本文關(guān)鍵詞： 慢性間歇性低氧(CIH) 胰島β細(xì)胞 細(xì)胞增殖 哺乳動(dòng)物雷帕霉素靶蛋白　出處：《南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版)》2014年07期 　論文類(lèi)型：期刊論文

【摘要】：目的:探討慢性間歇性低氧(chronic intermittent hypoxia,CIH)條件下哺乳動(dòng)物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及相關(guān)通路激活對(duì)大鼠胰島β細(xì)胞增殖的影響及可能機(jī)制。方法:24只成年雄性SD大鼠按隨機(jī)數(shù)字法平均分為兩組。CIH組通過(guò)低氧艙造模,低氧條件(60 S/N2,60 S/空氣,交替充入,保證艙內(nèi)氧氣濃度在5%~21%之間波動(dòng),每天8:30~16:30,共35 d)。酶聯(lián)免疫法檢測(cè)兩組血糖、血清胰島素水平并計(jì)算胰島素抵抗指數(shù)(HOMA-IR)和β細(xì)胞胰島素分泌功能(HOMA-β)�？诜咸烟欠z測(cè)兩組大鼠糖耐量,免疫熒光法檢測(cè)胰島β細(xì)胞增殖情況,免疫印跡法檢測(cè)胰腺組織mTOR及通路相關(guān)蛋白指標(biāo)表達(dá)。結(jié)果:與對(duì)照組相比,CIH組大鼠空腹血糖、血清胰島素水平及胰島素抵抗指數(shù)升高(P0.01);葡萄糖耐量、胰島β細(xì)胞功能降低(P0.05);ki67染色陽(yáng)性的β細(xì)胞約增加了5倍;胰腺組織mTOR通路相關(guān)蛋白激活。結(jié)論:CIH條件下大鼠發(fā)生胰島素抵抗、葡萄糖耐量降低、胰島β細(xì)胞功能下降,而mTOR相關(guān)通路的激活可能在胰島β細(xì)胞增殖中發(fā)揮作用。
[Abstract]:Objective: to study the chronic intermittent hypoxia of chronic intermittent hypoxia. Target of rapamycin. Methods 24 adult male Sprague-Dawley rats were randomly divided into two groups. The hypoxic condition is 60 S / N 2 and 60 S / air, which is filled alternately to ensure that the oxygen concentration in the cabin fluctuates between 5% and 21%, 8: 30% 16: 30% a day. The blood glucose was detected by enzyme-linked immunosorbent assay (Elisa) for 35 days. Serum insulin level and insulin resistance index (HOMA-IRI) and insulin secretion function of 尾 cells were calculated. Glucose tolerance was measured by oral glucose method. Immunofluorescence assay was used to detect the proliferation of islet 尾 cells, and Western blot was used to detect the expression of mTOR and pathway related protein in pancreatic tissue. Results: compared with the control group, the fasting blood glucose of rats in the control group was determined. Serum insulin level and insulin resistance index increased P0.01; Glucose tolerance, 尾 -cell function of islet decreased P0.05; The number of 尾 cells positive for ki67 staining increased by 5 times. Conclusion the insulin resistance, glucose tolerance and 尾 -cell function of pancreatic islets were decreased in rats with mTOR pathway activation. The activation of mTOR related pathway may play a role in the proliferation of islet 尾 cells.
【作者單位】： 南京醫(yī)科大學(xué)第一附屬醫(yī)院耳鼻咽喉科;
【基金】：南京醫(yī)科大學(xué)第一附屬醫(yī)院創(chuàng)新團(tuán)隊(duì)基金(IRT-16) 教育部留學(xué)回國(guó)人員科研啟動(dòng)基金(20101174)
【分類(lèi)號(hào)】：R766
【正文快照】： 阻塞性睡眠呼吸暫停綜合征(obstructive sleepapnea syndrome,OSAS)在正常成人中發(fā)病率為2%~4%[1],以睡眠過(guò)程中反復(fù)發(fā)生上氣道阻塞為特點(diǎn),導(dǎo)致呼吸暫停和睡眠片段化[2],從而引發(fā)一系列并發(fā)癥,如糖尿病、高血壓等[3-4]。臨床調(diào)查研究顯示在[Acta Univ Med Nanjing,2014,34(07)

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