本文關(guān)鍵詞： 大鼠 噪聲 聽(tīng)性腦干反應(yīng) 聽(tīng)皮層 谷氨酸脫羧酶　出處：《聽(tīng)力學(xué)及言語(yǔ)疾病雜志》2014年03期 　論文類(lèi)型：期刊論文

【摘要】：目的研究噪聲暴露對(duì)大鼠聽(tīng)性腦干反應(yīng)(auditory brainstem response,ABR)及聽(tīng)皮層谷氨酸脫羧酶67(glutamic acid decarboxylase-67,GAD67)表達(dá)的影響。方法將21只健康雌性SD大鼠隨機(jī)分為噪聲暴露后0、7、14天組,每組7只,每組再隨機(jī)分為對(duì)照組(3只)和暴露組(4只);將暴露組大鼠暴露于頻率4kHz以上、100dB SPL白噪聲2小時(shí)建立噪聲性聽(tīng)覺(jué)損傷的動(dòng)物模型,對(duì)照組大鼠不作任何處理;分別于噪聲暴露前及暴露后0天(0.5~2小時(shí))、第7天、第14天時(shí)檢測(cè)各組大鼠的ABR反應(yīng)閾;取噪聲暴露后0天(2~4小時(shí))、第7天、第14天各組大鼠的聽(tīng)皮層切片,用免疫組織化學(xué)的方法標(biāo)記各組聽(tīng)皮層中GAD67陽(yáng)性神經(jīng)元數(shù)量。結(jié)果①在噪聲暴露后0.5~2小時(shí)暴露組大鼠的ABR反應(yīng)閾明顯高于對(duì)照組(P0.05),14天時(shí)基本恢復(fù)正常;②在噪聲暴露后2~4小時(shí)暴露組大鼠聽(tīng)皮層GAD67陽(yáng)性神經(jīng)元數(shù)量(78.76±16.11個(gè)/mm2)明顯多于對(duì)照組(38.43±9.27個(gè)/mm2)(P0.05),第7天和第14天時(shí)暴露組GAD67陽(yáng)性神經(jīng)元數(shù)量(32.22±8.61個(gè)/mm2和29.55±11.61個(gè)/mm2)明顯低于對(duì)照組(43.58±12.87個(gè)/mm2和43.14±12.44個(gè)/mm2)(均P0.05)。結(jié)論噪聲暴露使大鼠ABR反應(yīng)閾發(fā)生了暫時(shí)性閾移,聽(tīng)皮層GAD67陽(yáng)性神經(jīng)元數(shù)量在噪聲暴露后先增多后減少,可能與噪聲性聾發(fā)病機(jī)制有關(guān)。
[Abstract]:Objective to study the effects of noise exposure on the expression of auditory brainstem responseABR and glutamate decarboxylase-67 in auditory cortex of rats. Methods Twenty-one healthy female SD rats were randomly divided into two groups, 7 rats in each group. Each group was randomly divided into control group (n = 3) and exposure group (n = 4). The rats in the exposure group were exposed to 100 dB SPL white noise at frequencies above 4kHz for 2 hours to establish the animal model of noise-induced hearing impairment, and the rats in the control group were not treated with any treatment. Before and 0 days after noise exposure, the ABR response thresholds were measured on the 7th day and the 14th day, respectively, and the auditory cortex sections of the rats in each group were taken 0 days after noise exposure for 2 hours, 7 days and 14 days, respectively. The number of GAD67 positive neurons in the auditory cortex of each group was labeled with immunohistochemical method. Results 1 the ABR response threshold of the rats in the exposure group was significantly higher than that in the control group at 0. 5 and 2 hours after noise exposure on the 14th day after noise exposure. 2 hours after exposure, the number of GAD67 positive neurons in the auditory cortex of rats in the exposure group (78.76 鹵16.11 / mm2) was significantly higher than that in the control group (38.43 鹵9.27 / m-2 / mm2), and the number of GAD67 positive neurons in the exposed group was 32.22 鹵8.61 / mm2 and 29.55 鹵11.61 / mm2 on the 7th and 14th days after exposure respectively, which was significantly lower than that in the control group (43.58 鹵12.87 / mm2 / mm2). And 43.14 鹵12.44 / mm2 (all P 0.05N). Conclusion noise exposure causes transient threshold shift of ABR reaction threshold in rats. The number of GAD67 positive neurons in auditory cortex increased first after noise exposure and then decreased, which may be related to the pathogenesis of noise-induced deafness.
【作者單位】： 安徽醫(yī)科大學(xué)附屬安徽省立醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國(guó)家自然科學(xué)基金(81200741) 安徽省自然基金(11040606M174)資助
【分類(lèi)號(hào)】：R764

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郭海，盧妙如，，李仲孝，周正謀，劉志發(fā);胃潰瘍大鼠強(qiáng)噪聲暴露后血清骨鈣素含量變化的探討[J];中國(guó)骨質(zhì)疏松雜志;1996年02期

2 王菱芝,趙一鳴,紀(jì)巖,潘敦銀,潘清英,王華鳳,羅乾元,賈樹(shù)成,楊飛,張?jiān)律n,汪兆武,蔡勤,胡月娥;化肥生產(chǎn)噪聲暴露與高血壓的劑量

本文編號(hào)：1502233