背景慢性乙型肝炎是我國(guó)常見的慢性傳染病之一,嚴(yán)重危害人民健康。慢性乙型肝炎（CHB）抗病毒治療的長(zhǎng)期目標(biāo)是達(dá)到病毒清除、延緩肝臟失代償、肝硬化和肝細(xì)胞癌的進(jìn)展及其并發(fā)癥的發(fā)生,進(jìn)而改善生活質(zhì)量和延長(zhǎng)存活時(shí)間。在所有的治療方案中,抗病毒治療是關(guān)鍵,同時(shí)隨著個(gè)體化抗病毒治療的推進(jìn),抗病毒治療療效中預(yù)測(cè)因素,特別是相關(guān)的宿主遺傳因素引起了人們的關(guān)注。本實(shí)驗(yàn)旨在探討雌激素受體α基因T29C多態(tài)性與慢性乙型肝炎患者干擾素-α抗病毒治療后發(fā)生早期應(yīng)答的相關(guān)性。方法對(duì)首次使用干擾素-α（IFN-α）抗病毒治療的100例慢性乙型肝炎患者的早期應(yīng)答情況進(jìn)行評(píng)估,并采用聚合酶鏈反應(yīng)-限制性片段長(zhǎng)度多態(tài)性法（PCR-RFLP）檢測(cè)雌激素受體α（ESR1）基因T29C多態(tài)性:T/T、T/C和C/C三個(gè)基因型,并按照此基因型分組分析了其與IFN-α早期應(yīng)答的相關(guān)性。結(jié)果（1）三個(gè)基因型組間的性別（χ2 = 1.148,?P = 0.563）、年齡（F = 1.153,? P=0.320）、治療前ALT水平（F = 0.862,?P = 0.441）、治療前HBV病毒載量? （Z = 1.961,? P =0.1... 

【文章來源】：安徽醫(yī)科大學(xué)安徽省

【文章頁數(shù)】：61 頁

【學(xué)位級(jí)別】：碩士

【部分圖文】：

PCR-RFLP檢測(cè)ESR1基因T29C多態(tài)性電泳結(jié)果

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