原發(fā)免疫性血小板減少癥外周血中T細(xì)胞miRNA181a與Treg細(xì)胞相關(guān)性的分析
發(fā)布時(shí)間：2018-12-26 17:41
【摘要】：目的:原發(fā)免疫性血小板減少癥(primary immune thrombocytopenia,ITP),亦稱特發(fā)性血小板減少性紫癜,是臨床上最常見(jiàn)的出血性疾病,臨床上以輕到重度血小板減少,相對(duì)表現(xiàn)正常的骨髓像和皮膚、粘膜出血為主要臨床特征,嚴(yán)重者可因內(nèi)臟或顱內(nèi)出血而危及生命。國(guó)內(nèi)外文獻(xiàn)表明,ITP患者體內(nèi)存在T細(xì)胞亞群失衡,但觸發(fā)ITP中T細(xì)胞亞群失衡的發(fā)病機(jī)制并不清楚,可明確地是Treg細(xì)胞參與ITP發(fā)病。我們通過(guò)檢測(cè)ITP患者及正常人體內(nèi)T淋巴細(xì)胞mi RNA181a表達(dá)水平、Treg細(xì)胞及其分泌細(xì)胞因子TGF-β水平,來(lái)探討mi RNA181a與Treg細(xì)胞相關(guān)性的分析及兩者在ITP發(fā)病中所起到的作用。方法:1、外周血T淋巴細(xì)胞分離2、通過(guò)定量PCR對(duì)ITP患者、健康對(duì)照組外周血T淋巴細(xì)胞mi RNA181a表達(dá)水平測(cè)定。3、通過(guò)流式細(xì)胞儀檢測(cè)ITP患者和健康對(duì)照組外周血Treg細(xì)胞(CD4+Foxp3+)水平。4、應(yīng)用ELISA試劑盒檢測(cè)ITP患者和健康對(duì)照組血漿中TGF-β的水平。5、應(yīng)用SPSS統(tǒng)計(jì)學(xué)分析軟件對(duì)所得數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)處理,并分析T細(xì)胞mi RNA181a表達(dá)與調(diào)節(jié)T細(xì)胞及其分泌細(xì)胞因子相關(guān)性結(jié)果:1.外周血中T淋巴細(xì)胞中m RNA181a的表達(dá)量:ITP組:0.495±0.109正常人組:1.296±0.450.ITP組表達(dá)量明顯低于正常組,P0.001差異有統(tǒng)計(jì)學(xué)意義。2.外周血中CD4+T淋巴細(xì)胞中Treg細(xì)胞(CD4+Foxp3)比例(%):ITP組:1.82±0.46。正常人組:7.21±1.08。ITP組比例明顯低于正常組,P0.05,差異有統(tǒng)計(jì)學(xué)意義。3.外周血中TGF-β水平:ITP組:3040.1±265.6正常人組:3442.0±276.6。ITP組比例明顯低于正常組,P0.05,差異有統(tǒng)計(jì)學(xué)意義。4.micro RNA181a與treg細(xì)胞相關(guān)性分析:micro RNA181a表達(dá)水平與treg細(xì)胞比例呈正相關(guān),P0.05,r=0.99。結(jié)論:1、ITP患者外周血T淋巴細(xì)胞mi RNA181a的表達(dá)水平下降。2、ITP患者外周血Treg細(xì)胞比例及其分泌細(xì)胞因子TGF-β降低,說(shuō)明ITP患者Treg細(xì)胞免疫功能受抑。3、ITP患者外周血mi RNA181a表達(dá)量與Treg細(xì)胞比例呈正相關(guān)。4、mi RNA181a表達(dá)可能與ITP中發(fā)病機(jī)制有關(guān)。
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 鹵0.109 normal persons group: 1.296 鹵0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 鹵0.46). Normal group: 7.21 鹵1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 鹵265.6 normal persons group: 3442.0 鹵276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R558.2
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 鹵0.109 normal persons group: 1.296 鹵0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 鹵0.46). Normal group: 7.21 鹵1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 鹵265.6 normal persons group: 3442.0 鹵276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R558.2
【參考文獻(xiàn)】
相關(guān)期刊論文 前7條
1 李慧媛;張冬雷;張峴;付榮鳳;宣e，
本文編號(hào)：2392461
本文鏈接：http://sikaile.net/yixuelunwen/xxg/2392461.html
最近更新
教材專著
