第一部分構(gòu)建CDAA飲食誘導(dǎo)的小鼠NASH模型目的:構(gòu)建CDAA飲食誘導(dǎo)的NASH小鼠模型,為進(jìn)一步的深入研究做基礎(chǔ)。方法:選用6-8周齡雄性SPF級C57BL小鼠,經(jīng)適應(yīng)性喂養(yǎng)1周后分為正常普通飲食NF組、CDAA模型組、CSAA對照組直到第12周末結(jié)束。造模結(jié)束時,留取血清檢測生化指標(biāo):丙氨酸氨基轉(zhuǎn)化酶（ALT）、門冬氨酸氨基轉(zhuǎn)移酶（AST）、甘油三酯（TG）、尿素氮（BUN）、血糖（Blood Glucose）、總膽固醇（T-CHO）、總膽紅素（TBIL）的水平;肝組織經(jīng)石蠟包埋HE染色,鏡下觀察小鼠肝組織病理變化,天狼猩紅染色評價NASH模型纖維化程度。并應(yīng)用NAFLD活動度積分（NAFLD activity score,NAS）評分判斷NASH模型程度;ELISA方法檢測正常組、對照組、模型組外周血炎性因子PGE2、IL-6、INF-γ水平。結(jié)果:（1）經(jīng)純化CDAA模型飲食、CSAA對照飲食組喂養(yǎng)小鼠12周末其平均體重,肝指數(shù)與正常組無顯著差異（P>0.05）。模型組小鼠肝臟色澤變黃,邊緣較鈍,質(zhì)地較硬,彈性差。模型組外周血清ALT、AST、Glucose較正常組均明... 

【文章來源】：山西醫(yī)科大學(xué)山西省

【文章頁數(shù)】：150 頁

【學(xué)位級別】：博士

【部分圖文】：

實驗小鼠不同時間點體重的變化

圖 2-1 實驗小鼠不同時間點體重的變化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33

圖 2-1 實驗小鼠不同時間點體重的變化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33

【參考文獻(xiàn)】：
期刊論文
[1]Hepatic immune tolerance induced by hepatic stellate cells[J]. Ching-Chuan Hsieh,Chien-Hui Hung,Lina Lu,Shiguang Qian.  World Journal of Gastroenterology. 2015(42)
[2]Emerging roles of myeloid derived suppressor cells in hepatic inflammation and fibrosis[J]. Linda Hammerich,Frank Tacke.  World Journal of Gastrointestinal Pathophysiology. 2015(03)
[3]Prevalence of fatty liver disease and its risk factors in the population of South China[J]. Yong-Jian Zhou,Yu-Yuan Li,Min-Hu Chen,Pin-Jin Hu,Department of Gastroenterology,The First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province ,China Yong-Jian Zhou,Yu-Yuan Li,Yu-Qiang Nie,Sheng-Li Shi,Department of Gastroenterology and Hepatology,First Municipal People’s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,Guangdong Province,China Jin-Xiang Ma,Department of Prevention Medicine,Guangzhou Medical College,Guangzhou 510182,Guangdong Province,China Lun-Gen Lu,Department of Gastroenterology,Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai Jiaotong University School of Medicine,Shanghai 200001,China.  World Journal of Gastroenterology. 2007(47)



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