本文關(guān)鍵詞： 冠心病 GP78 單核苷酸多態(tài)性 病例對(duì)照研究　出處：《新疆醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討GP78基因多態(tài)性與冠心病的相關(guān)性。方法：選擇維吾爾族和漢族兩個(gè)人群分別進(jìn)行病例對(duì)照研究,漢族人群：選擇經(jīng)冠脈造影檢查確診的冠心病患者602例和性別、年齡、民族均匹配的正常健康對(duì)照人群572例；維族人群：選擇經(jīng)冠脈造影檢查確診的冠心病患者374例和性別、年齡、民族均匹配的正常健康對(duì)照人群376例,選擇GP78基因的3個(gè)SNPs (rs731119, rs2617849 and rs2440472),采用實(shí)時(shí)熒光定量PCR (TaqMan)方法對(duì)GP78基因進(jìn)行基因分型,數(shù)據(jù)分為三組：整體組、男性組,女性組。結(jié)果：在漢族人群中,對(duì)于整體,GP78基因的rs2440472的基因型分布在冠心病組和健康對(duì)照組間有顯著差異(P=0.008),對(duì)于整體組和男性組,SNP3 (rs2440472)的等位基因分布、顯性模型(AA VS AG+GG)以及隱性模型(GG VS AG+AA)在冠心病組和健康對(duì)照組之間有顯著差異(等位基因：P=0.003vsP=0.002；顯性模型：P=0.041vsP=0.026；隱形模型：P=0.004vs0.004)。在調(diào)整‘主要危險(xiǎn)因素的干擾后,Logistic回歸分析顯示：對(duì)于整體組和男性組,顯性模型(AA VS AG+GG)和隱形模型(GG VS AG+AA)分布在冠心病組和健康對(duì)照組間仍然均有顯著差異(顯性模型整體：OR:0.760,95% CI:0.584-0.99, P=0.042;顯性模型男性組：OR:0.686,95% CI:0.498-0.946, P=0.022;隱形模型整體OR:1.451,95% CI:1.067-1.974, P=0.018;隱形模型男性組：OR:1.789,95% CI:1.219-2.627).在維吾爾族人群中,GP78基因的3個(gè)SNPs (rs731119, rs2617849 and rs2440472)的基因型及等位基因分布頻率在冠心病組和對(duì)照組間均無顯著差異(P均0.05)。結(jié)論：GP78基因rs2440472的GG基因型和G等位基因可能是漢族人群發(fā)生冠心病的危險(xiǎn)因素,可做為冠心病的易感基因標(biāo)記。而AA基因型和A等位基因可能是漢族人群發(fā)生冠心病的保護(hù)因素。
[Abstract]:Objective: to investigate the relationship between GP78 gene polymorphism and coronary heart disease (CHD). Methods: a case-control study was conducted in two populations of Uygur and Han nationality. 572 healthy controls matched by age and nationality, 374 patients with coronary heart disease confirmed by coronary angiography, 376 healthy controls matched by age and nationality were selected. Three SNPs rs731119 and rs2617849 and rs2440472 of GP78 gene were selected, and the genotyping of GP78 gene was carried out by real-time fluorescent quantitative PCR Taq Man. the data were divided into three groups: whole group, male group and female group. There was a significant difference in the genotype distribution of rs2440472 between CHD group and healthy control group, and the allele distribution of SNP3 rs2440472 in whole group and male group. There were significant differences between the dominant model (AA vs AG GGG) and the recessive model (GG vs AG AAA) between the coronary heart disease group and the healthy control group (allele: 1: 0. 003 vs P0. 002; the dominant model: P0. 041 vs P0. 026; the invisible model: P0. 004 vs 0. 004. after adjusting for the interference of 'main risk factors), logistic regression analysis was performed. For the whole group and the male group, There were still significant differences in the distribution of AA-VS AG GGG and GG VS AG AAA between coronary heart disease group and healthy control group (dominant model: OR0.76095% CI: 0.584-0.99, P0. 042; dominant male group: 0. 68695% CIW 0. 498-0.946, P0. 022; occult model: OR1.45195%: CI1.067-1.974, Pu. 0.018; male: ORW: 0. 68695% CIW 0. 498-0.946; occult model: OR1.45195%: CI1.067-1.974, P0. 018; male: 1. 68695% CI: 0. 498-0. 022; There were no significant differences in genotype and allele frequencies of three SNPs rs731119 and rs2617849 and rs2440472 of GP78 gene between coronary heart disease group and control group. Conclusion there is no significant difference in GG genotype and allele frequency between coronary heart disease group and control group. Conclusion the GG genotypes and alleles of rs2617849 GP78 gene rs2440472 have no significant difference between coronary heart disease group and control group. Conclusion there is no significant difference in GG genotype and allele frequency between coronary heart disease group and control group. G allele may be the risk factor of coronary heart disease in Han population. AA genotype and A allele may be the protective factors of coronary heart disease in Han population.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R541.4
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本文編號(hào)：1516027