本文選題：交感神經(jīng)系統(tǒng)　切入點(diǎn)：心房顫動(dòng)　出處：《中國循環(huán)雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的:通過建立擬交感性心房顫動(dòng)(房顫)模型,探討心肌縫隙連接蛋白(Cx)43和Cx40表達(dá)水平的變化。方法:選擇雜種犬15只,隨機(jī)分為3組,即:對(duì)照組(N組)、快速心房起搏組(RAP組)和快速心房起搏+異丙腎上腺素(ISO)灌流組(RAP+ISO組),每組5只,處死后取出心臟,建立langendorff心臟離體灌流模型。3組分別檢測(cè)心房有效不應(yīng)期(AERP)及房顫誘發(fā)率,免疫組化檢測(cè)神經(jīng)生長因子、酪氨酸羥化酶在細(xì)胞內(nèi)的表達(dá)及分布,蛋白免疫印記法檢測(cè)Cx43和Cx40總蛋白水平,透射電鏡檢測(cè)線粒體形態(tài),熒光比色法檢測(cè)線粒體內(nèi)活性氧簇(ROS)生成量。結(jié)果:與N組AERP[(166±5.1)ms]比較,RAP組AERP[(160±3.2)ms]無明顯改變,無法誘發(fā)出房顫,RAP+ISO組AERP[(148±3.7)ms]明顯縮短(P0.05),并可成功誘發(fā)房顫。與N組比較,RAP組線粒體輕度腫脹,基質(zhì)基本完整,RAP+ISO組線粒體明顯腫脹,部分基質(zhì)透明。RAP組和RAP+ISO組Cx43和Cx40總蛋白含量均低于N組(P0.05),且RAP+ISO組蛋白含量低于RAP組(P0.05)。RAP+ISO組神經(jīng)生長因子、酪氨酸羥化酶分布和含量以及線粒體內(nèi)ROS生成量顯著高于RAP組和N組(P0.05),而RAP組則高于N組(P0.05),差異均有統(tǒng)計(jì)學(xué)意義。結(jié)論:交感性房顫的發(fā)生與Cx43和Cx40蛋白含量的變化有關(guān),交感神經(jīng)可能通過氧化應(yīng)激下調(diào)Cx43和Cx40的蛋白含量來介導(dǎo)房顫的發(fā)生。
[Abstract]:Objective: to investigate the changes of the expression of gap junction protein (CxCX) 43 and Cx40 in myocardium by establishing a sympathetic atrial fibrillation (AF) model. Methods: fifteen mongrel dogs were randomly divided into 3 groups. That is, the control group (n group), the rapid atrial pacing group (Rap group) and the rapid atrial pacing group (isoproterenol group) were perfused with rap ISO group (5 rats in each group), and the hearts were taken out after death. In group 3, the atrial effective refractory period (AERP) and the induced rate of atrial fibrillation were measured. The expression and distribution of nerve growth factor (NGF) and tyrosine hydroxylase (tyrosine hydroxylase) were detected by immunohistochemistry. The total protein levels of Cx43 and Cx40 were detected by protein imprinting, the morphology of mitochondria was detected by transmission electron microscope, and the amount of reactive oxygen species (Ros) in mitochondria was detected by fluorescence colorimetry. Results: there was no significant change in AERP [160 鹵3.2 Ms] in rap group compared with AERP [166 鹵5.1 Ms] in N group. The AERP of rap ISO group [148 鹵3.7 Ms] was significantly shortened and atrial fibrillation could be induced successfully. Compared with group N, the mitochondria of rap group were slightly swollen, and the mitochondria of rap ISO group were basically intact. The total protein contents of Cx43 and Cx40 in some matrix hyaline. Rap and RAP ISO groups were lower than those in N group and RAP ISO group were lower than that in RAP group. The distribution and content of tyrosine hydroxylase and the amount of ROS in mitochondria were significantly higher than those in RAP and N groups (P 0.05N), while those in RAP group were higher than those in N group (P 0.05). Conclusion: the occurrence of sympathetic atrial fibrillation is related to the changes of Cx43 and Cx40 protein levels. Sympathetic nerve may mediate the occurrence of atrial fibrillation through oxidative stress down-regulating the protein content of Cx43 and Cx40.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院老年心內(nèi)科;廣西醫(yī)科大學(xué)第一附屬醫(yī)院心血管病研究所;廣西醫(yī)科大學(xué)第一附屬醫(yī)院心胸外科;
【基金】：國家自然科學(xué)基金(81260039) 廣西自然科學(xué)基金(2013GXNSFAA278005) 第一批廣西醫(yī)學(xué)高層次骨干人才培養(yǎng)“139”計(jì)劃經(jīng)費(fèi)
【分類號(hào)】：R-332;R541.75

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