抗β2GPI抗體促進(jìn)載脂蛋白E缺陷小鼠血管炎癥及血栓相關(guān)分子的表達(dá)
[Abstract]:Objective to investigate the role of anti 尾 2 glycoprotein I (尾 2GPI) antibody in the expression of atherosclerotic inflammatory factors and thrombus related molecules in apolipoprotein E deficient mice. Methods ApoE- /-mice were randomly divided into 100 渭 g anti 尾 2GPI antibody group (rabbit IgG) and 100 渭 g 尾 2GPI/ anti 尾 2GPI antibody complex group. Each group was given a high fat diet and the mice were killed after 6 weeks of intraperitoneal injection of the corresponding stimulator every 7 days. The occlusion of carotid artery was observed by HE staining. The expression of TLR4, tissue factor (TF) and von Willebrand factor (vWF) were detected by immunohistochemical staining. The mRNA levels of interleukin 1 尾 (IL-1 尾) and tumor necrosis factor 偽 (TNF- 偽) in aorta were measured by real-time fluorescence quantitative PCR. Results HE staining showed that the blocking was the most obvious in the antibody group, and the expression of TLR4TF and vWF in the carotid artery of the antibody group was up-regulated. The mRNA levels of IL-1 尾 and TNF- 偽 in the aorta of the antibody group were significantly higher than those of the other three groups. Conclusion Anti-beta 2GPI can promote the formation of atherosclerotic plaques by up-regulating the expression of inflammatory cytokines IL-1 尾 and TNF- 偽, thrombus related molecules TF, vWF and TLR4.
【作者單位】: 江蘇大學(xué)醫(yī)學(xué)院;
【基金】:國家自然科學(xué)基金(81370614)
【分類號】:R54
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