【摘要】：目的探究3種飲用水對(duì)高血壓小鼠血壓的影響及可能機(jī)制。方法測(cè)定3種飲用水的水質(zhì)情況:溶解性總固體(TDS)、耗氧量(OC)、p H、氧化還原電位(ORP)、電導(dǎo)率(EC)、溶解氫、鈣和鎂;采用N'-硝基-L-精氨酸甲酯鹽酸鹽(L-NAME)連續(xù)灌胃法(30天)建立ICR小鼠高血壓模型。然后將小鼠隨機(jī)分為4組:正常對(duì)照組、模型對(duì)照組、模型自來水組和模型過濾水組,每個(gè)月檢測(cè)小鼠血壓,3個(gè)月后測(cè)定小鼠血清一氧化氮(NO)、一氧化氮合酶(NOS)、總超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽過氧化物酶(GSH-Px)、內(nèi)皮素(ET)、血管緊張素(Ang)、醛固酮(ALD)、兒茶酚胺(CA)、白細(xì)胞介素-6(IL-6)、超敏反應(yīng)蛋白(hs-CRP)及環(huán)磷酸鳥苷(c-GMP)含量;取小鼠心臟、腎臟和胸主動(dòng)脈組織制作石蠟切片觀察其形態(tài)。結(jié)果過濾水pH、TDS、OC、EC、鈣和鎂均高于純水和自來水,ORP呈負(fù)值,且溶解氫含量高;模型過濾水組小鼠收縮壓與平均血壓[(106.24±5.31)和(90.73±4.99)mmHg]低于模型對(duì)照組[(119.58±6.08)和(96.44±6.48)mmHg],差異有統(tǒng)計(jì)學(xué)意義(P0.05);模型過濾水組NO含量[(87.05±39.82)μmol/L]明顯高于模型對(duì)照組[(45.01±9.62)μmol/L]和模型自來水組[(46.56±30.54)μmol/L],差異有統(tǒng)計(jì)學(xué)意義(P0.05);模型自來水組和模型過濾水組IL-6[(201.42±36.41)和(173.99±114.96)pg/mL]和Ang含量[(1319.20±111.90)和(1349.38±180.15)ng/L]顯著低于模型對(duì)照組[(363.14±149.00)pg/mL和(1736.17±242.86)ng/L],差異有統(tǒng)計(jì)學(xué)意義(P0.05);模型過濾水組T-SOD和GSH-Px活性[(268.37±12.25)和(712.45±30.59)U/mL]顯著高于模型對(duì)照組[(250.46±15.60)和(678.36±35.80)U/mL],差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論與純水、自來水相比,過濾水可通過抗氧化、抗炎作用降低高血壓小鼠血壓。
[Abstract]:Objective to explore the effect of three kinds of drinking water on blood pressure in hypertensive mice and its possible mechanism. Methods three kinds of drinking water quality were determined: dissolved total solid (TDS), oxygen consumption (OC), p H, redox potential (ORP), conductivity (EC), dissolved hydrogen calcium and magnesium; The hypertensive model of ICR mice was established by continuous intragastric perfusion of Na-nitro-L-arginine methyl ester hydrochloride (L-NAME) for 30 days. Then the mice were randomly divided into four groups: normal control group, model tap water group and model filtered water group. Blood pressure was measured every month and serum nitric oxide (NO), nitric oxide synthase (NOS), was measured after 3 months. Total superoxide dismutase (T-SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), endothelin (ET), angiotensin (Ang), aldosterone (ALD), catecholamine (CA), Interleukin-6 (IL-6), hypersensitive protein (hs-CRP) and cyclic guanosine monophosphate (c-GMP); The hearts, kidneys and thoracic aorta of mice were taken to make paraffin sections to observe their morphology. Results pH,TDS,OC,EC, calcium and magnesium in filtered water were higher than those in pure water and tap water, ORP was negative, and dissolved hydrogen content was high. The systolic blood pressure and mean blood pressure in the model filtered water group [(106.24 鹵5.31) and (90.73 鹵4.99) mmHg] were significantly lower than those in the model control group [(119.58 鹵6.08) and (96.44 鹵6.48) mmHg] (P0.05). The content of NO in model filtered water group [(87.05 鹵39.82) 渭 mol/L] was significantly higher than that in model control group [(45.01 鹵9.62) 渭 mol/L] and model tap water group [(46.56 鹵30.54) 渭 mol/L] (P0.05). IL-6 [(201.42 鹵36.41) and (173.99 鹵114.96) pg/mL] and Ang content [(1319.20 鹵111.90) and (1349.38 鹵180.15) ng/L] in model tap water group and model filtered water group were significantly lower than those in model control group [(201.42 鹵36.41) and (173.99 鹵114.96) pg/mL] [ (363.14 鹵149.00) pg/mL and (1736.17 鹵242.86) ng/L, The difference was statistically significant (P0.05). The activities of T-SOD and GSH-Px in model filtered water group [(268.37 鹵12.25) and (712.45 鹵30.59) U/mL] were significantly higher than those in model control group [(250.46 鹵15.60) and (678.36 鹵35.80) U/mL]. The difference was statistically significant (P0.05). Conclusion compared with pure water and tap water, filtered water can reduce blood pressure of hypertensive mice by anti-oxidation and anti-inflammatory effect.
【作者單位】： 福建醫(yī)科大學(xué)衛(wèi)生檢驗(yàn)與檢疫學(xué)系;廈門大學(xué)分子影像暨轉(zhuǎn)化醫(yī)學(xué)研究中心;
【基金】：福建省高校產(chǎn)學(xué)合作項(xiàng)目(No.2016Y4004)
【分類號(hào)】：R544.1
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本文編號(hào)：2411402