本文關(guān)鍵詞：白血病抑制因子（LIF）促進(jìn)兔眼LASIK術(shù)后角膜神經(jīng)修復(fù)的實(shí)驗(yàn)研究　出處：《重慶醫(yī)科大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 角膜神經(jīng) 神經(jīng)再生 準(zhǔn)分子激光原位角膜磨鑲術(shù) 白血病抑制因子

【摘要】：目的:探討白血病抑制因子(Leukemia inhibitory factor,LIF)對兔眼準(zhǔn)分子激光原位角膜磨鑲術(shù)(laser in situ keratomileusis,LASIK)后角膜神經(jīng)修復(fù)的促進(jìn)作用,并觀察評估白血病抑制因子局部使用的安全性。 方法:40只健康新西蘭大白兔,其中5只兔作為空白組,不作任何處理,用于觀察正常兔眼的角膜神經(jīng)形態(tài);其余35只兔(70只眼)均接受近視性LASIK手術(shù),術(shù)后左眼給予自制的1.0μg/mlLIF滴眼液,4次/天,作為治療組;右眼給予平衡鹽溶液(balanced salt solution,BSS),4次/天,作為對照組。分別于術(shù)后第1天、第1周、第2周、第1個(gè)月、第3個(gè)月及第6個(gè)月進(jìn)行干眼參數(shù)檢查,并各取5只兔處死后摘取雙側(cè)角膜行氯化金染色,光鏡下觀察角膜神經(jīng)修復(fù)情況,并進(jìn)行兩組比較。余下5只兔于術(shù)后第6個(gè)月處死后摘下雙側(cè)角膜,每只角膜平均分成兩半,一半用于制作角膜病理切片,光鏡下觀察LIF滴眼液使用6個(gè)月后對角膜各層細(xì)胞結(jié)構(gòu)的影響,另一半行透射電鏡觀察LIF滴眼液使用6個(gè)月后對角膜各層超微結(jié)構(gòu)的影響。 結(jié)果:1.LASIK術(shù)后除蒂部以外,角膜瓣切削區(qū)上皮下及淺基質(zhì)層神經(jīng)均消失,術(shù)后第7天角膜瓣邊緣可見少量再生神經(jīng)纖維,第6個(gè)月時(shí)上皮下、淺基質(zhì)層神經(jīng)數(shù)量和形態(tài)接近正常,周邊部角膜神經(jīng)網(wǎng)密度與術(shù)前無明顯差異,而中央密度仍低于術(shù)前。 2. LASIK術(shù)后各時(shí)間點(diǎn)除第6個(gè)月時(shí),治療組神經(jīng)末梢數(shù)量均高于對照組,有統(tǒng)計(jì)學(xué)差異(P0.05)。 3.術(shù)后兩組干眼參數(shù)比較,第2周、1月、3月治療組均高于對照組,差異有統(tǒng)計(jì)學(xué)意義,其余各期比較無統(tǒng)計(jì)學(xué)差異。 4.LASIK術(shù)后局部使用LIF滴眼液治療的6個(gè)月內(nèi),并未發(fā)現(xiàn)角膜新生血管形成、上皮剝脫、晶體混濁等不良反應(yīng),光鏡和電鏡下觀察兔眼角膜各層結(jié)構(gòu)均無明顯變化。 結(jié)論:1. LASIK手術(shù)造成兔眼大部分角膜神經(jīng)的丟失,除蒂部以外,角膜瓣切削區(qū)上皮下及淺基質(zhì)層神經(jīng)均消失。 2.LIF能有效促進(jìn)LASIK術(shù)后兔眼角膜神經(jīng)的修復(fù),從而改善術(shù)后干眼現(xiàn)象,是一種安全有效的藥物。
[Abstract]:Objective: to investigate the leukemia inhibitory factor Leukemia inhibitory factor. The effect of lif on corneal nerve repair after laser in situ keratomileusis in situ keratomileusis in rabbits was studied. The safety of local use of leukemia suppressor was evaluated. Methods of 40 healthy New Zealand white rabbits, 5 rabbits were used as blank group, without any treatment, to observe the corneal nerve morphology of normal rabbits. The other 35 rabbits (70 eyes) were treated with myopic LASIK operation. The left eye was treated with self-made 1.0 渭 g / ml LIF eye drops 4 times a day as the treatment group. The right eye was treated with balanced salt solution four times a day as the control group, respectively on the first day, the first week and the second week after operation. The dry eye parameters were examined in the first month, the third month and the sixth month, and 5 rabbits were killed respectively. The corneas were harvested and stained with gold chloride. The nerve repair of the cornea was observed under light microscope. The remaining 5 rabbits were killed at the 6th month after operation. Each cornea was divided into two halves, half of which was used to make pathological sections of the cornea. The effects of LIF eye drops on the cell structure of each layer of cornea were observed under light microscope, and the effects of LIF eye drops on the ultrastructure of each layer of cornea were observed by transmission electron microscope (TEM). Results 1. After LASIK, the subcutaneous and superficial stromal layer nerves in the corneal flap disappeared except for the pedicle. A small amount of regenerated nerve fibers were observed at the edge of the corneal flap on the 7th day after operation, and at the 6th month. The number and morphology of the superficial stromal nerve were close to normal, the density of the peripheral corneal nerve network was not significantly different from that of the preoperative, but the central density was still lower than that of the preoperation. 2. At 6 months after LASIK, the number of nerve endings in the treatment group was higher than that in the control group (P 0.05). 3. The dry eye parameters of the two groups were higher than that of the control group in the 2nd week, January and March, the difference was statistically significant, but there was no statistical difference in the other stages. 4. Within 6 months after LASIK local use of LIF eye drops, no adverse reactions such as corneal neovascularization, epithelial exfoliation and lens opacity were found. There was no obvious change in corneal structure under light microscope and electron microscope. Conclusion 1. LASIK procedure caused the loss of most corneal nerves in rabbit eyes, and the subcutaneous and superficial stromal nerves in the corneal flap region disappeared except for the pedicle. 2. LIF can effectively promote the corneal nerve repair after LASIK and improve the dry eye phenomenon. It is a safe and effective drug.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R779.63

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