本文選題：自組裝短肽 + GFS-　； 參考：《生物醫(yī)學(xué)工程學(xué)雜志》2017年03期

【摘要】：本研究探索自組裝短肽GFS-4在心肌細胞三維培養(yǎng)中的應(yīng)用效果及其對心肌梗死區(qū)域的組織修復(fù)作用。通過圓二色譜儀分析短肽GFS-4的二級結(jié)構(gòu),原子力顯微鏡檢測短肽GFS-4自組裝的微觀形態(tài)。將GFS-4自組裝形成的納米纖維支架作為心肌細胞三維培養(yǎng)材料,觀察心肌細胞的生長狀況;建立大鼠心肌梗死模型,加入水凝膠GFS-4研究其對心肌梗死修復(fù)的效果。結(jié)果發(fā)現(xiàn),GFS-4自組裝形成的二級結(jié)構(gòu)主要為β折疊;自組裝24 h后形成致密的納米纖維支架;心肌細胞三維培養(yǎng)結(jié)果表明心肌細胞在GFS-4水凝膠中生長狀況良好;心肌梗死體外修復(fù)實驗發(fā)現(xiàn),短肽GFS-4水凝膠支架可緩解心肌梗死區(qū)域組織壞死。自組裝短肽GFS-4作為新的納米支架材料,可用于細胞三維培養(yǎng)和心肌梗死區(qū)域組織修復(fù)。
[Abstract]:The purpose of this study was to investigate the effect of self assembled short peptide GFS-4 in three dimensional culture of cardiomyocytes and its effect on tissue repair in myocardial infarction area. The secondary structure of short peptide GFS-4 was analyzed by circular dichroism chromatograph. Atomic force microscope (AFM) was used to detect the microstructure of short peptide GFS-4 self assembly. GFS-4 self-assembled nano-fiber scaffold was used as a three-dimensional culture material to observe the growth of cardiac myocytes and to establish myocardial infarction model in rats. The effect of hydrogel GFS-4 on myocardial infarction repair was studied by adding hydrogel GFS-4. The results showed that the secondary structure of GFS-4 was mainly 尾 -folding, compact nano-fiber scaffold was formed 24 h after self-assembly, and the results of three-dimensional culture of cardiomyocytes showed that the growth of cardiomyocytes in GFS-4 hydrogel was good. In vitro repair of myocardial infarction, short peptide GFS-4 hydrogel stent can alleviate the necrosis of myocardial infarction area. Self-assembled peptide GFS-4 as a new nano-scaffold material can be used in three-dimensional cell culture and tissue repair in myocardial infarction area.
【作者單位】： 重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院分子醫(yī)學(xué)與腫瘤研究中心;鄭州人民醫(yī)院心血管內(nèi)科第三病區(qū);上海交通大學(xué)醫(yī)學(xué)院附屬新華醫(yī)院心血管內(nèi)科;樂山市婦幼保健院檢驗科;
【基金】：國家自然科學(xué)基金(31540019) 重慶市科委自然科學(xué)基金(cstc2015jcyj BX0072) 重慶醫(yī)科大學(xué)科研培育基金(自然科學(xué)類)(201417);重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院苗圃基金(JC201514) 四川省重點實驗室抗生素及其相關(guān)研究實驗室基金(ARRLKF14-01)
【分類號】：R318.08;R542.22

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1 袁霄;構(gòu)建自組裝肽納米纖維水凝膠用于心肌修復(fù)[D];重慶醫(yī)科大學(xué);2016年

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