發(fā)布時間：2021-07-21 14:20

　　目的建立骨髓間充質(zhì)干細胞體外培養(yǎng)及擴增體系的基礎(chǔ)上,探討不同時期移植BMSC、移植時間長短以及移植途徑不同對大鼠肝纖維化進程的影響:從TGF-β1的角度出發(fā),探討B(tài)MSC移植在肝纖維化進程中的作用機制以及可能在肝硬化后肝癌發(fā)生中的影響。方法1、應用密度梯度離心結(jié)合貼壁培養(yǎng)法分離純化雄性SD大鼠BMSC,分別向成骨細胞,成脂肪細胞誘導分化進行鑒定。2、在四氯化碳誘導大鼠肝纖維化模型的第六周、第八周,將來自雄性SD大鼠的BMSC以5×10⁶/只的劑量經(jīng)尾靜脈注入同種雌性大鼠體內(nèi)。分別在BMSC移植4周及6周后處死各組大鼠,評價干預組與對照組的生存狀態(tài),并用Scheuer炎癥活動度評分法和Schmid M纖維化評分法評價肝臟病理情況。3、在四氯化碳誘導大鼠肝纖維化模型的第六周,將來自雄性SD大鼠的BMSC以5×10⁶/只的劑量經(jīng)腹腔、經(jīng)尾靜脈及肝內(nèi)注射三種途徑移植入同種雌性大鼠體內(nèi)。BMSC移植6周后分別處死各組大鼠,評價各組的生存狀態(tài),異形細胞發(fā)生率,Scheuer炎癥活動度評分法和Schmid M纖維化評分法評價肝臟病理情況,免疫組化法檢測α-... 

【文章來源】：福建醫(yī)科大學福建省

【文章頁數(shù)】：100 頁

【學位級別】：博士

【部分圖文】：

Btl組肝臟)

【參考文獻】：
期刊論文
[1]自體骨髓干細胞移植治療慢性重癥肝病臨床研究[J]. 姚鵬,胡大榮,王帥,胡學玲,聞煒,周一鳴,龔麗娟.  透析與人工器官. 2006(01)
[2]經(jīng)肝動脈骨髓干細胞移植治療肝硬化的初步臨床應用[J]. 張強,李京雨,徐力揚,劉明,姚鵬,王帥.  中國介入影像與治療學. 2005(04)
[3]Bone marrow-derived mesenchymal stem cells protect against experimental liver fibrosis in rats[J]. Dong-Chang Zhao, Rui Chen, Wei-Hua Yu, Xiu-Ming Zhang, Shu-Nong Li, Peng Xiang, Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, 74# Zhongshan Road II, Guangzhou 510080, Guangdong Province, China Dong-Chang Zhao, Jun-Xia Lei, Wei-Hua Yu, Xiu-Ming Zhang, Shu-Nong Li, Department of Pathophysiology, Medical School of Sun Yat-Sen University, 74# Zhongshan Road II, Guangzhou 510080, Guangdong Province, China Dong-Chang Zhao, Department of Pediatrics, Guangdong Province Maternal and Children’s Hospital, 13# Guangyuan Xilu, Guangzhou 510010. Guanedone Province. China.  World Journal of Gastroenterology. 2005(22)
[4]Effects of Guiyuanfang and autologous transplantation of bone marrow stem cells on rats with liver fibrosis[J]. Li-Mao Wu, Lecturer, Institute of Traditional Chinese Materia Medica, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, Zhejiang Province, China Lian-Da Li, Academician, Affiliated Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China Hong Liu, Postdoctoral, Guangzhou University of Traditional Chinese Medicine, Guangzhou 501405, Guangdong Province, China Ke-Yong Ning, Doctoral Student, Basic Medicine Group, Affiliated Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China Yi-Kui Li, Doctoral Student, Basic Medicine Group, Affiliated Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China.  World Journal of Gastroenterology. 2005(08)
[5]骨髓干細胞在大鼠肝纖維化形成環(huán)境中的分化[J]. 展玉濤,魏來,陳紅松,叢旭,費然,王宇.  中華肝臟病雜志. 2003(11)
[6]TGF-β1-promoted epithelial-to-mesenchymal transformation and cell adhesion contribute to TGF-β1-enhanced cell migration in SMMC-7721 cells[J]. ZHEN XU, MIN XIONG SHEN, DONG ZHU MA, LI YING WANG, XI LIANG ZHA Key Laboratory of Glycoconjugate Research, Ministry of Public Health, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China 2 Medical School, Huzhou Teacher’s College, Huzhou Zhejiang 313000, China.  Cell Research. 2003(05)
[7]Hepatic stem cells: existence and origin[J]. Ying Zhang Xue-Fan Bai Chang-Xing Huang The center of diagnosis and treatment for infectious diseases of PLA, Tang Du Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China.  World Journal of Gastroenterology. 2003(02)
[8]肝星狀細胞激活的細胞內(nèi)信號轉(zhuǎn)導[J]. 黃光存,張錦生.  世界華人消化雜志. 2001(09)
[9]細胞因子與肝再生[J]. 安永,別平.  世界華人消化雜志. 2001(05)
[10]Expression changes of activin A in the development of hepatic fibrosis[J]. Xin Huang1;Ding Guo Li1;Zhi Rong Wang1;Hong Shan Wei1;Ji Lin Cheng1;Yu Tao Zhan1;Xin Zhou1;Qin Fang Xu1;Xin Li2;Han Ming Lu1 1Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China2Ninth Hospital. Shanghai Second Medical University, Shanghai 200011. China.  World Journal of Gastroenterology. 2001(01)



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