【摘要】：目的：探討兔急性心肌梗死不同時間,血清一氧化氮濃度的動態(tài)變化,及其與肌酸激酶同工酶(CK-MB)、心肌肌鈣蛋白T (cTnT)的關系；心肌梗死不同時間后再灌注60分鐘時,血清一氧化氮濃度的變化。方法：42只成年健康新西蘭大白兔,隨機分成為A、B、C、D、E、F組,每組6只。通過結扎兔冠狀動脈左前降支,制作兔急性心肌梗死模型,檢測不同時間點血清CK-MB、CTnT及NO的濃度。結扎不同時間后,通過松扎左側冠狀動脈前降支60分鐘,制作兔心肌缺血再灌注模型,檢測松扎后60分鐘時血清CK-MB、CTnT及NO的濃度,并全程記錄心電圖的變化。A組結扎5分鐘后松扎60分鐘(n=6)、B組結扎10分鐘后松扎60分鐘(n=6)、C組結扎30分鐘后松扎60分鐘(n=6)、D組結扎1小時后松扎60分鐘(n=6)、E組結扎3小時后松扎60分鐘(n=6)、F組結扎6小時后松扎60分鐘(n=6),并設假手術組作為對照(n=6)。檢測不同時間點血清CK-MB、CTnT及NO的濃度。檢測松扎后60分鐘時結扎兔冠狀動脈前降支5分鐘、10分鐘、30分鐘,1小時、3小時、6小、12小時、24小時和松扎后,記錄結扎前后一氧化氮變化及心電圖。應用Biovision一氧化氮檢測分析試劑盒(比色法),兔肌酸激酶同工酶(CK-MB) ELISA檢測試劑盒(上海),兔肌鈣蛋白T(CTnT)ELISA檢測試劑盒(上海)。用南京建成生物工程研究所提供的CK-MB、CTnT及NO試劑盒,比色法測定各標本血漿中的CK-MB、CTnT及NO的濃度或活力。各項數據均用均數±標準差(i±s)表示。組間比較用單因素方差分析,組內比較用配對T檢驗。(P0.05)為具有顯著性差異。結果：1.CK-MB、CTnT及NO組間比較P0.05,差異有統(tǒng)計學意義。2.各實驗組組內比較差異具有統(tǒng)計學意義(P0.05)。3.心電圖：結扎后再通的急性心肌梗死兔模型再灌注損傷性心律失常有較高的發(fā)生率。與無再灌注損傷性心電圖改變組比較,再灌注損傷性心電圖改變組血清NO顯著降低(P0.01)。結論：1.兔的胸腔結構特殊,給實驗操作帶來很多方便。兔子大小、價格適中,心腔結構與人類相似,利于實驗和效果的評價,適合用于心肌缺血再灌注模型。2.兔AMI模型NO水平顯著降低、再灌注不能改善AMI模型的NO降低。3.NO是有可能是早于心電圖、血清CK-MB和cTnT診斷AMI的血清標記物。4.血清NO濃度與心肌梗死ST段的抬高與下降之間存在關聯,為今后進一步的研究提供依據。
[Abstract]:Objective: to investigate the dynamic changes of serum nitric oxide (no) concentration and its relationship with creatine kinase isoenzyme (CK-MB) and cardiac troponin T (cTnT) in rabbits with acute myocardial infarction (AMI) at different time and 60 minutes after reperfusion. Changes of serum nitric oxide concentration. Methods: 42 healthy adult New Zealand rabbits were randomly divided into two groups (6 in each group). A rabbit model of acute myocardial infarction was made by ligating the left anterior descending branch of coronary artery. Serum CK-MB,CTnT and NO were measured at different time points. After different time of ligation, the left anterior descending coronary artery was ligated for 60 minutes to make the myocardial ischemia-reperfusion model in rabbits. The serum CK-MB,CTnT and NO concentrations were measured at 60 minutes after ligation. Electrocardiogram changes were recorded in the whole course. Group A was ligated for 5 minutes and then loosened for 60 minutes (nong6), B group was ligated for 10 minutes and then loosened for 60 minutes (nong6), C group for 30 minutes after ligation for 30 minutes) (nun6), D group for 1 hour after ligation for 1 hour) (nun6), E group for 3 hours). Posterior ligation was performed 60 minutes after ligation for 6 hours in nong6), F group and sham operation group was used as control group. Serum CK-MB,CTnT and NO concentrations were measured at different time points. The changes of nitric oxide (no) and electrocardiogram (ECG) before and after ligation of anterior descending branch of coronary artery in rabbits were measured 60 minutes after ligation for 5 minutes, 10 minutes, 30 minutes, 1 hour, 3 hours, 6 hours, 12 hours, 24 hours and after ligation. Biovision nitric oxide assay kit (colorimetric method), rabbit creatine kinase isoenzyme (CK-MB) ELISA assay kit (Shanghai) and rabbit troponin T (CTnT) ELISA assay kit (Shanghai) were used. The concentration and activity of CK-MB,CTnT and NO in plasma were determined by colorimetric method with CK-MB,CTnT and NO kit provided by Nanjing Institute of Bioengineering. All the data were expressed as mean 鹵standard deviation (I 鹵s). Single factor analysis of variance (ANOVA) was used in the comparison between groups, and T test was used in intra-group comparison. (P0.05) there was significant difference. Results: 1. There was significant difference between CK-MBN CTnT and NO group (P 0.05). There were significant differences among the experimental groups (P0.05). Electrocardiogram (ECG): reperfusion injury arrhythmias have a high incidence in rabbit models of acute myocardial infarction after ligation. Compared with the group without reperfusion injury, the serum NO was significantly lower in the reperfusion injury group than in the no reperfusion injury group (P0.01). Conclusion 1. The special structure of the rabbit chest cavity brings a lot of convenience to the experimental operation. The size and price of rabbits are moderate, and the structure of heart cavity is similar to that of human beings. It is suitable for myocardial ischemia-reperfusion model. 2. The level of NO in rabbit AMI model was significantly decreased, but reperfusion could not improve the decrease of NO in AMI model. 3.NO might be the serum marker for diagnosis of AMI in serum CK-MB and cTnT earlier than ECG. 4. There is a correlation between serum NO concentration and elevation and decrease of ST segment in myocardial infarction, which provides basis for further study.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R542.22
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本文編號：2275571