發(fā)布時間：2021-10-07 18:44

　　目的用�；撬幔═aurine）對重癥急性胰腺炎（severe acute pancreatitis ,SAP）大鼠進行預處理,采用聯(lián)合酶原位灌注法分離枯否細胞（Kupffer cells,KCs）,檢測KCs中P38-絲裂原蛋白激酶（P38 mitogen activated protein kinase, P38MAPK）的蛋白量和其活化水平以及核因子-κB（Nuclear factor-kappaB, NF-κB）的活性,探討�；撬釋χ匕Y急性胰腺炎大鼠KCs內P38MAPK的影響以及對分泌促炎細胞因子的作用。方法48只雄性SD大鼠采用完全隨機法分為:①假手術對照組（sham operation,SO）;②重癥急性胰腺炎組（SAP）;③牛磺酸預處理組（Tau）。SAP模型通過胰膽管逆行注射5%�；悄懰徕c誘導,造模前Tau組分別于24h和12h從尾靜脈內注入�；撬幔�300mmol/L,1.0ml /100g）,其它組注入等量的生理鹽水。假手術或造模后分別于12h和24h處死動物,檢測其血清中AST、ALT和AMS含量;然后采用聯(lián)合酶原位灌注法分離KCs,采用蛋白免疫印跡法和免疫細胞化學... 

【文章來源】：重慶醫(yī)科大學重慶市

【文章頁數(shù)】：55 頁

【學位級別】：碩士

【部分圖文】：

各時間點大鼠血清中AST水平的變化

圖 1-1：各時間點大鼠血清中 AST 水平的變化注： ﹡與 SO 組比較，P<0.01； ﹟與 SAP 組比較，P<0.05。Fig 1-1: The change of AST at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.

圖 1-3：各時間點大鼠血清中AMS水平的變化注： ﹡與 SO 組比較，P<0.01； ﹟與 SAP 組比較，P<0.05。Fig 1-3 :The change of AMS at every time in three group rats﹡P<0.01 vs SO group; ﹟P<0.05 vs SAP group.大鼠KCs中P38MAPK的活化情況2所示：雖然三組大鼠各時間點KCs中相對的P38MAPK的蛋白量.05），但各時間點SAP組大鼠KCs中P38MAPK明顯被活化為，其明顯增高，與SO組比較，差異顯著(P<0.01)。用�；撬犷A處理后大下降，與SAP組相比，差異有統(tǒng)計學意義(P<0.05)。 2 KCs 中 P38MAPK 的蛋白量及磷酸化水平(P38MAPK/β-actin) ( )Tab2 The proteinum and phosphorylation of P38MAPK in KCs( ) SO 組 SAP 組 Tau 組12h 24h 12h 24h 12h 24h0.8399± 0.8447± 0.7539± 0.6021± 0.7506± 0.8

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