原發(fā)免疫性血小板減少癥外周血中T細胞miRNA181a與Treg細胞相關性的分析
發(fā)布時間：2018-12-26 17:41
【摘要】：目的:原發(fā)免疫性血小板減少癥(primary immune thrombocytopenia,ITP),亦稱特發(fā)性血小板減少性紫癜,是臨床上最常見的出血性疾病,臨床上以輕到重度血小板減少,相對表現正常的骨髓像和皮膚、粘膜出血為主要臨床特征,嚴重者可因內臟或顱內出血而危及生命。國內外文獻表明,ITP患者體內存在T細胞亞群失衡,但觸發(fā)ITP中T細胞亞群失衡的發(fā)病機制并不清楚,可明確地是Treg細胞參與ITP發(fā)病。我們通過檢測ITP患者及正常人體內T淋巴細胞mi RNA181a表達水平、Treg細胞及其分泌細胞因子TGF-β水平,來探討mi RNA181a與Treg細胞相關性的分析及兩者在ITP發(fā)病中所起到的作用。方法:1、外周血T淋巴細胞分離2、通過定量PCR對ITP患者、健康對照組外周血T淋巴細胞mi RNA181a表達水平測定。3、通過流式細胞儀檢測ITP患者和健康對照組外周血Treg細胞(CD4+Foxp3+)水平。4、應用ELISA試劑盒檢測ITP患者和健康對照組血漿中TGF-β的水平。5、應用SPSS統(tǒng)計學分析軟件對所得數據進行統(tǒng)計學處理,并分析T細胞mi RNA181a表達與調節(jié)T細胞及其分泌細胞因子相關性結果:1.外周血中T淋巴細胞中m RNA181a的表達量:ITP組:0.495±0.109正常人組:1.296±0.450.ITP組表達量明顯低于正常組,P0.001差異有統(tǒng)計學意義。2.外周血中CD4+T淋巴細胞中Treg細胞(CD4+Foxp3)比例(%):ITP組:1.82±0.46。正常人組:7.21±1.08。ITP組比例明顯低于正常組,P0.05,差異有統(tǒng)計學意義。3.外周血中TGF-β水平:ITP組:3040.1±265.6正常人組:3442.0±276.6。ITP組比例明顯低于正常組,P0.05,差異有統(tǒng)計學意義。4.micro RNA181a與treg細胞相關性分析:micro RNA181a表達水平與treg細胞比例呈正相關,P0.05,r=0.99。結論:1、ITP患者外周血T淋巴細胞mi RNA181a的表達水平下降。2、ITP患者外周血Treg細胞比例及其分泌細胞因子TGF-β降低,說明ITP患者Treg細胞免疫功能受抑。3、ITP患者外周血mi RNA181a表達量與Treg細胞比例呈正相關。4、mi RNA181a表達可能與ITP中發(fā)病機制有關。
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 鹵0.109 normal persons group: 1.296 鹵0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 鹵0.46). Normal group: 7.21 鹵1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 鹵265.6 normal persons group: 3442.0 鹵276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R558.2
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 鹵0.109 normal persons group: 1.296 鹵0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 鹵0.46). Normal group: 7.21 鹵1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 鹵265.6 normal persons group: 3442.0 鹵276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R558.2
【參考文獻】
相關期刊論文 前7條
1 李慧媛;張冬雷;張峴;付榮鳳;宣e，
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