本文關(guān)鍵詞： 羅格列酮 凋亡 鼻咽癌 放射增敏 細(xì)胞周期　出處：《中南大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討羅格列酮對人鼻咽癌CNE-2細(xì)胞增殖抑制和放射增敏作用及可能機(jī)制。 方法：利用MTT法測定羅格列酮對鼻咽癌CNE-2細(xì)胞的半數(shù)抑制濃度(IC50)；應(yīng)用克隆形成法和單靶多擊模型擬合放射劑量-細(xì)胞存活曲線,觀察羅格列酮對鼻咽癌CNE-2細(xì)胞株的放射增敏作用；吖啶橙染色與AnnexinV/PI雙染流式細(xì)胞技術(shù)檢測細(xì)胞凋亡；PI單染流式細(xì)胞技術(shù)觀察羅格列酮對CNE-2細(xì)胞周期分布的影響。 結(jié)果：羅格列酮對在體外培養(yǎng)的CNE-2細(xì)胞有不同程度的生長抑制作用,且在一定范圍內(nèi),隨處理時間的延長和給藥濃度的增加,其對CNE-2細(xì)胞的抑制率逐漸升高。羅格列酮在24h、48h、72h對CNE-2細(xì)胞的IC50分別為114.07μmol/L、97.46μmol/L、73.35μmol/L�？寺⌒纬蓪嶒烇@示羅格列酮聯(lián)合放射線作用于CNE-2細(xì)胞表現(xiàn)為劑量曲線左移,SER增加、Do值減低、Dq值變小細(xì)胞的放射敏感性增強(qiáng),羅格列酮對鼻咽癌CNE-2細(xì)胞株有明顯的放射增敏效應(yīng)；流式細(xì)胞術(shù)檢測顯示羅格列酮在較高濃度可誘導(dǎo)人鼻咽癌CNE-2細(xì)胞凋亡在低毒濃度下未能誘導(dǎo)CNE-2細(xì)胞凋亡,但能使其阻滯在G2/M期從而導(dǎo)致細(xì)胞周期再分布。 結(jié)論：羅格列酮對CNE-2細(xì)胞有增殖抑制和放射增敏作用,放射增敏的機(jī)理可能與羅格列酮使CNE-2細(xì)胞阻滯在G2/M期并使S期細(xì)胞比例減少導(dǎo)致細(xì)胞周期再分布有關(guān)。
[Abstract]:Aim: to investigate the effect of rosiglitazone on proliferation inhibition and radiosensitization of human nasopharyngeal carcinoma (NPC) CNE-2 cells and its possible mechanism. Methods: the half-inhibitory concentration of rosiglitazone on nasopharyngeal carcinoma (NPC) CNE-2 cells was determined by MTT assay, and the radiation dose-cell survival curve was fitted by clone formation method and single-target multi-shot model. The radiosensitizing effect of rosiglitazone on nasopharyngeal carcinoma (NPC) CNE-2 cell line was observed, and the effect of rosiglitazone on the distribution of CNE-2 cell cycle was observed by acridine orange staining and AnnexinV/PI double staining flow cytometry. Results: rosiglitazone could inhibit the growth of CNE-2 cells cultured in vitro, and in a certain range, with the prolongation of treatment time and the increase of drug concentration, rosiglitazone could inhibit the growth of CNE-2 cells in vitro. The inhibitory rate of rosiglitazone on CNE-2 cells gradually increased. The IC50 of rosiglitazone on CNE-2 cells was 114.07 渭 mol / L, 97.46 渭 mol / L, 73.35 渭 mol / L at 24 h and 48 h / 72 h, respectively. Clonogenic assay showed that rosiglitazone combined with radiation induced by rosiglitazone on CNE-2 cells showed a dose curve of left shift of SER, increase of do value and decrease of DQ. The radiosensitivity of small cells was increased. Rosiglitazone had a significant radiosensitization effect on nasopharyngeal carcinoma CNE-2 cell line, flow cytometry showed that rosiglitazone could induce apoptosis of human nasopharyngeal carcinoma CNE-2 cell line at higher concentration and failed to induce CNE-2 cell apoptosis at low concentration. But it can block it in G 2 / M phase and lead to cell cycle redistribution. Conclusion: rosiglitazone can inhibit the proliferation and radiosensitization of CNE-2 cells. The mechanism of radiosensitization may be related to the cell cycle redistribution caused by rosiglitazone blocking CNE-2 cells in G 2 / M phase and reducing the proportion of S phase cells.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R965;R739.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王雯s，

本文編號：1502423