CD147MMP 2 and VEGF were observed in all layers of the retina of rats in the control group, while in the hyperoxia group, the expression of CD147MMP 2 was found in all layers of the retina of the hyperoxia group. At the age of 1416 days, the expression of CD147MMP 2 in the different layers of the retina of the two groups was 5.612, 11.3906.355MMP 2t was 4.1228.047.4.422VEGF-VEGFtt 4.955n12.1765.110), and the expression of CD147MMP 2 in the different layers of the retina of the control group was higher than that of the control group. The correlation analysis showed that there was a positive correlation between the expression of CD147 and the expression of MMP-2VEGF. Conclusion the expression of CD147 and MMP-2VEGF is highly expressed in oxygen-induced retinopathy rats.
【學(xué)位授予單位】：濱州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R774.1

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相關(guān)期刊論文 前1條

1 曹宏力;早產(chǎn)兒視網(wǎng)膜病變的研究進(jìn)展[J];中國優(yōu)生與遺傳雜志;2005年08期

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本文編號(hào)：1526804