本文關(guān)鍵詞： Th17細胞 佛波脂 塵螨變應(yīng)原 變應(yīng)性鼻炎 佛波脂 塵螨變應(yīng)原 記憶性T細胞 輔助T細胞　出處：《華中科技大學》2010年碩士論文　論文類型：學位論文

【摘要】：目的:探討常年變應(yīng)性鼻炎患者外周血中Th17細胞的功能狀況。方法:選取塵螨過敏性常年性變應(yīng)性鼻炎患者(AR)及健康對照者(HC)各10人。從外周血分離單個核細胞(PBMCs),分為離子霉素組(空白組),塵螨組, PMA組:離子霉素組加入離子霉素1ug/ml和莫能霉素2umol/l;塵螨組加入離子霉素1ug/ml和塵螨變應(yīng)原nDer p1 1ug/ml及莫能霉素2umol /l;PMA組加入PMA 20ng/ml,離子霉素1ug/ml和莫能霉素2umol/l,在37°C,5%CO2細胞培養(yǎng)箱培養(yǎng)6h。用流式細胞技術(shù)檢測Th17細胞占CD4+T細胞百分比,分析常年變應(yīng)性鼻炎患者外周血中Th17細胞的功能。結(jié)果:HC空白組、塵螨組和PMA組Th17細胞百分比均低于對應(yīng)的AR(p 0.05);經(jīng)PMA刺激后,HC和ARTh17細胞百分比較空白組均增高(p 0.05), AR增幅更大;經(jīng)nDerp1刺激后,HCTh17百分比較空白組無明顯改變,而ARTh17百分比較空白組顯著降低(p 0.05)。結(jié)論:提示Th17細胞可能參與變應(yīng)性鼻炎的發(fā)病機制。PMA刺激可以引起能分泌IL-17的CD4+T數(shù)量增加。 目的:探討常年變應(yīng)性鼻炎患者外周血中記憶性Th1和Th2細胞的功能狀況。方法:選取塵螨過敏性常年性變應(yīng)性鼻炎患者(AR)及健康對照者(HC)各10人。從外周血分離單個核細胞,分為離子霉素組(空白組),塵螨組,PMA組:離子霉素組加入離子霉素1ug/ml和莫能霉素2umol/l;塵螨組加入離子霉素1ug/ml和塵螨變應(yīng)原nDer p1 1ug/ml及莫能霉素2umol /l;PMA組加入PMA 20ng/ml,離子霉素1ug/ml和莫能霉素2umol/l,在37°C,5%CO2細胞培養(yǎng)箱培養(yǎng)6h。培養(yǎng)6小時后,收集PBMCs,進行胞膜和胞內(nèi)染色,用流式細胞技術(shù)檢測記憶性Th1/Th2細胞占CD4+CD45RO+T(Tm)細胞百分比,分析常年變應(yīng)性鼻炎患者外周血中記憶性Th1/Th2細胞的功能。結(jié)果:HC空白組外周血中記憶性的Th2百分比高于AR空白組,經(jīng)塵螨刺激后,HC記憶性的Th2百分比無明顯改變,而AR增加,且效應(yīng)性的Th2百分比增加更明顯(p 0.05)。HC外周血中記憶性的Th1百分比高于AR,經(jīng)塵螨刺激后,HC和AR均下降,但是HC下降更明顯,雖然沒有統(tǒng)計學差異(P0.05)。經(jīng)PMA刺激后,HC和AR外周血中記憶性的Th2百分比均顯著下降(p 0.05);HC和AR外周血中記憶性的Th1百分比均顯著上升(p 0.05)。結(jié)論:AR存在對塵螨變應(yīng)原的特異性免疫記憶性Th2細胞。PMA可以使CD4+T細胞分泌IFN -γ增加,分泌IL-4減少。
[Abstract]:Objective: to investigate the function of Th17 cells in peripheral blood of patients with perennial allergic rhinitis. Methods: 10 patients with allergic perennial allergic rhinitis and 10 healthy controls were selected. Mononuclear cells were isolated from peripheral blood. PBMCs were divided into ionomycin group (blank group, dust mite group, PMA group: ionomycin 1ugrml and monomycin 2umolrl added in ionomycin group; dust mites group added ionomycin 1ugrml and dust mite allergen nDer p11ugrml and monenomycin 2umoll; PMA group added PMA 20ng.ml. The ratio of Th17 cells to CD4 T cells was detected by flow cytometry, which was cultured in 37 擄C ~ (5) CO _ 2 cell incubator at 37 擄C ~ (5) for 6 h. The function of Th17 cells in peripheral blood of patients with perennial allergic rhinitis was analyzed. Results the percentage of Th17 cells in the control group, dust mite group and PMA group was lower than that in the corresponding AR(p 0.05% group, and the percentage of Th17 and ARTh17 cells after PMA stimulation was higher than that in the control group. After nDerp1 stimulation, HCTh17% did not change significantly compared with the control group, while the ARTh17 percentage decreased significantly compared with the control group (P 0.05). Conclusion: the results suggest that Th17 cells may participate in the pathogenesis of allergic rhinitis. PMA stimulation can induce the increase of CD4 T secreting IL-17. Objective: to investigate the function of memory Th1 and Th2 cells in peripheral blood of patients with perennial allergic rhinitis. Methods: ten patients with allergic perennial allergic rhinitis and 10 healthy controls were selected to isolate mononuclear cells from peripheral blood. It was divided into ionomycin group (blank group, dust mite group, PMA group: ionomycin 1ugrml and monomycin 2umoll added in ionomycin group; dust mites group added ionomycin 1ugrml and dust mite allergen nDer p11ugrml and monenomycin 2umoll; PMA group added PMA 20ng / ml, ionomycin / ml; 1 g / ml and 2 umoll / l of monomycin, cultured in 37 擄C 5 CO 2 cell incubator for 6 hours, 6 hours later, PBMC cells were collected for cell membrane and intracellular staining, and the percentage of memory Th1/Th2 cells in CD4 CD45RO TmTm cells was detected by flow cytometry. The function of memory Th1/Th2 cells in peripheral blood of patients with perennial allergic rhinitis was analyzed. Results the percentage of Th2 in peripheral blood of the control group was higher than that of the group of AR blank, but the percentage of Th2 of memory of HC was not changed after stimulation by dust mites, but AR increased. The percentage of effectual Th2 in peripheral blood was significantly higher than that of Th1 in peripheral blood, but HC and AR decreased significantly after dust mites stimulation, but the decrease of HC was more obvious. Although there was no statistical difference (P 0.05), the percentage of memory Th2 in peripheral blood of HC and AR decreased significantly after stimulation by PMA. The percentage of Th1 of memory in both HC and AR increased significantly. Conclusion\% AR has a positive effect on dust mite allergen. Specific immune memory Th2 cells. PMA could increase the secretion of IFN-緯 by CD4 T cells. Secretion of IL-4 decreased.
【學位授予單位】：華中科技大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R765.21

【共引文獻】

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2 鄒華;全反式維甲酸對博萊霉素誘導的小鼠肺纖維化的干預的實驗研究[D];昆明醫(yī)學院;2011年

3 盧宏松;Flt3L分子佐劑對LCMV肽疫苗特異性免疫應(yīng)答的影響[D];暨南大學;2009年

4 唐春燕;VIP對LPS應(yīng)激肺內(nèi)IL-17及其受體表達的影響與機制[D];中南大學;2010年

5 李樹民;Th17細胞及其相關(guān)因子CCR6、RORγt在大鼠肺纖維化模型中表達的意義[D];佳木斯大學;2013年

6 張明;IL-17A拮抗劑對成纖維細胞增殖和窖蛋白-1表達的影響[D];瀘州醫(yī)學院;2013年

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本文編號：1516075