【背景及目的】門脈高壓是使肝硬化病人死亡風(fēng)險(xiǎn)增高的重要因素之一。實(shí)驗(yàn)性肝硬化大鼠肝臟中RhoA及Rho-kinase表達(dá)水平明顯高于正常大鼠，RhoA/Rho-kinase信號(hào)通道的激活導(dǎo)致血管平滑肌收縮并抑制其舒張，從而促進(jìn)了門脈高壓的發(fā)生。相關(guān)研究報(bào)道，阿魏酸能夠降低膽固醇合成，后者合成過程的中間產(chǎn)物GGPP是RhoA活化的關(guān)鍵物質(zhì)。本實(shí)驗(yàn)旨在研究阿魏酸對(duì)肝硬化大鼠門脈壓力的作用，并探討相關(guān)分子機(jī)制�！痉椒ā坑媚懝芙Y(jié)扎方法制作肝硬化大鼠模型。實(shí)驗(yàn)動(dòng)物分為SHAM+NS、BDL+NS及BDL+SF三組，比較分析各組大鼠大體特征及血清肝功能指標(biāo)。阿魏酸治療對(duì)肝臟纖維化程度的影響通過顯微鏡下觀察肝臟HE染色切片、透射電鏡觀察肝臟超微結(jié)構(gòu)改變，以及免疫組化檢測(cè)肝臟a-SMA表達(dá)等綜合評(píng)定。免疫組化方法檢測(cè)各組大鼠肝臟中RhoA、Rho-kinase及eNOS的表達(dá)。分離、培養(yǎng)原代大鼠HSC，另購(gòu)入人肝星狀細(xì)胞系LX-2，分別給予阿魏酸/阿魏酸+GGPP處理，流式細(xì)胞術(shù)檢測(cè)各處理因素作用下細(xì)胞凋亡率。原位肝灌注方法測(cè)定大鼠原位肝灌注壓力/肝內(nèi)血管阻力，以及由低到高濃度梯度的甲氧明對(duì)各組大鼠肝... 

【文章來源】：華中科技大學(xué)湖北省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：57 頁(yè)

【學(xué)位級(jí)別】：碩士

【部分圖文】：

阿魏酸納經(jīng)RhoA/ROCK通路對(duì)肝硬化門脈壓力的作用及機(jī)制分析

光鏡下觀察各組大鼠肝臟HE染色切片

電鏡下觀察各組大鼠肝臟超微結(jié)構(gòu)

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