發(fā)布時間：2018-03-21 15:32

  本文選題：彈力蛋白　切入點(diǎn)：動脈粥樣硬化　出處：《浙江大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 對比非冠狀動脈粥樣硬化性心臟病患者與冠狀動脈粥樣硬化性心臟病(冠心病)患者,以及不同類型的冠心病患者(穩(wěn)定型心絞痛、不穩(wěn)定型心絞痛與急性心肌梗死)中血清彈力蛋白的水平,分析血清彈力蛋白水平與冠狀動脈粥樣硬化性心臟病的相關(guān)性。 方法 連續(xù)選取在浙江大學(xué)附屬第二醫(yī)院心內(nèi)科住院行冠狀動脈造影的患者共253例,其中經(jīng)冠狀動脈造影明確診斷非冠心病患者96例,冠心病患者157例。收集所有入選患者的基線臨床資料包括性別、年齡、糖尿病、高血壓病、吸煙情況、體重指數(shù)等。入院后第二天清晨至少空腹8小時后抽取肘靜脈血檢測常規(guī)生化指標(biāo)包括：空腹血糖、總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)等,并留取血清標(biāo)本凍存于-80℃待集中檢測。用ELISA試劑盒測定樣本的血清彈力蛋白水平。各組間進(jìn)行單因素分析,將P0.05的變量納入回歸模型,多元線性回歸分析彈力蛋白水平與性別、年齡等變量的關(guān)系。 結(jié)果 與非冠心病組比較,冠心病患者組年齡、性別、高密度脂蛋白、血清彈力蛋白均有統(tǒng)計(jì)學(xué)意義(P0.05),但兩組在體重指數(shù)、空腹血糖、總膽固醇、甘油三酯、低密度脂蛋白、吸煙、高血壓、糖尿病等均無統(tǒng)計(jì)學(xué)差異(P0.05)。各亞組患者與非冠心病患者臨床基本資料的差異主要體現(xiàn)在年齡、體重指數(shù)以及性別(P0.05)；而在空腹血糖、總膽固醇、甘油三酯、HDL、LDL、吸煙、高血壓、糖尿病均無明顯顯著性差異(P0.05)。而所有入組患者的血清彈力蛋白含量與甘油三酯濃度、性別以及吸煙情況相關(guān)。將與冠心病在統(tǒng)計(jì)學(xué)上有顯著性差異的變量以及與血清彈力蛋白表達(dá)相關(guān)的變量應(yīng)用逐步選擇法進(jìn)行多元線性回歸,發(fā)現(xiàn)血清彈力蛋白濃度在同性別的冠心病患者中濃度明顯偏高(P0.05),而在冠心病不同階段并未發(fā)現(xiàn)顯著差異。 結(jié)論 血清彈力蛋白水平在冠心病患者較非冠心病患者顯著升高,但其與冠心病的類型無相關(guān)性。
[Abstract]:Purpose. Comparison was made between patients with non-coronary atherosclerotic heart disease and patients with coronary atherosclerotic heart disease (coronary heart disease) and patients with different types of coronary heart disease (stable angina pectoris). The levels of serum elastin in unstable angina pectoris and acute myocardial infarction (AMI) and the correlation between serum elastin levels and coronary atherosclerotic heart disease (CHD) were analyzed. Method. A total of 253 consecutive patients were selected for coronary angiography in the Department of Cardiology, affiliated to the second affiliated Hospital of Zhejiang University, of which 96 were definitely diagnosed by coronary angiography as non-coronary heart disease patients. 157 patients with coronary heart disease. Baseline clinical data including gender, age, diabetes, hypertension, smoking were collected. Body mass index (BMI). The following morning, at least 8 hours after admission, blood samples from elbow veins were taken to detect routine biochemical parameters such as fasting blood glucose, total cholesterol TCU, triglyceride TGN, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and so on. The serum samples were frozen at -80 鈩，

本文編號：1644470