本文關(guān)鍵詞： 準(zhǔn)分子激光角膜切削術(shù) 酒精 大鼠 角膜上皮 緊密連接　出處：《安徽醫(yī)科大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討屈光手術(shù)中酒精法去上皮對大鼠角膜上皮緊密連接的影響。 方法:60只SD大鼠隨機(jī)分為酒精組和機(jī)械組,各30只。在各組內(nèi)再將大鼠隨機(jī)六組(A、B、C、D、E、F),每組5只,每只大鼠隨機(jī)選擇一只眼作為手術(shù)眼,另一只眼作為對照眼。分別用來觀察24 h大鼠角膜上皮缺損直徑、檢測24、48、72、96、120 h角膜上皮claudin-1、ZO-1蛋白的表達(dá)情況。心臟注藥法處死動物,取眼球,用OCT冰凍包埋,制作冰凍切片,HE染色,免疫熒光法檢測角膜上皮緊密連接蛋白表達(dá)光密度,進(jìn)行統(tǒng)計學(xué)比較。 結(jié)果: 1.去上皮時間:酒精組和機(jī)械組去上皮時間的差異有統(tǒng)計學(xué)意義(P0.05)。 2.角膜上皮中央缺損直徑:24 h手術(shù)顯微鏡下觀察60只大鼠的手術(shù)眼,中央?yún)^(qū)角膜上皮均有缺損。裂隙燈照相分析酒精組和機(jī)械組A組大鼠術(shù)眼角膜上皮缺損直徑的大小,酒精組和機(jī)械組角膜上皮缺損直徑差異無統(tǒng)計學(xué)意義。手術(shù)顯微鏡下觀察48 h實(shí)驗(yàn)大鼠手術(shù)眼角膜上皮光滑,均未見缺損。 3.組織病理學(xué)改變:HE染色結(jié)果顯示兩組48 h移行的角膜上皮完全覆蓋損傷的部位,但僅2～3層上皮細(xì)胞,細(xì)胞形態(tài)異常,變大、扁平延長。96 h角膜上皮基本恢復(fù)正常形態(tài)。 4. ZO-1蛋白表達(dá)差異:ZO-1主要在角膜上皮表層細(xì)胞表達(dá),24、48 h酒精組和機(jī)械組角膜上皮ZO-1蛋白的表達(dá)差異有統(tǒng)計學(xué)意義(P0.05)。72、96、120 h ZO-1蛋白的表達(dá)在酒精組和機(jī)械組差異無統(tǒng)計學(xué)意義。 5. claudin-1蛋白表達(dá)差異:claudin-1主要在角膜上皮翼狀細(xì)胞和基底細(xì)胞層表達(dá),24、48 h酒精組和機(jī)械組角膜上皮claudin-1蛋白的表達(dá)差異有統(tǒng)計學(xué)意義(P0.05)。72、96、120 h claudin-1蛋白的表達(dá)在酒精組和機(jī)械組差異無統(tǒng)計學(xué)意義。 結(jié)論:屈光手術(shù)手術(shù)中酒精法去上皮較機(jī)械法去上皮速度快,二者角膜上皮再生時間未見明顯區(qū)別。但酒精法去上皮較機(jī)械法影響角膜上皮緊密連接的修復(fù),說明20%酒精去上皮影響角膜上皮的修復(fù)。
[Abstract]:Objective: To investigate the effect of alcohol removal on the close connection of corneal epithelium in rats during refractive surgery.
Methods: 60 SD rats were randomly divided into alcohol group and mechanical group, 30 rats each. The rats in groups randomly six groups (A, B, C, D, E, F), 5 rats in each group, each rat were randomly selected as one eye surgery eye, the other eye as the control eyes were used to observe the corneal epithelial defect. 24 h rats to detect 24,48,72,96120 h in diameter, corneal epithelial claudin-1, expression of ZO-1 protein. The heart injection were animal, eyeballs, frozen OCT embedding, the production of frozen sections, HE staining, immunofluorescence detection of corneal epithelial tight junction protein expression in the light density. The results were statistically analyzed.
Result:
1. the time to go to the epithelium: there was a significant difference in the time between the alcohol group and the mechanical group (P0.05).
The diameter of 2. central corneal epithelial defect: Observation of 60 rats 24 h eye surgery under surgical microscope, central corneal epithelium defect. Slit lamp diameter and mechanical analysis in alcohol group group A group rat corneal epithelial defect size, alcohol group and mechanical corneal epithelial defect diameter was no significant difference in operation. Under the microscope 48 h rat corneal epithelial surgery is smooth, no defect.
3. histopathological changes: HE staining showed that two h 48.96 migrated corneal epithelium completely covered the site of injury, but only 2~3 layers of epithelial cells, abnormal cell morphology, larger, flat, prolonged.96 h corneal epithelium was basically restored to normal form.
4., the difference of ZO-1 protein expression: ZO-1 was mainly expressed in the epithelial cells of corneal epithelium. The expression of ZO-1 protein in corneal epithelium of 24,48 h alcohol group and mechanical group was statistically significant (P0.05). The expression of.72,96120 h ZO-1 protein in alcohol group and mechanical group was not statistically significant.
5. claudin-1 expression: claudin-1 mainly expressed in the epithelium of cells and basal cell layer, there was significant difference in the expression of 24,48 h in alcohol group and mechanical corneal epithelial claudin-1 protein (P0.05) expression of.72,96120 h claudin-1 protein had no significant difference in the alcohol group and mechanical group.
Conclusion: alcohol refractive surgery operation. Compared with the mechanical method to epithelial epithelial fast, two corneal epithelial regeneration time had no significant difference. But the alcohol method to epithelium mechanical effects on corneal epithelial tight junction repair, 20% alcohol to repair the corneal epithelium epithelium.

【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R779.63

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