【摘要】：目的:探討miR-125b在頸動脈粥樣硬化(AS)患者外周血單個核細(xì)胞中的表達(dá)及對血管平滑肌細(xì)胞增殖和遷移的影響。方法:選取85例頸AS患者(不穩(wěn)定性斑塊39例,穩(wěn)定性斑塊46例)和45例健康者(對照組),采用實(shí)時熒光定量PCR法檢測外周血單個核細(xì)胞中miR-125b的表達(dá)。培養(yǎng)人主動脈血管平滑肌細(xì)胞,分別轉(zhuǎn)染miR-125b模擬物、miR-125b抑制物、miR-125b陰性對照,以不作任何處理的細(xì)胞為空白對照組,采用實(shí)時熒光定量PCR法檢測各組細(xì)胞中miR-125b的表達(dá),MTT法檢測細(xì)胞增殖活性,Transwell細(xì)胞遷移實(shí)驗(yàn)檢測細(xì)胞遷移能力。結(jié)果:與對照組相比,AS患者外周血單個核細(xì)胞中miR-125b相對表達(dá)量降低,且不穩(wěn)定性斑塊患者低于穩(wěn)定性斑塊患者(P0.05)。miR-125b模擬物組細(xì)胞中miR-125b相對表達(dá)量顯著高于陰性對照組和空白對照組,且miR-125b抑制物組低于陰性對照組和空白對照組(P0.05);miR-125b模擬物組細(xì)胞增殖抑制率和細(xì)胞遷移數(shù)顯著低于陰性對照組和空白對照組,而miR-125b抑制物組則高于陰性對照組和空白對照組(P0.05)。結(jié)論:miR-125b可能通過抑制血管平滑肌細(xì)胞增殖和遷移而發(fā)揮保護(hù)動脈的作用。
[Abstract]:Aim: to investigate the expression of miR-125b in peripheral blood mononuclear cells (PBMC) of patients with carotid atherosclerosis (AS) and its effect on proliferation and migration of vascular smooth muscle cells (VSMC). Methods: 85 patients with cervical AS (39 with unstable plaque and 46 with stable plaque) and 45 healthy subjects (control group) were selected to detect the expression of miR-125b in peripheral blood mononuclear cells by real-time fluorescence quantitative PCR. Cultured human aortic vascular smooth muscle cells were transfected with miR-125b analogue, miR-125b inhibitor, miR-125b negative control, and the cells without any treatment were used as blank control group. The expression of miR-125b was detected by real-time fluorescence quantitative PCR assay, cell proliferation activity was detected by MTT assay, and cell migration ability was detected by Transwell cell migration assay. Results: compared with the control group, the relative expression of miR-125b in peripheral blood mononuclear cells of AS patients was decreased. The relative expression of miR-125b in miR-125b analog group was significantly higher than that in negative control group and blank control group. MiR-125b inhibitor group was lower than negative control group and blank control group (P0.05). The cell proliferation inhibition rate and cell migration number in miR-125b mimic group were significantly lower than those in negative control group and blank control group, while those in miR-125b inhibitor group were higher than those in negative control group and blank control group (P0.05). Conclusion: miR-125b may protect arteries by inhibiting the proliferation and migration of vascular smooth muscle cells.
【作者單位】： 新疆石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院老干部科;新疆石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院神經(jīng)外科;
【基金】：國家自然科學(xué)基金資助項(xiàng)目81360185
【分類號】：R543.4
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本文編號：2411439